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StemRx Bioscience Solutions
Dr. Pradeep Mahajan
Stem Cells Transplant Expert | Founder @ StemRx Bioscience Solutions, India

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You carry your own natural repairing kits in your
own body

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Aging and death do seem nature has planned
for us...
The questions is Can we slow down this
process?
Develop clock of aging Time..
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They fought for “amrut”
…..to become immortal
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"the land of the immortals"
OKINAWA
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“Mankind is always in search of how to prolong life & above
all, to make old age worth living & purposeful…”
Everyone
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wants
to
live
longer
and
look
younger

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Aging ?- How we age
Breakdown in the
cellular safety nets
Functional decline of
molecular, cellular
activities to multiple organ
level
Decrease in the vitality of the
body to adopt various
diseases
Decrease in the reproductivity
Death is the Final event
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Aging / Senesces
Causes of Aging
Geneti
c | DNA
Damag
e
Free
radical
metabolis
m
AGEs
(Advanced
Glycation
End
Products)
Telomer
s
Gene of
Aging
daf2
Gene of
longetivity
 Aging reflects over all body with different pace
 Aging does not occur in same way in every human
being
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Lifestyle, genetic make up has influence on aging

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Organ/ function
Blood
WBC and RBC count decreases
Face / skin
Wrinkles, facial hair, thinning cheek,
double chin
Hair
Graying, thinning, balding
Bones
Loss of bone cells as they become
porous and brittle
Muscles
Loss of muscular mass
Fat
Fat content and distribution change,
storage increases
Joints
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Aging Symptoms
Cartilage becomes cracks and frays

10. Therapy
Cognitive Behavirol Therapy(W C
Bushwell 2006)
www.stemrx.in
What
 Enhancing Self



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
healing capacities
Mobilization of
dormant or sleeping
stem cells
Activate body
immune system
Decreasing Free
radicals
Link between
How
 Diet – Veg & Fruits,





Green Tea
Workout, Meditation,
Breathing Exercises
Coping the stress
Calorie Restrictions
Antioxidants
Growth hormone
supplements

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Average lifespan with Aging diseases
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Death Causes
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So the question is can we
stop aging
An Adult Human once a tiny cell, is
a self assembling machine made of
trillions of microscopic
components…
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Lets see what are the ways
???
Gene Therapy – Narrow Bandwidth
Cell Therapy – Broad Bandwidth
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How to Delay or Reverse
The Aging?
Antiaging
 Remove the damage or Inactive molecules
and cells
 Restore function to several molecules by
repair & Replacement
 Modify the Genetic Program to prevent the
aging process from continuing
How are we going to do Above changes...???
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Who Can take Anti-Aging Treatment
 Absolutely healthy aging (King of Good Times)
 Patients with age related diseases (Struggler
King)
 Aging patients with serious & Advanced diseases
(Bankrupt King)
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 Aging can be reversed by set of cells at a
laboratory level???
Do you believe ?
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Stem cells proportion with aging
How the aging
reflects in this graph?
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growing cells > apoptic cells (Child)
Growing cells = apoptic cells (Mature
person)
Growing Cells < apoptic cells (Aging
Starts)
“Aging has been linked to age
related inabilities of stem cells

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Stem Cells Therapy for
Antiaging
Source : Life Extension Magazine Ju

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What makes given stem cells to
reach to target/damaged site?
 Hypoxia
 SDF1
 HGF
 Platelets
 MSC are magic cells as they release plethora of
natural factors like chemokines and cytokines as
well the microenvironment is equally responsible.
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24. THE ADVANTAGES Of Stem Cell Anti-Aging
Treatment
www.stemrx.in











Stem Cells from your own body are used
No chemicals or drugs are needed
Better breathing and sleeping pattern.
Energy levels restored
Age spots fade
Complexion brightens and skin softens
No more expensive supplements or creams required
Hormones are re-balanced
Sugar levels controlled
Heightened sexual drive
Increased metabolism and surprisingly an average 4-7 kg
weight loss.
 Hair color darkening and volume increasing.
 There is also a better control of blood pressure and improved
vision
 Emotional and cognitive improvement occurs, memory
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improves, concentration ability increases and your ability to

