1. “ FAST, LOW-COST DEVICE
USES THE CLOUD TO SPEED
UP DIAGNOSTIC TESTING
FOR HIV AND MORE” AND
“NOVEL TEST STREAMLINES
TESTING FOR HUNTINGTON
DISEASE”
MANUELA JIMÉNEZ
OBANDO
MEDICINE STUDENT UPB 2013

4. There are many
techniques that are used
in molecular biology, for
example, PCR
(polymerase chain
reaction) or ELISA.
PCR pretends to amplify
DNA o RNA, using
enzymes in vitro, and is
useful in the detection of
genetical disease like
Huntington.

5. ELISA is a low-cost immunoassay
technique that finding antibodies
or antigens, is really useful in the
detection of different diseases.

6. “FAST LOW-COST DEVICE
USES THE CLOUD TO SPPED
UP DIAGNOSTIC TESTING
FOR HIV AND MORE”
Clinical Chemistry, January
18,2013

7. “FAST LOW-COST DEVICE USES THE CLOUD TO
SPPED UP DIAGNOSTIC TESTING FOR HIV AND
MORE”
Samuel K. Sia developed
an advanced technique that
he proposed to provide a It consists of a handheld
mobile device that only by
disgnostic service with a
picking a finger and getting a
lab-quality, to people in sample, can give the results
remote areas of the world , in just 15 minutes (faster
who can´t go to a hospital than ELISA).
facility.
This test allows a
real-time
synchronization of
diagnostic results
with patient records
in the cloud.

8. “FAST LOW-COST DEVICE USES THE CLOUD TO
SPPED UP DIAGNOSTIC TESTING FOR HIV AND
MORE”
The possibility to give
quick results allows
real-time data upload,
resulting in easier
epidemiological
analysis; this is one of
the contributions to the
medical world: the
prevention of some
diseases (HIV for
example).

9. “FAST LOW-COST DEVICE USES THE CLOUD TO
SPPED UP DIAGNOSTIC TESTING FOR HIV AND
MORE”
Sia has now other bigger
objetives with his “lab on a “To implement an
chip”: antenatal care
panel for
diagnosing HIV and
sexually transmitted
diseases for
pregnant women in
Rwanda”

10. “FAST LOW-COST DEVICE USES THE CLOUD TO
SPPED UP DIAGNOSTIC TESTING FOR HIV AND
MORE”
I think what Sia is doing is a great
contribution to the development of
medical technologies, because it brings
the possibility of getting a diagnosis to
OBSERVATION:
OBSERVATION: people who can´t go to a health facility or
who live in undeveloped countries. On
the other side, this technology facilitates
the information to epidemiological
studies.

11. “NOVEL TEST
STREAMLINES TESTING
FOR HUNTINGTON
DISEASE”
The Journal of Molecular
Diagnostics, Volume 15,
Issue 2 (March 2013)
published by Elsevier.

12. “NOVEL TEST STREAMLINES TESTING
FOR HUNTINGTON DISEASE”
This disease is a neurodegenerative
disorder (oringinated by the
HUNTINGTON Huntingtina, a protein encoded by
repeted sequences of “CAG”)
DISEASE
This protein accumulation affects
specially the brain and the expression of
symptoms is directly related with the
number of repetitions.
Symptoms are often related with problems in
mood or cognition, diminished muscle
coordination, with consequences which are
evident on walking, speaking and other daily
life activities. This people also has changes in
personality and thinking skills.
Neurons with Nuclear inclusions

13. “NOVEL TEST STREAMLINES TESTING
FOR HUNTINGTON DISEASE”
A new test :: TP PCR
A new test TP PCR
Generates
accurate results by The study used 246 samples that were
counting the previously analyzed with other methods
repetitions of the (with possitive and negative results for
sequence and HD), and the samples results coincided.
doesn´t need
additional tests

14. “NOVEL TEST STREAMLINES TESTING
FOR HUNTINGTON DISEASE”
The TP PCR based its
Even 101 repetitions could
analysis in the fact than 39
be accurately sized with TP
or more repetitions of “CAG”
PCR.
determinates the risk of
Also samples that were
developing HD.
really difficult to analyze
with other methods, and
were considered a
challenging, had easy and
Another advantage of TP PCR accurate results with this
is the ability to identify the new test.
homozygous normal samples
and avoid further tests.
However, in children further
tests are recomended.

15. “NOVEL TEST STREAMLINES TESTING
FOR HUNTINGTON DISEASE”
I think that using TP PCR it is
possible to reduce the number of
OBSERVATION: false negative, that would be really
useful for people who has HD,
because it is a grave disease that
affect in a big way the life of
patients who suffer from it; they
can develop diminished muscle
coordination that is evident on
walking and speaking during the
daily life. This people also have
changes in personality and
thinking skills.

16. MEDICAL UTILITY
The easier, and more
effective diagnosis of a
lot of diseases like
Huntington.

17. MEDICAL UTILITY
Reduce the number of false
negatives in some diseases.

18. MEDICAL UTILITY
…And gives a faster way to
prevent and start
a treatment in positive
cases.

19. MEDICAL UTILITY
Gives the possibility of a
diagnosis and control tests to
places in the world without access
to specialized laboratories. And
do it with a low-cost and fast way.

20. BIBLIOGRAFÍA
 Martínez,Lina; Vargas,Natalia; Toro,Eduardo;
Pamplona,Ana; Quevedo,Esteban.“Biología molecular”.Ed7.
2012. Pp.121-127
 Mohamed Jama, Alison Millson, Christine E. Miller, Elaine
Lyon. Triplet Repeat Primed PCR Simplifies Testing for
Huntington Disease. The Journal of Molecular Diagnostics,
2013; DOI: 10.1016/j.jmoldx.2012.09.005
 C. D. Chin, Y. K. Cheung, T. Laksanasopin, M. M. Modena,
S. Y. Chin, A. A. Sridhara, D. Steinmiller, V. Linder, J.
Mushingantahe, G. Umviligihozo, W. El-Sadr, S. K.
Sia. Mobile Device for Disease Diagnosis and Data
Tracking in Resource-Limited Settings. Clinical
Chemistry, 2013; DOI: 10.1373/clinchem.2012.199596