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HOW PRIOR KNOWLEDGE AND COLOUR CONTRAST
INTERFERE VISUAL SEARCH PROCESSES IN NOVICE
      LEARNERS: AN EYE TRACKING STUDY




                                                 CELDA 2012




Hacettepe University / TÜRKİYE
Duygu Sönmez, Arif Altun & Sacide Güzin Mazman
Introduction
   In science education, use of                The success of information
   visual materials to support                 transfer is increases
   verbal communication                        especially for abstract
                                               concepts


       Diagrams,
       photographs
       illustrations,
       models photographs,
       simulations,
       microscope,
       telescope
       ……………..


Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                           CELDA 2012
Introduction

                         Biology education        Astronomy Education.




                              Microscope                     Telescope



                                     provide visual information
                                      for materials that are not
                                         visible to naked eye




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                             CELDA 2012
Introduction


                                     Recognition and interpretation
                                           of visual materials




                                 visual literacy and visual search
                                 strategies emerge as the vital skills
                                            at individual level




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                            CELDA 2012
Visual Search

     Visual search tasks are commonly used both as a tool and a method to
      investigate how attention influences visual information processing, namely how
      the visual system extracts and combines.


     Factors that increase or decrease efficiency of visual search could be
      categorized into image driven factors (i.e. saliency, single features, colour
      contrast, abrupt onset luminance contrast) and knowledge based factors (i.e.
      familiarity effect, novelty, experience, automaticity, pre-knowledge)




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                  CELDA 2012
Visual Search

      In mainstream K-16 education, use of visual materials relies on the successful
      visual search strategies in the learning process as well as many professions.
      In radiology, histology, pathology, medicine, science or meteorology;
      recognition and interpretation of visual information is necessary for diagnostic
      purposes.

     In biology education, microscopes are the tools that are frequently used in
      grades K-16 to provide visual information to students on content and materials
      that are too small for naked eye such as blood, cheek cells or cells that are
      going through mitosis.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                   CELDA 2012
Visual Search

     Students are assumed to have the necessary skills to be able to conduct
      successful visual search when looking through microscope binoculars and
      their success in interpretation of an image is rarely questioned or assessed
      in most cases.
     Existing research focuses on visual search with the purpose of identifying
      specific patterns to support the learning process.
     Studies conducted with the individuals from different professions shows
      differences in visual search performance generally based on individual’s
      expertise level, prior exposure and visual display design characteristics.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                      CELDA 2012
Visual Search


     Experts in various fields learn to recognize specific patterns and make
      connections to the prior knowledge through perception

     Understanding students’ interpretation of microscope slides in relation to
      their level of content knowledge and their expertise level, which is
      developed through repetition, holds a merit for the future use of such visual
      materials in education.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                  CELDA 2012
Eye Movements in the Visual Search Process

     Eyes provide a window into mental life.
     Eye movements, as on-line measures of cognitive processes in
      general, contain amount of information that reaction time lack by
      continuously track both in space and time the evolution of mental
      events.
     Human visual attention and eye movements are in a close correlation
      and analysing eye movements would provide objective measures of
      common attentional processes (Hoffman and Subramaniam 1995; Frey
      et al. 2011).
     Eye movement metrics provide to understand the cognitive processes
      and to determine individuals’ cognitive profiles as to whether they are
      poor or good at some cognitive abilites.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                   CELDA 2012
Eye Movements in the Visual Search Process

       Eye-tracking technology is also suggested as a “method for analysing
        learners’ interactions with different forms of representations in
        multimedia learning” (Chuang and Liu 2011, p. 1).
       Since eye movement data can provide information on how users receive
        and process visual information, general trend in literature has been to
        identify differences among novice and experts’ performance with field
        related visual materials through eye metrics data.
       Eye movement studies investigating performance among various
        expertise levels indicates that experts perform visual search with fewer
        saccadic movements with more accuracy in comparison to less trained
        participants (Krupinski et al. 2006; Leung et al. 2007).
       In addition it has been stated that prior knowledge has a significant role
        on eye metrics such as fixation.



Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                        CELDA 2012
Significance of Study

      Understanding students’ interpretation of microscope slides in relation to
       their level of content knowledge and their expertise level, which is
       developed through repetition, holds a merit for the future use of such visual
       materials in education.

      Moreover, understanding the effect of prior knowledge and prior exposure
       with or without a well-designed environment is also important in terms of
       science education.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                   CELDA 2012
Purpose of the Study

        Shedding light on how students conduct visual search and interpret
         microscope slides in relation to their level of pre-existing content knowledge
         can help in the use and development of visual aids to support learning process
         in education.

        Therefore, the purpose of this study is to target novice individuals who are
         lack of repetition and expertise in interpreting microscope slides and to
         investigate their visual search processes.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                    CELDA 2012
Research Questions
    Do the eye movements during the visual search task in different background
      colour contrast differ in terms of individual’s prior knowledge level? (high /
      low color contrast)?
           Do the eye movements during the visual search task in high colour contrast
             images differ in terms of individual’s prior knowledge level?
           Do the eye movements during the visual search task in low colour contrast
            images differ in terms of individual’s prior knowledge level?
     Do the eye movements during the visual search task in different background
      colour contrast differ in terms of having prior exposure to microscope view?
          Do the eye movements during the visual search task in high colour contrast images
           differ in terms of having prior exposure to microscope view?
          Do the eye movements during the visual search task in low colour contrast     not
           images differ in terms of having prior exposure to microscope view?
     Is the interaction effect between prior knowledge and prior exposure
      significant?


Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                             CELDA 2012
METHOD
Study Group

      Prior content knowledge test was administered a total of ninety-eight, 3rd
       and 4th year undergraduate students .
      All participants were pre-service elementary science teachers who
       completed at least two general biology courses and had the basic skills of
       microscope use.
      Participants were grouped based on their prior content knowledge levels on
       the phases of mitosis as high or low level groups.
      40 students were appointed to either high or low level groups, 20 students
       to each group.
      For the purpose of this study, all participants were novices in terms of their
       experience of using microscopes.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                       CELDA 2012
Demographic characteristics

      Demographic Characteristics                  F    Percentage (%)

                                          Female   28   70%
      Gender
                                          Male     12   30%

                                          3        28   70%
      Grade
                                          4        12   30%

                                          Low      20   50%
      Prior Knowledge
                                          High     20   50%

                                          Yes      20   50%
      Prior exposure to microscope view
                                          No       20   50%
Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                     CELDA 2012
Data Collection Tools
  The prior knowledge Test: This content test consisted of 10 multiple choice
  questions which was developed by a field expert who is also one of the researchers.
  The item discrimination indices for the questions ranged between 0.32 and 0.66. KR-
  20 reliability coefficient was found to be .717.
  Each correct answer was scored 1 point and wrong answer was scored 0 point.
  Visual Search Questions: Seven different microscope images of cells were
  displayed on a computer for each question. Question images were grouped in two
  sets; high and low contrast. The first set included four questions which were high
  contrast images with maroon coloured genetic material on a light blue cell . Second
  set included three low contrast slides with orange colored genetic material on a
  yellow cell background.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                      CELDA 2012
Experimental Setting


       All of the cell images were transformed to an experiment set by Tobii
        software on eye tracker.
       Tobii T120 Eye tracker was used to record eye movement data by
        two binocular infra-red cameras integrated within the panels of the
        monitor.
       Fixation duration and time to first fixation were obtained from eye
        tracker for this study.
       Undergoing mitosis phases on the cells were drawn as area of
        interests. Those area of interests were the areas which students
        required to find and recognize the right mitosis phases.
       Participants’ eye metrics data were recorded during the process and
        eye movement data on those areas were collected to analyse.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                   CELDA 2012
Area of interest samples from different background colour contrasts questions




   Participants were asked to find the cell that is undergoing through any mitotic
    phase and identify the cell by clicking on it with the curser and then to select
    the name of the mitotic phase among multiple choices presented on the right
    side of the screen.
   Each of the questions was displayed 20 seconds on the screen.

Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                   CELDA 2012
Data Analysis

      A 2x2 within subject design is used for the purpose of this study.



      Participants were grouped by their level of prior content knowledge
       (high and low) and their statement on either having a prior
       exposure to slides showing phases of mitotic division with the
       consideration of priming effect.



      Dependent variables: Time to first fixation, fixation duration




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                CELDA 2012
FINDINGS
Eye movement statistics across prior knowledge and prior exposure groups

                                   Low level prior knowledge         High level prior knowledge
                               Prior exposure   No Prior exposure Prior exposure    No Prior
                                   (n=10)            (n=10)           (n=10)     exposure (n=10)
                          M          1.46             1.29             2.79            2.81
            Fix.Dur.
                          Sd         .806             .764                             1.94
   Blue




                                                                       1.27
                          M          7.04             7.52             4.66            3.98
            T_FirstFix.
                          Sd          5.2             5.07             3.61            4.33
                          M          .818             .946             1.28            .889
            Fix.Dur.
   Yellow




                          Sd         .728             .813             .852            .697
                          M          4.93             3.52             3.84            5.18
            T_FirstFix
                          Sd         3.24             2.87             2.31            5.22

    Descriptive analyses indicate that for high contrast images, students with higher
  content knowledge (a) fixate on the cells faster in comparison to students with lower
  content knowledge and (b) have a longer fixation period of time on the images. For
  further analysis, a two-factor MANOVA was conducted.

Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                                     CELDA 2012
MANOVA Results

    The main effect of prior knowledge was found significant (Wilk’s Λ = . 677),
     F(4, 33) = 3.932, p =.010, ηp2 = .323).

    Neither the main effect of prior exposure to microscope view (Wilk’s Λ =. 989),
     F(4, 33) = 0.91, p = .985, ηp2 = .011) nor the interaction effect of prior
     knowledge and prior exposure to microscope view (Wilk’s Λ = . 880),
     F(4, 33) = 1.129, p =.369, ηp2 = .120) were found significant.

    High level prior knowledge group was fixated on the area of interests (time to
     first fixation) significantly in a shorter time than low level prior knowledge group
     in blue background questions (high contrast); F(1, 36)=4.14, p=.049, ηp2 = .103.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                     CELDA 2012
MANOVA Results


      In the blue background (high contrast) questions, total fixation duration of
       high level prior knowledge group was found to be higher than their low level
       counterparts F(1, 36)=12,183, p=.001, ηp2 = .253.

      However, in yellow background (low contrast) questions, no statistically
       significant difference between prior knowledge level groups was found
       neither in time to first fixation nor in total fixation duration in the area of
       interests.




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                            CELDA 2012
DISCUSSION

     This study investigated the effect of prior knowledge and prior exposure in
      different colour contrasted microscope images.
     The images used as questions in the study were designed in two different
      colour contrasts, high contrast; maroon targets on blue background versus
      low contrast; orange targets on yellow background, to examine whether the
      colour contrast facilitate the recognition process in visual search tasks.
     The findings revealed out that for high colour contrast image group prior
      knowledge has an effect in visual search tasks both in time to first fixation,
      which shows recognition, and fixation duration on the related areas.

    This finding is consistent with individual differences principle of Mayer (2001) in the
    multimedia design, which states that prior knowledge has a compensating effect
    on producing own mental models in multimedia learning (Mayer &Moreno 1998).


Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                      CELDA 2012
DISCUSSION

     •   In high colour contrast questions, high level prior knowledge group
         recognized area of interest in a shorter time and fixated on correct areas
         longer than other areas.


