This small subpopulation of cells are suspected to be the drivers of tissue repair in vivo. This makes them key to stem cell based drug discovery and other regenerative medicine applications. Characteristics include: gene expression patterns associated with the down-regulation of genes involved in cell death and survival; high expression of homing/migration chemokines.

1. StressedSelected
Human Mesenchymal
StemCells
Potent, Pure and Easy to Culture

2. What makes
these cells
unique?
New Stress Select Human Stem Cells-They
Survive toThrive
Capabilities:
•High potency, expansion rates and passaging.
•Low telomerase activity=low tumorigenesis
properties
•High ability to migrate and differentiate to
specific tissue types-High expression of
migration/homing chemokines

3. Image:We testedtheeffects ofcellular
stressonhumanCB-MSCs(Neuromics’,
SC00A1) sinceothershavereported
conversiontothepluripotentstate
(Heneidi,S.,et al.,PLoSOne8:e64752,
2013). We recovered humanCB-MSCs
fromcryopreservationandbriefly
culturedthesecells (48hours)at20%
O2-5% CO2inMSC-Gro,low-serum
medium(Neuromics, SC00B1).Asa
stressfulenvironment,thesecells were
thentransferred to1% O2-5%CO2at
105/ml innutrient-poormedium
(expiredRPMI)supplementedwith
0.12PZ/mlcollagenaseNB4(Serva,
catalognumber17454) at4oC. We
retrieved viablecells 48-hourslaterand
transferredthemtoMSC-Gro
humanized,completemedium
(Neuromics’,SC00B2).Theimages
aboveshowformationofmulti-cell
clusters~50 to130 umat2days(Left)
and4days(Rt)followingexposureto
stress. Scalebaris50mm.

4. Image: Further
morphology of these
cultures is shown
above as phase
contrast images of two
prominent clusters
together with more
firmly attached cells of
classical mesenchymal
morphology. This is
day seven following
exposure to stress.
Scale bar is 50 um.

5. Images: (A) Clusters of stress-
selected cells are positive for
SSEA-3, a pluripotency marker.
Following blockage of non-
specific binding, cultures were
incubated with a Alexa Fluor-
conjugated SSEA-3 monoclonal
antibody (MC-631-AF 488;
BioVision, catalog number
3068-25T) at 10 ml/ml
overnight. (B)SSEA-3 positive
cells within cell clusters
together with propidium
iodide. Red cells are non-
viable. SSEA-3 is specific to
embryonic stem cells; MSCs
were negative for SSEA-3.

6. Stress Selected
Stem Cells
Introductory
offer-Save 150
USD
Ordering Options
Order online @ Stress Selected
Stem Cells
•Call: 1-866-350-1500 (international:
952-374-6161)
•Fax: 612-677-3976
•E-mail: please email
brett@neuromics.com
600 USD for a vial of
500,000 cells.