The document summarizes a 3-month test of APSE TM mosquito control products. Various versions of APSE TM were added to buckets of water at concentrations of 300, 600, and 900 ppm, while untreated control buckets were also used. The buckets were monitored over time to compare larval population levels between treated and untreated buckets. Observations showed that APSE TM products 1 and 2 initially took around 3 weeks to show full effectiveness in controlling larval populations compared to the controls.

1. APSE ™ Mosquito Control Testing
December 15, 2010
The purpose of this evaluation was to test the ability of the APSE ™ chemical to control the
production of mosquito larvae in open containers in a natural outdoor setting, conducive to the
reproduction of mosquitos, over a three month period.
Test Procedure:
Twenty grams of micro-organisms and nutrients were added to each bucket of approximately 2
gallons of water being tested. Three (3) control buckets were left untreated for the duration of
the test. Four different versions of APSE ™ were added to buckets at 300, 600 and 900 ppm.
The buckets were then placed outside 15 feet apart from each other, and allowed to sit open in
hopes of attracting mosquitoes to lay eggs. The buckets were monitored, and the treated
buckets were compared to the control buckets.
In this test of the APSE ™ product line, four (4) different versions of the product were tested at
three different dosages to determine its ability to control the production of mosquito larvae
outdoors. The dosages of APSE ™ in this test were 300, 600 and 900 ppm. In each case each
observation was compared to the control buckets. The duration of the test was three (3)
months. A comparative scale was used to evaluate each container during the course of the
testing. This scale ranged from 1 - 7, with 7 representing the largest population of larvae
observed. The following is a pictorial representation of this chart showing examples of each
larvae population.

2. 0
2
3
4
5
6
7
8
1
0
1
2
3
5
6
7
8
4
9/1/2010
9/1/2010
9/3/2010 9/2/2010
9/3/2010
9/5/2010 9/4/2010
9/5/2010
9/7/2010 9/6/2010
9/9/2010 9/7/2010
9/8/2010
9/11/2010 9/9/2010
9/10/2010
9/13/2010 9/11/2010
Observations:
9/12/2010
9/15/2010 9/13/2010
9/17/2010 9/14/2010
9/15/2010
9/19/2010 9/16/2010
9/17/2010
9/21/2010 9/18/2010
9/23/2010 9/19/2010
9/20/2010
9/25/2010 9/21/2010
9/22/2010
9/27/2010 9/23/2010
September APSE #1
9/24/2010
9/29/2010 9/25/2010
9/26/2010
September Control Samples
9/27/2010
9/28/2010
9/29/2010
9/30/2010
22 (900mls)
21 (600mls)
20 (300mls)
3
2
1
0
1
2
3
5
6
7
8
4
9/1/2010
9/3/2010
9/5/2010
the full benefit of the product was achieved.
9/7/2010
9/9/2010
9/11/2010
9/13/2010
9/15/2010
9/17/2010
9/19/2010
9/21/2010
9/23/2010
9/25/2010
9/27/2010
September APSE #2
9/29/2010
25 (900mls)
24 (600mls)
23 (300mls)
buckets. The results of these observations are displayed graphically below:
During the three months of testing regular observations were made of the test and control
The initial effectiveness of products 1 and 2 appeared to require approximately 3 weeks before

3. 0
1
2
3
4
5
6
7
8
0
1
2
3
4
5
6
7
8
0
1
2
3
4
5
6
7
8
0
1
2
3
4
5
6
7
8
10/1/2010 10/1/2010
11/1/2010 11/1/2010 10/2/2010
10/3/2010 10/3/2010
11/2/2010 11/2/2010 10/4/2010
10/5/2010 10/5/2010
11/3/2010 11/3/2010 10/6/2010
10/7/2010 10/7/2010
11/4/2010 11/4/2010 10/8/2010
10/9/2010 10/9/2010
11/5/2010 11/5/2010
10/10/2010
10/11/2010 10/11/2010
11/6/2010 11/6/2010
10/12/2010
11/7/2010 10/13/2010 10/13/2010
11/7/2010
10/14/2010
11/8/2010 10/15/2010 10/15/2010
11/8/2010
10/16/2010
11/9/2010 10/17/2010 10/17/2010
11/9/2010
10/18/2010
11/10/2010 10/19/2010 10/19/2010
11/10/2010
10/20/2010
11/11/2010 10/21/2010 10/21/2010
October APSE #1
11/11/2010
10/22/2010
10/23/2010
November APSE #1
11/12/2010 11/12/2010 10/23/2010
10/24/2010
October Control Samples
11/13/2010 10/25/2010 10/25/2010
11/13/2010
November Control Samples
10/26/2010
11/14/2010 10/27/2010 10/27/2010
11/14/2010
10/28/2010
11/15/2010 10/29/2010 10/29/2010
11/15/2010
22
21
20
3
2
1
22
21
20
3
2
1
0
1
2
3
4
5
6
7
8
0
1
2
3
4
5
6
7
8
10/1/2010
11/1/2010 10/3/2010
11/2/2010 10/5/2010
11/3/2010 10/7/2010
11/4/2010
10/9/2010
11/5/2010
10/11/2010
11/6/2010
10/13/2010
11/7/2010
10/15/2010
11/8/2010
10/17/2010
11/9/2010
10/19/2010
11/10/2010
10/21/2010
October APSE #2
11/11/2010
10/23/2010
November APSE #2
11/12/2010
10/25/2010
11/13/2010
10/27/2010
11/14/2010
10/29/2010
11/15/2010
25
24
23
25
21
24

