histo lap

1. BENGHAZI UNIVERSITY
FACULTY OF MEDICINE
DEPARTMENT OF HISTOLOGY
FIRST YEAR MEDICAL PRELAB PART III
Prepared by:
Dr. Hala El.Alam.
Dr. Manal Eljerby.
Dr. Lobna Alferjani.
1

2. Prelab 9
Nervous tissue I

3. Slides of lab 9 ..
1. Multipolar nerve cells in spinal cord. X40
2. Pyramidal nerve cells in cerebral cortex. X40
3. Pyriform nerve cells in cerebellum . X40
4. Astrocyte. X40

4. 1.Multipolar nerve cells in spinal cord
1. Cell body (perikarion) : Pyramidal in shape .
2. nucleus : Prominent nucleolus .
3. Single axon .
4. Multiple dendrites .

5. 1.Multipolar nerve cells in spinal cord

6. Multipolar nerve cells in spinal cord x40

7. 2.Pyriform nerve cells in cerebellum.
1. Called Purkinji cells.
2. Multipolar cell.
3. Arranged in raw between granular & molecular
layer .
4. Flask shaped cell bodies .
5. One axon &many branched dendrites

8. 2.Pyriform nerve cells in cerebellum.

9. Pyriform nerve cells in cerebellum X40
Molecular layer
Granular
layer

10. 3.Pyramidal nerve cells in cerebral cortex X40
1. Large pyramidal cell body.
2. Large vesicular nucleus.
3. Multiple dendrites .

11. 3.Pyramidal nerve cells in cerebral cortex
X40

12. 4.Astrocyte
1. Star shaped cell body.
2. Large nucleus.
3. Stained black with silver.
4. Numerous long branched processes end with feet
like expansions on blood vessels.

13. 4.Astrocyte
Astrocyte

14. Astrocyte × 40.

15. Prelab 10
Nervous tissue II

16. Slides of prelab 10 ..
1. Oligodendrocyte . X40
2. Spinal ganglion . X40
3. Sympathetic ganglion . X40
4. Ependyma . X40
5. Nerve trunk . X40

17. 1. Oligodendrocyte ..
1.small cell body .
2. few processes .
3. large dark nucleus .
4. form myelin sheaths in
CNS .

18. Oligodendrocyte .. X40
Cell
body
Dendrites

19. 2&3 Spinal & sympathetic ganglion
Spinal ganglion Sympathetic ganglion
1. Contain Pseudounipolar neurons. 1. Multipolar neurons.
2. Large with central nucleus. 2. Small numerous with eccentric
3. In groups. nucleus.
4. Completely surrounded by satellite 3. Scattered.
cells.
4. Have few less prominent
5. Separated by thick myelinated satellite cells.
fibers.
5. Separated by Unmyelinated
6. Oval shape ,Thick capsule.
nerve cells.
7. They are present beside spinal cord
in both sides . 6. Round shape ,Thin capsule.
7. Related to autonomic nervous

20. Spinal & sympathetic ganglion
Sympathetic ganglion
Spinal ganglion

21. 2.Spinal ganglion … X40
C.T capsule
Pseudounipolar N.
Stellate cells
Myelinated n. fibers

22. Spinal ganglion X100
Pseudounipolar neuron
Central nucleus
&prominent nucleolus .
Stellate cells .

23. Spinal ganglion X40

24. 3.Sympathetic ganglion… X40
Multipolar N .
Unmyelinated N.
fibers

25. 4.Ependyma ..
1.single layer of simple
cuboidal epithelium .
2. lines the ventricles of brain
& central canal of spinal cord
.
3.Ciliated and non ciliated .

26. 4.Ependyma ..

27. Ependyma x40
Cilia

28. 5.Nerve trunk ..
1.. Nerves are surrounded by
epineurium .
2. Each bundle (or fascicle) of nerve fibers is surrounded by
perineurium .
3. Each nerve fiber within each bundle is individually
surrounded by endoneurium

29. 5.Nerve trunk ..

30. Nerve trunk .. X40
Perineurium
Endoneurium
Nerve axon

31. Prelab 12
Lymphatic tissue

32. Slides of prelab
1. Thymus gland . X40.
2. Lymph node . X40.
3. Spleen . X40.
4. Palatine tonsils . X40.
5. Payers patches . X40.

