Session 2: Industrial Biotechnology Title: Replacing Fossil Fuels – How Do We Scale Up Without Compromising On Food And Environment? Dr Jim Flatt, Mascoma

1. Development and Commercialization of Consolidated
BioProcessing for Cellulosic Biofuels Production
MLSCF CEO Life Sciences Conference, 6 October 2009
Jim Flatt, Ph.D.
Mascoma Corporation
CONFIDENTIAL

2. Mascoma Background
Team
• Founded502 by Charlieyman & LeeLyndfrom DartmouthCollege,
W
world renownedleadersin cellulosicbiofuels research
• Experienced industryleadershipteam
• 70 + persontechnologyteam principallybasedin NH
Consolidated Bioprocessing (CBP) Technology
• Gamechanging
“widely consideredto be the ultimate lowrcostconfigurationfor cellulose
hydrolysisandfermentation” – DOE/SA JointResearch
U Agenda
• Enableslowrcostbiologicalconversionof cellulosicbiomassto a broadrangeof products
• Lowcostandabundantfeedstock(currentlytargetinghardwoods)
Facilities
• CorporateOffice& Lab
• Pilot & DemoPlant (Rome,NY)
• Commercial acilityPlanned(inross, Michigan)
F K
• $49.5M in stateandfederal grants

3. Track Record of Securing Significant
Private and Government Funding
• Raised 0
$1 Million of equity
• Strategicinvestmentsby
industryrleadingpartners
• Financial investmentsfrom
toprtier venturecapital funds
• Awardedover $60 million in
FederalandState
Confidential
governmentfunding

4. Why Consolidated Bioprocessing?
lower costs through enzyme reduction and process simplification
Conventional Approach Mascoma Approach
Feedstock
Supply
Steam & Power
Generation
Waste
Treatment Mascoma
Conditioning focus
Enzyme
Hydrolysis
Supply
Conditioning
Fermentation
Key
Developing Distillation
Technology & Storage

5. CBP Organism Development Strategy
Goal
Cellulose Utilization
Ethanol Producer
greenfield retrofit
application application
•C. thermocellum
•T. saccharolyticum Yeast
Substrate Strategy Metabolism Strategy
Cellulose Utilization Commonalities X Cellulose Utilization
X Ethanol Producer Ethanol Producer

6. Mascoma Recombinant Cellulolytic Yeast
March,2007 to December, 2008: >3,000rfold improvement in expression levels

7. Enzyme Reductionon Hardwood
Equivalent
performance with
2.5 rfold less
added enzyme
Further reduction
anticipated

8. Proof of Concept: CBP Yeast Ferments Paper Sludge to
Ethanol with no added cellulase
Positive Control
50 85% Appearance at 120 hrs.
Yield
40
Ethanol Yield (g/L)
CBP
30
20
10 CBP strain
non-CBP
Negative Control strain M1179
0
0 20 40 60 80 100 120 140
Fermentation Time (hours)
18% solids, 1 mg/gTS β

9. CBP Scalerup – Rome Demonstration Facility
60
50
Milestones
Concentration (g/ l)
40
first E85 produced 11/08
30
CBP scale-up Q1.09
20
process engr. 2009 - 10
10
0
0 20 40 60 80 100
Age (h)
120 140 160 180 November 2008
Confidential

10. Promising Initial Results on Palm Residues
Screening Test on Palm Residue Types
• aqueous-based pretreatment
• stem has highest yield
• low lignin (17 – 18%)
Palm Residue Carbohydrate Yield Max Ethanol Yield
(% theoretical) (gal/mt)
Empty Fruit Bunch 73 62
Stem 80 76
Frond 71 62

11. Mascoma Business Model
• Diversify Feedstock
– form funded collaborations to tailor Mascoma technology to other
feedstocks e.g. paper sludge, palm residue and bagasse
– equity participation for both parties
• Diversify End products
– form funded collaborations to adapt Mascoma technology to
produce non-ethanol products
• Commercial Rollout with Regional Partners
– form JV with local partners
– value capture for JV partner and Mascoma via equity
– multiple plant focus
– non-ethanol products

12. Conclusions
– success hardwoodcanbe leveragedto other
on
cellulosicfeedstocks,
includingpalmresidues