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Thursday, January 19, 2012
SUPERFICIAL MYCOSES




Thursday, January 19, 2012
WHAT ARE SUPERFICIAL
        MYCOSES?
              Superficial mycoses are cosmetic fungal infections of the skin
              or shaft

                    No living tissue is invaded

                    No cellular response from the host

                    No pathological changes elicited

              These infections are often so innocuous that patients are often
              unaware of their condition.


Thursday, January 19, 2012
TINEA VERSICOLOR
        (Pityriasis versicolor)
          Malassezia furfur: commonly known
          as “an-an”

          Direct microscopic examination of
          alkali stain (KOH or PAS) treated
          with skin scrapings: “spaghetti and
          meatballs”

          CONFIRMATION: cultures are not
          routinely done

          ORGANISMS: lipophilic =
          requires fatty acid-supplemented      http://missinglink.ucsf.edu

Thursday, January 19, 2012
TINEA VERSICOLOR
        (Pityriasis versicolor)
          Lesions occur most frequently on
          the upper torso, arms, and
          abdomen as discrete hyper – or
          hypopigmented macular lesions.

          They scale very easily, giving the
          affected area a dry, or chalky
          appearance.

            Sometimes, lesions appeared to be
          elevated and folliculitis may occur           http://
          if hair follicles were involved.      dermimages.med.jhmi.edu

Thursday, January 19, 2012
TREATMENT


              1% selenium sulfide
              applied every other
              day for 15 minutes
              and then washed off




Thursday, January 19, 2012
TINEA NIGRA
        (Exophiala werneckii)
          usually asymptomatic and consist
          of well demarcated macular
          lesions (discolored spots on the
          skin that are not raised above the
          surface) that enlarge by peripheral
          extension

          brown to black lesions = often
          seen at the sole of the foot or at the
          palmar surface of the hand (other
          body areas may also be affected)


Thursday, January 19, 2012
TINEA NIGRA
        (Exophiala werneckii)
          Diagnosis: darkly pigmented
          yeast like cells and hyphal
          fragments in microscopic
          examination of KOH tested
          scrapings taken from affected
          area

                confirmatory test is made
                by culture


Thursday, January 19, 2012
TREATMENT
              undecyclenic acid

              keratolytic agent

              Whitfield’s ointment
              (daily)

              Tincture of iodine, 2%
              salicylic acid or 3%
              sulfur
Thursday, January 19, 2012
WHITE PIEDRA
        (Trichosporon beigelii)

                soft, white to light
                brown nodules

                form on axillary,
                pubic, beard and
                scalp hair

                relapse common

Thursday, January 19, 2012
WHITE PIEDRA
        (Trichosporon beigelii)

                hyaline septate hyphae that
                fragment into oval or
                rectangular arthroconida

                blastospores can also occur
                at one or more points on the
                arthrospores


Thursday, January 19, 2012
TREATMENT & DIAGNOSIS
           DIAGNOSIS

                 direct microscopic exam of hairs

                 culture: asexual phase of the fungus

           TREATMENT

                 shaving or cutting the infected hair

                 Topical fungicides: bichloride of mercury
                 (1:200)

                 Benzoic and salicylic acid combinations

                 3% sulfur ointments


Thursday, January 19, 2012
BLACK PIEDRA
        (Piedraia hortae)
                hard black nodules formed
                around the scalp hair
                (carbonaceous; can house an
                asci)

                thick-walled closely septate
                hyphe, with chlamydoconidia

                growth is dimorphic – hyphae
                arthroconidia, and
                blastoconidia.

Thursday, January 19, 2012
DIAGNOSIS & TREATMENT

                 DIAGNOSIS:

                       direct microscopic examination of
                       affected hair

                       culture: Saborauds dextrose agar

                 TREATMENT

                       therapy: shaving and cutting the infected
                       hair

                       topical fungicides, bichloride of mercury

                       Benzoic acid/salicylic acid combinations


Thursday, January 19, 2012
BLACK VERSUS WHITE

         CHARACTERISTIC          BLACK PIEDRA              WHITE PIEDRA

         Etiology              Piedraia hortae           Trichosporon beigelii

                               Over and around the
                                                         On the surface of the
                               hair shaft , hard,
         Nodules                                         hair shaft, softer, easy to
                               difficult to detach from
                                                         separate from hair
                               the hair

                               Tightly packed
                                                         Arthrospores and
         Direct Examinations   dichotomous branched
                                                         blastopores can be seen
                               hyphae
         Presence of asci      Positive                  Negative

Thursday, January 19, 2012
CUTANEOUS MYCOSES




Thursday, January 19, 2012
CUTANEOUS MYCOSES :
        DERMATOPHYTES
           superficial fungal infections of the skin, hair and nails

           may stimulate immune response

           no living tissues are involved = generally restricted to
           the keratinized layers of the integument and its
           appendages

