BioVale Energia - results achieved with its research for Jatropha-curcas in partnership with UFV and Fapemig

1. Jatropha-curcas:
Innovative R&D project

2. Business focus
Biovale Energia
2
Agronomy, Science
Sustainable plantation, social inclusion
for Oils Supply Program
Collection of accessions – Germoplasm
collection
FMA for productivity and plant health
Jatropha detoxification process
Mushroon production
Industry
Jatropha planting to crude oil
Planting, maintenance and
harvesting
Expelling, preprocessing and
logistics
Crude oil sales
www.jatrophanetwork.ning.com|biovaleminas@gmail.com

3. Mycorrhizal germplasm reservoir of genetic diversity of mycorrhizal fungi ofMycorrhizal germplasm reservoir of genetic diversity of mycorrhizal fungi of
jatropha-curcas with high oil yielding for biodiesel production
Germoplasm collection of jatropha accessions
Elimination of the toxicity of the meal]waste for livestock feed
Executing organization :
R&D goals and stakeholders
BIOVALE ENERGIA – an emerging consulting biodiesel company based in the
Minas Gerais State.
Co-executing organizations:
UFV -Federal University of Viçosa – DMB/BIOAGRO, benchmark in bio sciences
SECTES|FAPEMIG – Minas Gerais State Science and Technology Secretariat
EPAMIG – EMPRESA DE PESQUISA DE AGROPECUÁRIA DE MINAS, national bench-
mark in jatropha development
CETEC - CENTRO TECNOLÓGICO DE MINAS GERAIS - CETEC

4. “Take joint actions and improve efforts to work together at
all levels to improve access to reliable and affordable energy
services for sustainable development sufficient to facilitate
the achievement of the MDGs, including the Goal of halving
the proportion of people in poverty by 2015, and as a means
to generate other important services that mitigate poverty,
bearing in mind that
visionvisionvisionvision
bearing in mind that
access to energy facilitates the eradication of poverty”
( Summit on Sustainable Development in the
Johannesburg Plan of Implementation )
“ We folks have to get out of the dry hinterland! But one only gets out
of the dry hinterland is taking it over from inside...” (Guimarães Rosa)

5. As developing countries face increasing local demand for energy in rural
areas, they also must deal with both economic and environmental
pressure on agricultural lands in general.
Growing energy crops such as Jatropha curcas L. has the potential to
enable some smallholder farmers to cope with these pressures.
challange
The partnerships
should be at the
global level, at theglobal level, at the
country level with
national
stakeholders and
external partners
acting together, the
private sector and
civil-society
institutions
collaborating to
create conditions
that emancipate
poor groups.
But the fundamental partnership, and ultimately
the only one that counts, is with the poor
themselves. They have the talents, the skills and
the knowledge of their own environment.

6. Jatropha is an underutilized, oil-bearing crop. It produces a seed that
can be processed into non-polluting biodiesel that, if well exploited,
can provide opportunities for good returns and rural development
Almost 2.5 billion people in developing countries earn their livelihoods
from agriculture. Of these, 900 million live below the poverty line of
USD 1.00 a day. In addition, agriculture directly employs 1.3 billion
people, or 40 percent of the global labour force, yet agriculture only
potential
people, or 40 percent of the global labour force, yet agriculture only
contributes around 4 percent of global GDP (some USD 1.6 trillion).
This underscores the importance of biofuels in alleviating poverty.
In 2008, jatropha was planted on an estimated 900 000 ha globally – 760 000
ha (85 percent) in Asia, followed by Africa with 120 000 ha and Latin America
with 20 000 ha. By 2015, forecasts suggest that jatropha will be planted on
12.8 million ha. Brazil will be the largest producer in Latin America. (UN)

7. Biofuel production
has become
substantially more
efficient over the last
25 years as Brazil and
the United States
have scaled up their
industries.
New Technologies, New Gains
have scaled up their
industries.
Such incremental
gains are likely to
continue for years to
come.
However, the greatest potential for biofuels lies in theHowever, the greatest potential for biofuels lies in the
development of new technologies that will significantly
expand the range of biomass feedstock, increase
conversion efficiencies, and lower production costs.

8. Jatropha has been identified as one the best plantJatropha has been identified as one the best plant
alternative to offer clean fuel for achieving energy
security. Jatropha seeds inoculated with in vitro-raised
mycorrhiza exhibits early fruition and flowers from
the 7th month onwards as against a year with
conventional clonal plantations and two years from
seed raised plantations.
More productivity
The mycorrhized Jatropha also exhibited 20%–30%
higher yields as compared to non-mycorrhizal
plantations.
The mycorrhized Jatropha will be widely tested
covering several agro-climatic regions in Brazil to
prove their adaptation in diverse soils.

