Chromatography -procedures and Significance.

1. CHROMATOGRAPHY
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2. Chromatography
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3. Chromatography
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4. Chromatography- Principle
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5. Chromatography- Classification
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6. Chromatography- Classification(Contd.)
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7. Partition Chromatography
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8. Paper chromatography
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9. Paper chromatography
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10. Paper chromatography- Procedure
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11. Paper chromatography- Procedure
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12. Ascending and Descending Paper Chromatography
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13. Paper Chromatography -Analysis
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14. Rƒ value
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15. Two dimensional chromatography
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16. Significance of Paper chromatography
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17. Thin layer chromatography (TLC)
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18. TLC- Procedure
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19. TLC -Overview
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20. Significance of TLC
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21. Advantages of TLC over paper chromatography
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22. 2)Adsorption chromatography
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23. 2)Adsorption chromatography- Elution
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24. Adsorption chromatography
The substance loosely adsorbed to the stationary medium comes in the earlier fraction.
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25. Ion – Exchange chromatography
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26. Ion – Exchange chromatography
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27. Ion – Exchange chromatography- Procedure
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28. Ion – Exchange chromatography-
Procedure (Contd.)
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29. Types of ion exchanges resins
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30. Ion – Exchange chromatography-
An Overview
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31. 4) Gel filtration chromatography or
Molecular Sieve chromatography
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32. Gel filtration chromatography or
Molecular Sieve chromatography
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33. Gel filtration chromatography-An
Overview
Larger particles come out first, while smaller particles come in later fractions
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34. 5) Affinity chromatography
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35. Affinity chromatography- Significance
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36. Affinity chromatography- Significance
By using antibodies, antigens can be easily separated. Conversely,
antibodies can be purified by passing through a column containing the
antigen.
Specific Antibody binds to a specific antigen attached to the stationary medium, the
remaining antibodies come out in earlier fractions. Biochemistry of Medics
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37. 6) High performance liquid chromatography-
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38. High performance liquid chromatography-
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39. High performance liquid chromatography-
Significance
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40. High performance liquid
chromatography- Apparatus
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41. 6) Gas liquid chromatography-
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42. Gas liquid chromatography-
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43. Gas liquid chromatography-Apparatus
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44. Summary
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