           NOTE: a variety of pathological changes may occur in
           the host because of the presence of infectious agent and
           its metabolic product

Thursday, January 19, 2012
CLASSIFICATION OF
        DERMATOPHYTES




Thursday, January 19, 2012
REPRESENTATIVE
        DERMATOPHYTES




Thursday, January 19, 2012
CLINICAL FEATURES OF
        DERMATOPHYTE INFECTIONS
                                                                                         FUNGI MOST
                                                                 CLINICAL
                SKIN DISEASE      LOCATION OF LESIONS                                    FREQUENTLY
                                                                APPEARANCE
                                                                                         RESPONSIBLE

                                                         clinical patches with
                                                             advancing red,            Microsporum canis,
           TINEA CORPORIS
                                  Non-hairy smooth skin vesiculated border and           Trichophyton
              (ringworm)
                                                            central scaling =           mentagrophytes
                                                                pruritic
                                                              acute: itching, red
                                                                                           T. rubrum
                                   interdigital spaces on         vesicular
               TINEA PEDIS                                                             T. mentagrophytes
                                  feet of persons wearing
               (athlete’s foot)                                                         Epidermophyton
                                            shoes              chronic: itching,
                                                                                           floccosum
                                                               scaling, fissures

                                                             erythematousnscaling          T. rubrum
         TINEA CRURIS (jock
                                          groin             lesion in intertriginous   T. mentagrophytes
                itch)
                                                                areas = pruritic          E. floccosum



Thursday, January 19, 2012
CLINICAL FEATURES OF
        DERMATOPHYTE INFECTIONS
                                                                               FUNGI MOST FREQUENTLY
                 SKIN DISEASE    LOCATION OF LESIONS   CLINICAL APPEARANCE
                                                                                    RESPONSIBLE


                                      scalp hair
                                                     circular bald patches
                                ENDOTHRIX: fungus with short hair stubs or
                                                                                      M. canis
             TINEA CAPITIS        inside hair shaft broken hair within hair
                                                                                    T. tonsurans
                                                    follicles = Mircosporum-
                                EXOTHRIX: fungus on infected hair fluoresce
                                   surface of hair

                                                           edematous,                T. rubrum
             TINEA BARBAE            beard hair
                                                       erythematous lesion       T. mentagrophytes

                                                         nails thickened or
                                                        crumbling distally,          T. rubrum
           TINEA UNGUIUM
                                         nail           discolored = usally      T. mentagrophytes
            (onychomycosis)
                                                       associated with Tinea        E. floccosum
                                                                pedis

Thursday, January 19, 2012
TINEA CAPITIS
        (ringworm of the scalp)




Thursday, January 19, 2012
TINEA BARBAE




Thursday, January 19, 2012
TINEA CORPORIS
        (ringworm)




Thursday, January 19, 2012
TINEA CRURIS
        (jock itch)




Thursday, January 19, 2012
TINEA PEDIS
        (athlete’s foot))




Thursday, January 19, 2012
TINEA MANUUM




Thursday, January 19, 2012
TINEA UNGUIUM
        (onychomycosis)




Thursday, January 19, 2012
Microsporum audoinii




                             Tinea capitis = intercalary
                                chlamydoconidium
Thursday, January 19, 2012
Microsporum canis




                             Tinea capitis, corporis and barbae =
                                  pyriform shaped conidia
Thursday, January 19, 2012
Microsporum cookei




                             saprophytic = may be found in animal hairs
                                         (dogs and rodents)
Thursday, January 19, 2012
Microsporum ferrugineum




                             Tinea capitis, corporis = bamboo-shaped
                                               hyphae
Thursday, January 19, 2012
Microsporum gypseum




                             Tinea capitis, corporis, favosa = ellipsoidal;
                                      4-6 cells in macroconidia
Thursday, January 19, 2012
Microsporum gallinae




                             white comb lesion in chicken = blunt tip;
                                     cylindrofusiform-shaped
Thursday, January 19, 2012
Microsporum nanum




                             Tinea capitis, corporis = ringworm of pigs

Thursday, January 19, 2012
Epidermophyton floccosum




                             Tinea cruris, pedis, unguium = rarely
                                          invades hair
Thursday, January 19, 2012
Microsporum equinum




                             Tinea in horses = large chain of spores

Thursday, January 19, 2012
Microsporum fulvum




                             Tinea capitis = bullet-shaped septa

Thursday, January 19, 2012
Trichophyton mentagrophytes




                             all types of tinea = spiral hyphae “en
                                             grappe”
Thursday, January 19, 2012
Trichophyton rubrum




                             all types of tinea = “en thyrse”

Thursday, January 19, 2012
Trichophyton veruccosum




                             Tinea corporis, barbae = ringworm in cattle
                                      (culture requires inositol)
Thursday, January 19, 2012
Trichophyton tonsurans




