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Plant Culture
1.
2.
![Plant Tissue Culture ,[object Object],[object Object],Protoplast = actively metabolising part of cell minus cell wall [cell wall digested by enzymes]](https://image.slidesharecdn.com/plant-culture-27654/85/plant-culture-1-320.jpg)
![Process of Plant Cell Culture ,[object Object],2. The cells are then incubated with a mineral salt solution containing mannitol for several hours. This sugar exerts osmotic pressure causing PLASMOLYSIS leading to easier digestion 3. In order for the protoplasts to grow they must be put in a suitable medium to encourage cell wall growth](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)