Dna Extraction Principles

DNA Extraction Principles How do you get DNA out of a whole organism?

How do you get DNA out of a strawberry? Plant Cell Wall: cellulose and protein matrix Cell membrane: lipids and protein Plant Cell Cytoplasm: proteins etc Nucleus: DNA & protein

What does the detergent and NaCl do to the cell parts? Mashing the berry disrupts the cells and membranes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

What does detergent and NaCl do? ,[object Object],[object Object],How do we separate the clumps of cells from the small molecules?

Filtering -Clumps of cellular debris get stuck in the filter. -Small molecules flow thru. How do we get the DNA to separate from the pink liquid?

Precipitate the DNA with cold alcohol ,[object Object],[object Object],[object Object]

Summary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

DNA Properties ,[object Object],[object Object],[object Object],[object Object],[object Object]

Dna Extraction Principles

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