                             Tinea capitis, corporis - originates from
                                             aborigines
Thursday, January 19, 2012
Trichophyton schoenleinii




                             Tinea capitis, favosa, corporisfavus = scutula
                                         (permanent hair loss)
Thursday, January 19, 2012
Trichophyton violaceum




                             Tinea capitis, favosa, corporis = black dot
                                      (deep violet in culture)
Thursday, January 19, 2012
Trichophyton concentricum




                             Tinea imbricata = resembles antler tip

Thursday, January 19, 2012
ECOLOGICAL NICHE OF
        CUTANEOUS MYCOSES




Thursday, January 19, 2012
COMPARING HAIR
        INFECTIONS




Thursday, January 19, 2012

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Superficial:cutaneous mycoses

  • 3. WHAT ARE SUPERFICIAL MYCOSES? Superficial mycoses are cosmetic fungal infections of the skin or shaft No living tissue is invaded No cellular response from the host No pathological changes elicited These infections are often so innocuous that patients are often unaware of their condition. Thursday, January 19, 2012
  • 4. TINEA VERSICOLOR (Pityriasis versicolor) Malassezia furfur: commonly known as “an-an” Direct microscopic examination of alkali stain (KOH or PAS) treated with skin scrapings: “spaghetti and meatballs” CONFIRMATION: cultures are not routinely done ORGANISMS: lipophilic = requires fatty acid-supplemented http://missinglink.ucsf.edu Thursday, January 19, 2012
  • 5. TINEA VERSICOLOR (Pityriasis versicolor) Lesions occur most frequently on the upper torso, arms, and abdomen as discrete hyper – or hypopigmented macular lesions. They scale very easily, giving the affected area a dry, or chalky appearance. Sometimes, lesions appeared to be elevated and folliculitis may occur http:// if hair follicles were involved. dermimages.med.jhmi.edu Thursday, January 19, 2012
  • 6. TREATMENT 1% selenium sulfide applied every other day for 15 minutes and then washed off Thursday, January 19, 2012
  • 7. TINEA NIGRA (Exophiala werneckii) usually asymptomatic and consist of well demarcated macular lesions (discolored spots on the skin that are not raised above the surface) that enlarge by peripheral extension brown to black lesions = often seen at the sole of the foot or at the palmar surface of the hand (other body areas may also be affected) Thursday, January 19, 2012
  • 8. TINEA NIGRA (Exophiala werneckii) Diagnosis: darkly pigmented yeast like cells and hyphal fragments in microscopic examination of KOH tested scrapings taken from affected area confirmatory test is made by culture Thursday, January 19, 2012
  • 9. TREATMENT undecyclenic acid keratolytic agent Whitfield’s ointment (daily) Tincture of iodine, 2% salicylic acid or 3% sulfur Thursday, January 19, 2012
  • 10. WHITE PIEDRA (Trichosporon beigelii) soft, white to light brown nodules form on axillary, pubic, beard and scalp hair relapse common Thursday, January 19, 2012
  • 11. WHITE PIEDRA (Trichosporon beigelii) hyaline septate hyphae that fragment into oval or rectangular arthroconida blastospores can also occur at one or more points on the arthrospores Thursday, January 19, 2012
  • 12. TREATMENT & DIAGNOSIS DIAGNOSIS direct microscopic exam of hairs culture: asexual phase of the fungus TREATMENT shaving or cutting the infected hair Topical fungicides: bichloride of mercury (1:200) Benzoic and salicylic acid combinations 3% sulfur ointments Thursday, January 19, 2012
  • 13. BLACK PIEDRA (Piedraia hortae) hard black nodules formed around the scalp hair (carbonaceous; can house an asci) thick-walled closely septate hyphe, with chlamydoconidia growth is dimorphic – hyphae arthroconidia, and blastoconidia. Thursday, January 19, 2012
  • 14. DIAGNOSIS & TREATMENT DIAGNOSIS: direct microscopic examination of affected hair culture: Saborauds dextrose agar TREATMENT therapy: shaving and cutting the infected hair topical fungicides, bichloride of mercury Benzoic acid/salicylic acid combinations Thursday, January 19, 2012
  • 15. BLACK VERSUS WHITE CHARACTERISTIC BLACK PIEDRA WHITE PIEDRA Etiology Piedraia hortae Trichosporon beigelii Over and around the On the surface of the hair shaft , hard, Nodules hair shaft, softer, easy to difficult to detach from separate from hair the hair Tightly packed Arthrospores and Direct Examinations dichotomous branched blastopores can be seen hyphae Presence of asci Positive Negative Thursday, January 19, 2012