9. The ROC (root organ culture) system is the most
attractive and advanced cultivation methodology for
AM (arbuscular mycorrhizal ) fungi: it uses root-
inducing transfer-DNA-transformed roots of a host
Mass inoculum technology
inducing transfer-DNA-transformed roots of a host
plant to develop the symbiosis on a specific medium in
vitro which provides pure, viable, contamination-free
inoculum using reduced space.
BioVale R&D Project aims at gaining expertise in ROC
for in vitro culture and utilize mass production of AM
fungi under in vitro.

10. Documentation, preservation and characterization of J.
curcas and mycorrhizal fungi germplasm
Development of micropropagated plants inoculated of
ROC (Root Organ Culture) of arbuscular mycorrhizal (AM)
fungi and their in vitro mass inoculum production
Expected results
ROC (Root Organ Culture) of arbuscular mycorrhizal (AM)
fungi and their in vitro mass inoculum production
Selection of jatropha genotypes with high oil yielding,
envisaging the biodiesel production
Toxicity elimination of the waste for animal feed
Utilization and reclamation of industry created wastelands

11. It exists a wide diversity within the AM fungi which form association
with the roots of almost 80% plant species and enhance mineral
nutrient acquisition and water uptake, as well as increase tolerance
towards different environmental stresses conditions.
To conserve and exploit their diversity, BioVale Project contemplates
to build J. curcas and mycorrhizal fungi culture depository houses and
maintain cultures from different agro-ecological zones, in different
Results achievedResults achievedResults achievedResults achieved
maintain cultures from different agro-ecological zones, in different
areas of Brazil.
Three localities were selected: Florestal, Viçosa and Janaúba.
The expected result is to trap isolate a variety of J. curcas and
polysporal/monosporal cultures, setting up a J. curcas and mycorrhizal
fungi germplasm collection of genetic diversity of agriculturally and
industrially species for oil production . 46 accessions were
characterized.
We identified19 FMA especies in the rizosphere of jatropha.

12. One of the main challenges that mankind will be facing during this century
will be the necessity to drastically increase animal production levels and
productivity in order to fulfill the feeding demands of a growing world
population.
J. curcas is a multipurpose tree of significant economic importance because
of its several industrial and medical uses. Jatropha grows throughout most of
the tropics. It survives on poor stony soils and is resistant to drought, reaches
a height from 3 m to 8 m and can be used to reclaim land. The seed weighs
Jatropha challenge
a height from 3 m to 8 m and can be used to reclaim land. The seed weighs
approximately from 0.53 -0.86 g and its kernel contains 22 -27 % protein and
57 -63 % lipid indicating good nutritional value.
The oil from these seeds can serve as fuel for diese! engines, indicating its
potential as a renewable energy source . The potential impact is immense for
countries with no indigenous fossil fuel or for regions remote from a source of
supply. Furthermore, use of biodiescl is friendly to the environment.
However, the seed and oil have been found to be toxic to. Hence, its use as a
food or feed source is presently limited.

13. Most of the physical and chemical treatments being proposed so far
have not been able to definitively decrease the levels of these toxic
compounds.
Treatment of J. curcas seed cake by solid-state incubation with white-
rot fungi is able to promote the decrease of phorbol esters content to
non-toxic levels, showing that this might be an alternative treatment
Detoxification routes
non-toxic levels, showing that this might be an alternative treatment
with future potential.
White-rot fungal basidiomycetes are known for their ability to degrade
a wide range of xenobiotics, such as polycyclic aromatic hydrocarbons,
polychlorinated biphenyls and synthetic dyes due to their enzymatic
systems.
The development of a biological approach to treat J. curcas seed cake
is an environmentally sound and a more economical process.

14. Enzymatic complexes produced by white-rot fungi have also an
enormous potential to be used in the treatment of fibrous feedstuffs
improving its nutritive value through the sequential degradation of
certain refractory cell wall components .
The selected white rot fungi used for the research showed great
biological productivity and efficiency for the production of edible
Results achievedResults achievedResults achievedResults achieved
biological productivity and efficiency for the production of edible
mushrooms grown in the jatropha-curcas cake, adding more value to
the chain.
END RESULT: after assessing the nutritional value and the security for feeding
Livestock (caprines) in different levels of itake in the growth diet, the results
obtained from the treatment with white-rot fungi eliminated anti-nutritional
compounds and is recommended to use the treated cake up to 20 % intake
PATENT DEPOSITED AT THE BRAZILIAN PROPRIETARY INDUSTRIAL REGISTRY

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Setting up a prototype at the University for industrial scale upSetting up a prototype at the University for industrial scale upSetting up a prototype at the University for industrial scale upSetting up a prototype at the University for industrial scale up