33. Thymus
Hassall corpuscle

34. Thymus
a- Capsule and septa of C.T tissue
b- Cortex and medulla
c- Medulla contains Hassall's corpuscles

36. Lymph node
• C.T capsule and
Trabeculae
• b- Cortex : lymphatic
nodules with germinal
center
• c- Medulla : Medullary
cords and sinuses.

37. Lymph node

39. cortex
Medulla

40. Spleen
• a - C.T capsule and Trabeculae
• b- White pulp ( lymphatic nodules & central artery)
• c-Red pulp (splenic cord and splenic venous sinuses)

42. Spleen

43. Spleen

44. Spleen

45. White pulp
1. Lymphatic
nodule with
germinal center.
2. Central artery .

46. Palatine Tonsils
• Tonsillar crypts.
• Lymphatic nodules
• Stratified nonkeratinized epithelial covering.

47. Palatine Tonsils
• Tonsillar crypts.
• Lymphatic nodules
• Stratified nonkeratinized epithelial covering.

48. Palatine Tonsils

49. Palatine Tonsil
Lymphatic nodules
Mucus glands
Crypt
Epithelium

50. Peyer`s Patches
• Are aggregations of
lymphatic nodules.
• Located in the lamina
propria of the wall of
the ileum opposite to
the mesentery.

51. Payer’s Patch

52. Payer’s Patch

53. Prelab 13
Skin

54. Slides for the prelab
• 1. thick skin x10
• 2. thin skin x10
• 3. hair follicle ( cross section) x40
• 4.negroid skin x40.

55. Thick skin
1.In palms & soles.
2 Keratinized stratified squamous epithelium.
3.Thick epidermis.
4.Many sweat gland.
5.No hair or sebaceous gland.

57. Thick Skin

58. Thin skin
1- Epidermis : thin.
2- Dermis :thick
3- Stratified squamous Keratinized epithelium
4- Hair follicles
5- Sebaceous and sweat glands

59. Thin skin

60. epithelium
Thin skin
Errector Pillae muscle
Sebaceous gland
Hair follicle
Sweat gland

61. Thin skin x10

62. Thin skin x10

63. Hair follicle (cross section)
1.Cortex
2.medulla
3. inner & outer root sheet.
4.connective tissue sheet.

64. Hair follicle (cross section) x40

65. Negroid skin
1. Stratified squamous Keratinized
epithelium.
2. Melanin pigment

66. Negroid skin X40

67. Negroid skin X40

68. Thick Skin Thin Skin
Site Palms & soles Whole body except palms and soles
Epidermis Thicker Thinner
Hair follicles & Absent Present
sebaceous glands
papillary ridges Characteristic Not characteristic.
(finger prints)

69. Prelab 14
Vascular system

70. Slides of this lab
• 1. aorta (as an example of
large artery).
• 2.inferior vena cava(as an
example of large vein)
• 3.medium sized artery& vein.

71. Aorta
1- Tunica intima: endothelium and subedothelium
connective tissue.
2.internal elastic lamina.
3- Tunica media: thick, mainly of elastic fiber with
smooth muscle cells.
4- Tunica adventitia: Thin and is composed of
loose fibroelastic connective tissue, contain vasa
vasorum.

72. Aorta

73. aorta

74. Veins (Inferior vena cava)
1.Tunica intima: Thin, endothelium and
subedothelium.
2.Tunica media: Thin and contain few
smooth muscle fiber.
3.Tunic adventitia: Thickest of the three
tunicas, contains coarse collagen fibers,
elastic fibers and longitudinal bundles of
smooth muscle.
4. no internal or external elastic lamina

76. Inferior vena cava

77. Inferior vena cava

78. Medium sized artery Medium sized vein
wall Thick with narrow Thin with wide
lumen lumen.
Tunica intima thick folded with thin
internal elastic
lamina
Tunica media thick, formed of Thin, thin and
circularly arranged formed of smooth
smooth muscles muscle cells
separated by
Collagenous fibers,
and few elastic
connective tissue
Tunica adventitia thin thick
Internal elastic Well developed absent
lamina
External elastic May be present absent
lamina

79. Medium sized artery and vein