  • 17. CUTANEOUS MYCOSES : DERMATOPHYTES superficial fungal infections of the skin, hair and nails may stimulate immune response no living tissues are involved = generally restricted to the keratinized layers of the integument and its appendages NOTE: a variety of pathological changes may occur in the host because of the presence of infectious agent and its metabolic product Thursday, January 19, 2012
  • 18. CLASSIFICATION OF DERMATOPHYTES Thursday, January 19, 2012
  • 19. REPRESENTATIVE DERMATOPHYTES Thursday, January 19, 2012
  • 20. CLINICAL FEATURES OF DERMATOPHYTE INFECTIONS FUNGI MOST CLINICAL SKIN DISEASE LOCATION OF LESIONS FREQUENTLY APPEARANCE RESPONSIBLE clinical patches with advancing red, Microsporum canis, TINEA CORPORIS Non-hairy smooth skin vesiculated border and Trichophyton (ringworm) central scaling = mentagrophytes pruritic acute: itching, red T. rubrum interdigital spaces on vesicular TINEA PEDIS T. mentagrophytes feet of persons wearing (athlete’s foot) Epidermophyton shoes chronic: itching, floccosum scaling, fissures erythematousnscaling T. rubrum TINEA CRURIS (jock groin lesion in intertriginous T. mentagrophytes itch) areas = pruritic E. floccosum Thursday, January 19, 2012
  • 21. CLINICAL FEATURES OF DERMATOPHYTE INFECTIONS FUNGI MOST FREQUENTLY SKIN DISEASE LOCATION OF LESIONS CLINICAL APPEARANCE RESPONSIBLE scalp hair circular bald patches ENDOTHRIX: fungus with short hair stubs or M. canis TINEA CAPITIS inside hair shaft broken hair within hair T. tonsurans follicles = Mircosporum- EXOTHRIX: fungus on infected hair fluoresce surface of hair edematous, T. rubrum TINEA BARBAE beard hair erythematous lesion T. mentagrophytes nails thickened or crumbling distally, T. rubrum TINEA UNGUIUM nail discolored = usally T. mentagrophytes (onychomycosis) associated with Tinea E. floccosum pedis Thursday, January 19, 2012
  • 22. TINEA CAPITIS (ringworm of the scalp) Thursday, January 19, 2012
  • 24. TINEA CORPORIS (ringworm) Thursday, January 19, 2012
  • 25. TINEA CRURIS (jock itch) Thursday, January 19, 2012
  • 26. TINEA PEDIS (athlete’s foot)) Thursday, January 19, 2012
  • 28. TINEA UNGUIUM (onychomycosis) Thursday, January 19, 2012
  • 29. Microsporum audoinii Tinea capitis = intercalary chlamydoconidium Thursday, January 19, 2012
  • 30. Microsporum canis Tinea capitis, corporis and barbae = pyriform shaped conidia Thursday, January 19, 2012
  • 31. Microsporum cookei saprophytic = may be found in animal hairs (dogs and rodents) Thursday, January 19, 2012
  • 32. Microsporum ferrugineum Tinea capitis, corporis = bamboo-shaped hyphae Thursday, January 19, 2012
  • 33. Microsporum gypseum Tinea capitis, corporis, favosa = ellipsoidal; 4-6 cells in macroconidia Thursday, January 19, 2012
  • 34. Microsporum gallinae white comb lesion in chicken = blunt tip; cylindrofusiform-shaped Thursday, January 19, 2012
  • 35. Microsporum nanum Tinea capitis, corporis = ringworm of pigs Thursday, January 19, 2012
  • 36. Epidermophyton floccosum Tinea cruris, pedis, unguium = rarely invades hair Thursday, January 19, 2012
  • 37. Microsporum equinum Tinea in horses = large chain of spores Thursday, January 19, 2012
  • 38. Microsporum fulvum Tinea capitis = bullet-shaped septa Thursday, January 19, 2012
  • 39. Trichophyton mentagrophytes all types of tinea = spiral hyphae “en grappe” Thursday, January 19, 2012
  • 40. Trichophyton rubrum all types of tinea = “en thyrse” Thursday, January 19, 2012
  • 41. Trichophyton veruccosum Tinea corporis, barbae = ringworm in cattle (culture requires inositol) Thursday, January 19, 2012
  • 42. Trichophyton tonsurans Tinea capitis, corporis - originates from aborigines Thursday, January 19, 2012
  • 43. Trichophyton schoenleinii Tinea capitis, favosa, corporisfavus = scutula (permanent hair loss) Thursday, January 19, 2012
  • 44. Trichophyton violaceum Tinea capitis, favosa, corporis = black dot (deep violet in culture) Thursday, January 19, 2012
  • 45. Trichophyton concentricum Tinea imbricata = resembles antler tip Thursday, January 19, 2012
  • 46. ECOLOGICAL NICHE OF CUTANEOUS MYCOSES Thursday, January 19, 2012
  • 47. COMPARING HAIR INFECTIONS Thursday, January 19, 2012