by : Ms. Crizelda Liwanag

1. microscopy

2. One of the most widely used tools in
biological sciences
An instrument that produces an
enlarged image of an object
Magnification – the increase in an
object’s apparent size
Resolution – the power of a microscope
to clearly show detail

3. Principle:
The microscope objective first forms in
the tube a magnified aerial image of the
specimen illuminated below the lamp
and the condenser.The image is then
viewed magnified through the eyepiece.
microscope

4. What is the standard length of the draw
tube?
160 mm
Which is the most important
component of the microscope?
Objective lenses
If the image remains visible after the
exchange of objectives, the lenses are
said to be_____
parfocal

6. I. Light microscopes
1. Simple
2. Compound
a. Brightfield
b. Darkfield
c. Phase contrast
d. Ultraviolet
e. Fluorescence
f. Stereoscopic
II. Electron microscopes
1. transmission
2. scanning

7. Type of
microscopy
appearance
magnification
use
Brightfield Microbe
stained
1000x Overall
appearance of
most microbes
Dark field Light
microbe not
stained
1000x Overall
appearance of
most microbes
Phase
contrast
Cellular
component
s resolved
1000x Resolution of
many cellular
components

8. Type of
microscopy
appearance
magnification
use
Ultraviolet UV
absorbing
portion of
cell emitting
light
1000x to
3000x
Cellular
components
that absorb
light
Fluorescent Microbe
fluorescing
1000x to
3000x
m-o’s coated
w/ dye
fluoresce
when struck
by UV

9. Type of
microscopy
Appearance
magnification
use
TEM Ultrathin
section
showing
cellular
component
s (2-D)
Up to
200,000x
Study of
viruses and
detailed
structure of
larger m-o’s
SEM Surface of
microbe
(3-D)
Up to
50,000x
Study of
surface
structure of
m-o’s

10. Brightfield Microscope
Dark image
against a
bright or
white
background

12. Thin, flat components of low
refractile elements and living
cells

13. polarized light
for birefringent substances

14. Brightly illuminated specimen
against a dark background

15. Fluorescent substances
Filters: excitation & barrier

16. Electron beams &
magnetic lenses