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Chapter
   3
Cell Structure and
     Function
Section 3-1

The History of the Cell Theory and
          Microscopes
Historical View of the Cell Theory
• As science improves, so do improvements
  in scientific instruments, and improved
  scientific instruments lead to new
  discoveries.
1590 – Zacharias Janssen
• Built first simple microscope (one
  set of lenses)
1670’s Anton Van Leeuwenhoek
• Made improvements
• 270x magnification
• Saw bacteria, protozoa,
  sperm cells, red blood
  cells and yeast cells
1665 – Robert Hooke
• Produced a compound microscope
• Saw hollow boxes and named them
  “cells”
1831 – Robert Brown
• Saw central structure in plant cells,
  called this structure a nucleus
1838 – Matthias Schleiden
• Concluded that all plants are
  made of cells
1838 – Theodor Schwann
• Concluded that all animals are
  made of cells
1839 – Johannes Purkinje
• Stated that “the cell is the unit of
  function of life”
1858 – Rudolf Virchow
• Concluded that “ cells come only from
  previously existing cells”
The Cell Theory
1. All living things are composed of
   cells
2. Cells are the basic units of
   structure and function
3. New cells are produced from
   existing cells
Compound Microscope
• Contains a
  combination of two
  lenses
Eyepiece or ocular lens
• Lens closest to the
  eye
Objective Lens
• Lens closest to the
  specimen or object
How to figure out magnification
• Eyepiece   X   objective   =   Magnification
•   10x      X
•   10x      X
•   10x      X
Nosepiece
• Objectives fastened
  to this revolving
  structure
Stage
• Where you place
  your slide
Stage clips
• Hold slide in place
Body Tube
• Light travels through
  here
Light Source
• Provides light
Diaphragm
• Regulates the light
  reaching the
  objective lens
Base
• Supports the
  microscope
Arm
• Holds the body
  tube, acts as a
  handle
Coarse focusing knob
• Used to focus under
  low power only
Fine focusing knob
• Used to focus under
  high power only
A microscope reverses and inverts the
    image of an object seen under it
Before
viewing
under the
microscope        e

While
viewing           e
under the
microscope
Things moving under a microscope are
actually moving in the opposite direction
Things moving under a microscope are
actually moving in the opposite direction
Stereomicroscope
• Binocular
  microscope
• Object is seen in
  3-D
• Only used to see
  large objects
• Does not reverse or
  invert images
Transmission Electron Microscope
• Uses a beam of
  electrons instead of light
  rays
• 200,000x magnification
• tissues have to be sliced
  really thin, dry and in a
  vacuum chamber
• can’t be used with living
  material
Scanning Electron Microscope
• provides images with 3-D quality
• can’t be used with living material
Micromanipulator
• Used to dissect
  cells, can remove
  nuclei
• Knowledge of cells
  has been increased
  by manipulation and
  dissection of cells
High – Speed Centrifuge
• Spin at high speeds
• Cell components sort out
  at different layers
  according to density
Microscopic Measurements
• Use the unit called the
  micrometer
• 1/1000 of a meter
How to convert millimeters to
          micrometers
• Multiply by one thousand…or
• Move the decimal point 3 places to
  the right
How to convert micrometers to
          millimeters
• Divide by 1,000…or
• Move the decimal point 3 places to
  the left
• Ex.) 2,500 micrometers =
• .15 micrometers        =
How to find the diameter of your
            field of view
• Place a transparent plastic
  ruler in the field of view like
  this
• 1.3 mm
• Convert to micrometers
• 1.3 mm =
Section 3-2
Prokaryotes and
  Eukaryotes
Eukaryotes
• Have a nucleus and membrane
  covered organelles
Prokaryotes
• Don’t have a nucleus and
  membrane covered organelles
Eukaryotic Cell
  Structures
Plasma Membrane
• Separates the cell from its
  environment
• Controls the transport of materials
  in and out
• Allows some materials but not
  others to pass through this is
  called…
  – Selectively permeable
Endoplasmic Reticulum
• Extensive network of tube-like
  structures that forms a
  passageway that functions in the
  transport of materials throughout
  the cells
Ribosomes
• Site of protein
  synthesis
• Attached to the
  walls of the ER
  or move freely in
  the cytoplasm
Golgi Bodies
• Stack of tiny, flattened sac-like
  tubes used in secretion
• Package protein molecules in a
  membrane and send the package
  to the cells surface
Mitochondria
• Where cellular respiration takes
  place to release energy
• “Mighty Mitochondria”
• Powerhouse of the cell
Microtubules
• Tubes that serve as support for
  the cell
Microfilaments
• Thin threads attached to the cell
  membranes, play a role in
  movement
Lysosomes
• Vesicles that contain enzymes
  used in digestion
• Fuses with food vacuoles to
  digest food into smaller pieces
• Digest old cell structures to
  dispose of them or even entire
  cells
Nucleus
• Regulates all the cells activities
Chromosomes
• Long coiled fibers that carry the
  material of heredity
• Made of protein and DNA
Nucleolus
• Composed of
  RNA
• Involved in the
  passage of
  RNA into the
  cytoplasm
Centrioles
• Small cylinders in the cytoplasm
  that play a role in cell division
Cilia
Flagella
Chloroplasts
• Organelle found only in plants,
  used to make food
Vacuoles
• Spherical, bubble-like storage
  sacs
• Plant cells have very large
  vacuoles compared to animal
  cells
Cell Wall
• Rigid structure       Cell Wall
  that surrounds the
  cell membrane
• Made of cellulose
• Permits most
  things to pass
  through
Section 3-3
Cellular Processes
The Big Idea
• Cells need to regulate the
  movement of dissolved molecules
  on either side of the membrane
Cell membrane
• Regulates what enters and leaves the
  cell and also provides protection and
  support
Outside
of cell
                                            Carbohydrate
                                            chains
                          Proteins
Cell
membrane



