1. CENTENARIO DE LA CREACIÓN DE LOS ESTUDIOS MICROBIOLÓGICOS
EN LA UNIVERSIDAD NACIONAL DE LA PLATA
 1912 – 2012 
V JORNADAS DE MICROBIOLOGIA CLINICA, INDUSTRIAL Y AMBIENTAL
DE LA PROVINCIA DE BUENOS AIRES
La Plata, Prov. de Buenos Aires 8, 9 y 10 de octubre de 2012
Carrera de Microbiología Clínica e Industrial
Facultad Ciencias Veterinarias. UNLP.
BIOFILMS EN DIFERENTES INDUSTRIAS. ALTERNATIVAS PARA SU
CONTROL
Guiamet Patricia
INIFTA, Departamento de Química, Facultad de Ciencias Exactas, UNLP, CCT La Plata - CONICET. CC
16, Suc. 4 (1900), La Plata, Argentina.
Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET.
pguiamet@inifta.unlp.edu.ar

2. Reuniones relevantes ( + de 70)
■ First Workshop Argentine-USA (CONICET-NSF)
on Biodeterioration of Materials, INIFTA, La Plata,
1984.

3. ■ Dahlem Conference on the Structure and
Functions of Biofilms, Berlin, Germany, 1988.
“Redox/metal dissolution in biofilms”
H.A.Videla.

12. Medio
Productos
Ausencia de O2
corrosivos
Zona
anaeróbica
Cátodo pH Cátodo
Ánodo Metal

15. “Fungal contaminants of jet fuels”. (1995).
H.A.Videla, P.S.Guiamet, E.H.Reinoso.
Biodeterioration & Biodegradation 9 (A. Bousher, N.
Chaudne, R. G. Edyvean, ed.), p. 615-620.
" Activity of isothiozolone biocide against Hormoconis resinae in pure and mixed
biofilms". (1996).
P.S.Guiamet and C.C.Gaylarde.
World Journal Microbiology & Biotechnology, Vol.12, p. 395-397.
" Microbiological Spoilage of Aqueous Based Surface Coatings".
(1996).
P.S.Guiamet and H.A.Videla,
Corrosion Reviews, Vol. 14, (1-2), p.47-57.

16. “Antifouling effects of two satured copper coating applied on carbon
Panel de cobre - Panel testigostructures" (2008) de cobre )
steel (sin cubierta
Guiamet P., Gómez de Saravia S.
Revista Metalurgia 44 (5): 398-405 (ISSN 0034-8570)

17. Understanding biocorrosion in practical cases of
different industrial systems
Patricia Guiamet., Paola Lavin, Mónica Zapponi,
Sandra Gómez de Saravia

18. The aims are a) to present several case studies of MIC found in three plants of extraction and
secondary oil recovery by water injection, due to operating problems. Plants are located in the
provinces of Neuquén (systems 1 and 2) and Mendoza (system 3) b) to suggest possible
solutions for these problems.

19. System 1
• SRB concentration up to 100 times higher than the maximum value of 10 4,
generating then H2S.
• The most important problem was connected with the use of a non oxidizing
biocide (acrolein). It was started with two patching per week of 1100 ppm, each of
them during one hour.
• At the end of 2004 a dispersant plus biocide was used. Pressure increased so
much that serious damages occurred in the system.
• Some 15m3/hr. of fresh water from the Colorado river enter to the water
treatment system into the washing tank. This could be a source of nutrients for the
SRB.

20. System 2
• SRB (1015 mo/mL) were obtained. These high values observed in different
systems are frequently due to staff dismissals caused by socioeconomic problems
or to operators working without the suitable training.
•Tetrakishydroxymethyl phosphonium sulphate (THPS) was applied.
There are high H2S concentrations of genetic and microbiological origin.
•The treatment of water to be reinjected is not very effective.
BRS PIAS
•There are too much solids. 16
14
12
10
cal
do
s 8
6
4
2
0
Ene Feb Mar Abr May Jun Jul Ago
Ent. Planta Sda. Planta Pozo Iny, o Colector

21. System 3
• Blockage of the wells by solids (> 40% per year). The well blockage causes a
significant injection pressure increase.
• There is a generalized problem of corrosion in the valves.
• The processes of the plant are not automatic . It is an operator who regulates the
pumps and do other operations manually, so the smallest periods of concentrations is
enough to cause problems.
• There are high concentrations of sulphides would be deposited on the pipes.
• Biocides used were glutaraldehyde, acrolein and others.

22. ALTERNATIVAS PARA SU CONTROL
“An innovative method for preventing
biocorrosion through microbial adhesion
inhibition”. (1999).
P.S.Guiamet, S.G.Gomez de Saravia and
• Productos Naturales H.A.Videla.
International Biodeterioration &
Biodegradation,43 (1-2), 31-35.
“ The antimicrobial activity of an aqueous extract of
Brassica nigra “. Gómez de Saravia S. G.and Gaylarde
C.C. International Biodeterioration and Biodegradation
41 (1998) 145-148
Cichorium intybusCentaurea cyanus
• Productos Químicos
" Activity of isothiozolone biocide against
Hormoconis resinae in pure and mixed biofilms".
(1996).
P.S.Guiamet and C.C.Gaylarde.
World Journal Microbiology & Biotechnology,
Vol.12, p. 395-397.

23. Global solution proposal for problems of the systems 1, 2 and 3
∗ Service of microbiological management of the systems and a set of
consultancies and specific services.
∗ To collect, organize and display all the information related with microorganisms,
as a tool for monitoring, controlling and preventing the bacteria presence in the oil
systems.
∗ To train the operators and responsible staff of the plants in order to decrease at
the maximum operational mistakes
∗ Theoretical and practical courses on biofouling, biofilms and biocorrosion issues
will be provided to the operators.
∗ Collecting qualitative-quantitative data from planktonic and sessile bacteria
producing biofilms of the systems.
∗ Monitoring through sampling devices
∗ To propose the use of the suitable biocides according to studies performed by
authorized staff.

24. Conclusiones
 Bacterias heterotróficas Potencial riesgo
mesófilas aeróbicas de biocorrosión
 Bacterias productoras de ácido
 Bacterias sulfato reductoras
 Bacterias reductoras de sulfito
Bajos valores de pH Riesgo de
corrosión
inorgánica
 Altas concentraciones de
cloruros y sulfatos en el lixiviado

25. Plan de seguimiento a largo
plazo…
Estudios Estudios
fisicoquímicos microbiológicos
EVITAR PARADAS DE PLANTA,
RECAMBIO DE PIEZAS Y PÉRDIDAS
ECONÓMICAS SIGNIFICATIVAS

26. Equipo
• Dra. Patricia Guiamet
• Dra. Sandra Gómez
de Saravia
• Lic. Patricia Battistoni
• Lic. Paola Lavin