4. TRANSCRIPTION RNA PROCESSING DNA RNA transcript 3 5 RNA polymerase Poly-A Poly-A RNA transcript (pre-mRNA) Intron Exon NUCLEUS Aminoacyl-tRNA synthetase AMINO ACID ACTIVATION Amino acid tRNA CYTOPLASM Poly-A Growing polypeptide 3 Activated amino acid mRNA TRANSLATION Cap Ribosomal subunits Cap 5 E P A A Anticodon Ribosome Codon E The central dogma DNA RNA PROTEIN
7. (a) Tobacco plant expressing a firefly gene (b) Pig expressing a jellyfish gene
8. Kary Mullis – Genius or eccentric? ‘ Science grows like a weed every year’ ‘ Each of us have things and thoughts and descriptions of an amazing universe in our possession that kings in the 17th Century would have gone to war to possess’ ‘ Science consistently produces a new crop of miraculous truths and dazzling devices every year’ ‘ I have had an encounter with an extraterrestrial in the form of a fluorescent raccoon’ ??????? You can read his Nobel lecture here: http://nobelprize.org/nobel_prizes/chemistry/laureates/1993/mullis-lecture.html
9. PCR – in the dark ages 8 BORING hours per PCR! 95º C 5 min 35 times 55º C 3 min 72º C 5 min
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13. The temperature profile of a PCR cycle is controlled by the thermal cycler program which results in a near exponential increase in PCR product accumulation for about the first 30 cycles. The 3 Os!!!!!