 Inside
 of cell        Protein
 (cytoplasm)    channel              Lipid bilayer
Cell membrane
• Made of a double layered sheet called a
  lipid bilayer
Outside
of cell
                                            Carbohydrate
                                            chains
                          Proteins
Cell
membrane



 Inside
 of cell        Protein
 (cytoplasm)    channel              Lipid bilayer
Cell Wall
• Provide support and protection for
  cell
• Found in more than just plants
• Contrary to popular belief, not
  selectively permeable
Cell Walls
Diffusion Through Cell
          Boundaries
• One of the more important
  functions of the cell membrane is
  to regulate the movement of
  dissolved molecules from one
  side of a membrane to the other
Concentration
• The mass of solute in a given
  volume of solution
• Mass/volume
• What is the concentration of 12 grams
  of salt in 3 liters of water?




• What is the concentration of 12 grams
  of salt in 6 liters of water?
In a solution, particles move constantly
Diffusion
• Process by which molecules of a
  substance move from areas of high
  concentration to areas of low
  concentration
Diffusion
Diffusion
Equilibrium
• When the concentration of the
  solute is the same throughout a
  system
• Because diffusion depends on
  random particle movements,
  substances diffuse across
  membranes without energy being
  used
• Even during equilibrium, particles
  still move, but there is no net
  change in concentration
Osmosis
• The diffusion of water
Osmosis
Isotonic
• The concentration of solutes is
  the same inside and outside the
  cell
Hypertonic
• Solution has a higher solute
  concentration than the cell
Hypotonic
• Solution has a lower solute
  concentration than the cell
Facilitated Diffusion
• Movement of specific molecules across
  cell membranes through protein channels
                               Glucose
 •Only go from high   High
                               molecules



   concentrations     Concentration


 to low                  Cell
 concentrations          Membrane



 •Does not require
                      Low
 energy               Concentration
                                           Protein
                                           channel
Types of Active
        Transport
• Sometimes cells must move
  materials in the opposite direction
  – Low concentrations to high
    concentrations
Active Transport
• Energy requiring process that
  moves materials across a cell
  membrane against a
  concentration gradient
Molecule to
                                    be carried


Active Transport


                   Energy



                              Molecule
                            being carried
Molecular transport
• Small molecules and ions are
  actively transported
Carrier proteins
• Proteins that act like pumps
Endocytosis
• The process of taking material into the
  cell by means of infolding of the
  membrane
        Food particle   engulfing   Forms a
                                    vacuole
Phagocytosis
      • Cell eating
      • White blood
        cells do this
Pinocytosis
     • Cell drinking
Exocytosis
• Forces contents out of the cell
• Endocytosis in reverse
Section 3-4
The Diversity of Cellular life
Unicellular Organism
• Single celled
• Do everything a living organism does
Multicellular Organism
• Many celled
• Depend on
  communication
  and cooperation
  among
  specialized cells
Specialization
• When cells develop in different
  ways to perform different tasks
Red Blood Cells
            • Transport
              oxygen
Pancreatic Cells
          • Make
            proteins
          • Packed with
            ribosomes
Skeletal Muscle
       • Generate force
       • Packed with
         mitochondria
       • Overdeveloped
         cytoskeleton
Nerve Cell
     • Send messages
Guard Cells
• Open and close the stomata
Levels of Organization



Cells   Tissues   Organs

                     Organ Systems
Tissues
• Group of similar cells that perform
  specific function
• Ex.) smooth muscle
Organ
• Group of tissues that work
  together to perform a specific
  function
• Ex.) stomach
Organ System
• Group of organs that work together to
  perform a specific function
• Ex.) digestive system

• This organization creates a division of
  labor that makes multicellular life
  possible
Biological organization

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