4. 0
1
2
3
4
5
6
7
8
0
1
2
3
4
5
6
7
8
0
2
3
4
5
6
7
8
0
1
2
3
5
6
7
8
1
4
10/1/2010 10/1/2010
10/2/2010 9/1/2010 9/1/2010
10/3/2010 10/3/2010 9/2/2010
10/4/2010 9/3/2010 9/3/2010
10/5/2010 10/5/2010 9/5/2010 9/4/2010
10/6/2010 9/5/2010
10/7/2010 10/7/2010 9/7/2010 9/6/2010
10/8/2010 9/7/2010
10/9/2010 9/9/2010 9/8/2010
10/9/2010
10/10/2010 9/11/2010 9/9/2010
10/11/2010 9/10/2010
10/11/2010 9/11/2010
10/12/2010 9/13/2010
10/13/2010 9/12/2010
10/13/2010 9/15/2010 9/13/2010
10/14/2010 9/14/2010
10/15/2010 10/15/2010 9/17/2010 9/15/2010
10/16/2010 9/19/2010 9/16/2010
10/17/2010 10/17/2010 9/17/2010
10/18/2010 9/21/2010 9/18/2010
10/19/2010 10/19/2010 9/19/2010
9/23/2010 9/20/2010
10/20/2010
10/21/2010 10/21/2010 9/25/2010 9/21/2010
October APSE #3
10/22/2010 9/22/2010
10/23/2010 9/27/2010 9/23/2010
September APSE #3
10/23/2010 9/24/2010
10/24/2010 9/29/2010
October Control Samples
10/25/2010 9/25/2010
10/25/2010 9/26/2010
September Control Samples
10/26/2010 9/27/2010
10/27/2010 10/27/2010 9/28/2010
10/28/2010 9/29/2010
10/29/2010
10/29/2010 9/30/2010
28 (900mls)
27 (600mls)
26 (300mls)
28
27
26
3
2
1
3
2
1
0
1
2
3
4
5
6
7
8
0
1
2
3
5
6
7
8
4
10/1/2010 9/1/2010
10/3/2010 9/3/2010
9/5/2010
10/5/2010
the full benefit of the product was achieved.
9/7/2010
10/7/2010 9/9/2010
10/9/2010 9/11/2010
10/11/2010 9/13/2010
9/15/2010
10/13/2010
9/17/2010
10/15/2010
9/19/2010
10/17/2010 9/21/2010
10/19/2010 9/23/2010
10/21/2010 October APSE #4 9/25/2010
9/27/2010
September APSE #4
10/23/2010
9/29/2010
10/25/2010
10/27/2010
10/29/2010
31 (900mls)
30 (600mls)
29 (300mls)
31
30
29
The initial effectiveness of products 3 and 4 appeared to require approximately 3 weeks before

5. November Control Samples November APSE #4
8 8
7 7
6 6
5 5
4 4
1 29
3 2 3 30
2 3 2 31
1 1
0 0
11/1/2010
11/2/2010
11/3/2010
11/4/2010
11/5/2010
11/6/2010
11/7/2010
11/8/2010
11/9/2010
11/10/2010
11/11/2010
11/12/2010
11/13/2010
11/14/2010
11/15/2010
11/1/2010
11/2/2010
11/3/2010
11/4/2010
11/5/2010
11/6/2010
11/7/2010
11/8/2010
11/9/2010
11/10/2010
11/11/2010
11/12/2010
11/13/2010
11/14/2010
11/15/2010
November APSE #3
8
7
6
5
4
26
3 27
2 28
1
0
11/10/2010
11/11/2010
11/12/2010
11/13/2010
11/14/2010
11/15/2010
11/1/2010
11/2/2010
11/3/2010
11/4/2010
11/5/2010
11/6/2010
11/7/2010
11/8/2010
11/9/2010
There did not appear to be a marked difference in the performance of the different versions of
APSE ™ . The overall results were almost identical for each product version and dosage.
Conclusion:
APSE ™ reduced the overall production of mosquito larvae by over 60% when compared to the
controls in this experiment at all dosage ranges and product strengths. The product is
extremely effective in the control of mosquito reproduction. These test results are deemed to
be conclusive and reproducible based on Standard Methods.
Dustin Smith
Chief-Microbiologist
HML, Inc.
912 W. McGalliard
Muncie, IN 47303
765-288-1124