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VIRAL DISCOVERY
CRYSTAL SCOTT
SPRING SEMESTER
GENOME ANNOTATION
BOBB
THE SCALE OF LIFE
http://htwins.net/scale/
THE MILKY WAY GALAXY CONTAINS 300,000,000,000 STARS
THE EARTH CONTAINS 7.5 X 1018 GRAINS OF SAND
THERE ARE 1031 BACTERIOPHAGE ON EARTH
LAST SEMESTER
WHY DOES ONE VIRAL GENOME MATTER?
CURRENTLY IN GENBANK, THERE ARE 103
BACTERIOPHAGE GENOMES
GOALS
PRESENTATION- PHAGEPHEST APRIL
ALL EXPENSE-PAID TRIP
Keynote Speaker:
Martin Chalfie
Nobel Prize 2008
GFP
JANELIA FARM- HOWARD HUGHES MEDICAL INSTITUTE
ANNOTATION IS A PREDICTION
EXPECTATIONS
- You need to be here, every class period
- You need to be engaged
- You need to be METICULOUS about your annotation
- You need to participate in practice annotations, exercises and quizzes
- You need to be actively involved in the final genome annotation file submitted to GenBank
- You need to design two experiments this semester for either Bobb or your phage (may or
may not be able to complete the experiments)
- You need to articulate your work in the form of a poster and an oral presentation by the end
of the semester
HOW?
WE WILL TEACH YOU
THINGS TO REMEMBER
DNA Master guide
Check nucleotide # first thing, and last thing every day
Gene Mark Print out is very, very helpful
Several programs will be used, some intensely, some cursory
YOU will be doing the discovery.
I will be learning from you about Bobb
We will show you everything you need to know to do this novel, amazing bioinformatics on a
previously undescribed bacteriophage
CURRENTLY IN GENBANK, THERE ARE 103
BACTERIOPHAGE GENOMES

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