25. Future Enhancements
New Cutting Edge
Technologies
www.stemrx.in
1. VSEL
2. High Telomerase activity cells
3. Therapeutic application of cloned cells
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VSEL
VSEL : Very Small Embryonic/
Epiblast-like stem cells
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Potentially represent the most
regenerative adult stem cells having
embryonic characterization.
Proposed as a viable alternative to
human embryonic stem cells.
They home to site of injuries, upregulate
angiogenesis,
down

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First reported in mouse bone marrow in 2006 by Kucia M et.al
characterized by CXCR4(+)SSEA-1(+)Oct-4+ markers.
Later identified in human blood and bone marrow as well. In 2011
Sovalat H et.al supported the evidence of presence of VSEL in human
bone marrow.
These relevant cells might be issued from very small embryonic-like
stem cells deposited in the BM during ontogenesis and reside lifelong
in the adult BM, and that they could be mobilized into peripheral
blood by granulocyte colony-stimulating factor.
very small embryonic-like stem cells constitute a "mobile" pool of
primitive/pluripotent stem cells that could be released from the BM into
the peripheral blood under the influence of various physiological or
pathological stimuli like injuries, hypoxia, physical exercises etc.
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1.
Studies have shown that VSEL’s are
deposited in adult tissues as backup
population for TCSC
2. An increase in chronic caloric uptake may
prematurely deplete VSELs from adult
tissues, thus contributing to aging. By
contrast, caloric restriction has an opposing
beneficial effect on the tissue-residing pool of
VSELs and longevity.
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Isolation of
VSEL’s from
blood by
aphaeresis
Injection of
purified
VSEL’s in
body
Followed by
GCS factor
mobilization
Characterizat
ion and
enrichment
Mixed with
collagen
sponge
scaffolds
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References
1. Kucia M, Reca R, Campbell FR, Zuba-Surma E, Majka M, Ratajczak J,
Ratajczak MZ, (2006) A population of very small embryonic-like (VSEL)
CXCR4(+)SSEA-1(+)Oct-4+ stem cells identified in adult bone marrow.
Leukemia. May;20(5):857-69.
2. Ratajczak MZ, Zuba-Surma EK, Machalinski B, Ratajczak J, Kucia M.
(2008), Very small embryonic-like (VSEL) stem cells: purification from adult
organs, characterization, and biological significance.Stem Cell Rev.,4(2):8999. doi: 10.1007/s12015-008-9018-0. Epub 2008 May 6.
3. Heider A, Danova-Alt R, Egger D, Cross M, Alt R. (2013),Murine and
human very small embryonic-like cells: a perspective. Cytometry A.;83(1):725. doi: 10.1002/cyto.a.22229. Epub 2012 Nov 16.
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4. Sovalat H, Scrofani M, Eidenschenk A, Pasquet S, Rimelen V, Hénon P
(2011)
Identification and isolation from either adult human bone marrow or G-CSFmobilized peripheral blood of CD34(+)/CD133(+)/CXCR4(+)/ Lin(-)CD45(-)
cells, featuring morphological, molecular, and phenotypic characteristics of
very small embryonic-like (VSEL) stem cells. Exp Hematol. Apr;39(4):495505. doi: 10.1016/j.exphem.2011.01.003.

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5. Aaron M. Havens, Yusuke Shiozawa, Younghun Jung,
Hongli Sun, Jincheng Wang, Samantha McGee, Anjali
Mishra,L. Susan Taichman,Theodora Danciu,Yajuan
Jiang,Gregory
Yavanian,Elizabeth
Leary,Paul
H.
Krebsbach,Denis Rodgerson,and Russell S. Taichman,
Human Very Small Embryonic-Like Cells Generate
Skeletal Structures, In Vivo; STEM CELLS AND
DEVELOPMENT Volume 00, Number 00, 2012 Mary Ann
Liebert, Inc. DOI: 10.1089/scd.2012.0327
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