     This finding is also consistent with prior research which reports that combinations
     of colours that had higher levels of contrast between background and foreground
     and also higher prior knowledge generally led to better performance than
     combinations of lower (Ling and Schaik 2002; Theeuwes, Reimann and Mortier
     2006; Huang 2008; Zhuang and Papathomas 2011).




Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                     CELDA 2012
DISCUSSION

      On the other hand, visual performance of high level prior knowledge group
       and low level prior knowledge group was found to be similar on low contrast
       images.
      Nevertheless, based on the findings of this study it is difficult to suggest that
       prior knowledge effect has disappeared in low contrast background
       microscope images.
      It should be noted that time to first fixation occurs in a shorter period of time,
       and the difference is greater for low level prior knowledge group with no
       prior exposure between high and low contrast pictures.
      Follow up to explore this difference, data were revisited and the length of
       first fixation duration metric results were also computed in order to explore
       whether these first fixations would be taken as an evidence of visual search
       or a mere scanning process.



Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                       CELDA 2012
DISCUSSION

      The length of first fixation durations were shorter in low contrast pictures.
      The low contrast pictures had a quicker fixation but less time spent whereas
       those studying the high contrast pictures had relatively fixated later but
       stayed longer on the areas of interests.
      It can be speculated that their first fixation could just be interpreted as an
       indication of mere eye scanning on the picture.
    Since visual search and scanning are different cognitive processes, additional data
    is required to be able to predict if the prior knowledge affect disappeared or not.

      Furthermore, fixation duration is stated to being an indicator of the cognitive
       complexity (Henderson and Hollingworth 1998).
    Based on the findings in this study, it can be speculated that low contrast images
    might increase the cognitive complexity for participants.


Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                       CELDA 2012
CONCLUSION

     In conclusion, the role of visual representations cannot be ignored in
        science education.
     This study investigated visual search process by the measurement of eye
        movements which is an objective measurement of cognitive processes. The
        results revealed that for novices, who are lack of expertise in interpreting
        microscope images, such as participants of this study, prior knowledge has
        an effect when high contrast images were in question.
     Findings of this study can be useful for both teachers and instructional
        designers in science education. Instructional designers should consider
        design principles together with individual differences when developing visual
        displays.
     Teachers also should consider individuals’ abilities and properties of visual
        materials when choosing instructional materials.


Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                    CELDA 2012
CONCLUSION

        Individual differences (i.e. visual ability, prior knowledge, visual attention,
         working memory, visual perception) are important in understanding the
         visual materials and also in learning from multimedia environments.
        However, effect of individual differences could be sensitive to the design of
         the learning environment.
        Although people could benefit from their positive individual characteristics
         in well-designed learning environments, ever so novel or familiar, increase
         in the complexity of material would diminish the advantage of previous
         knowledge.
        Reversely, well designed display designs found to be facilitating
         comprehension of students who has low prior knowledge
        Further research could explore the effects of other individual differences
         such as gender, age, and distinctive socio-cognitive characteristics on
         visual processes in multimedia environments.

Sönmez, D, Altun, A.& Mazman, S.G.
Hacettepe University/ TURKIYE                                                       CELDA 2012
Visual search sonmez_altun_mazman

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Visual search sonmez_altun_mazman

  • 1. HOW PRIOR KNOWLEDGE AND COLOUR CONTRAST INTERFERE VISUAL SEARCH PROCESSES IN NOVICE LEARNERS: AN EYE TRACKING STUDY CELDA 2012 Hacettepe University / TÜRKİYE Duygu Sönmez, Arif Altun & Sacide Güzin Mazman
  • 2. Introduction In science education, use of The success of information visual materials to support transfer is increases verbal communication especially for abstract concepts Diagrams, photographs illustrations, models photographs, simulations, microscope, telescope …………….. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 3. Introduction Biology education Astronomy Education. Microscope Telescope provide visual information for materials that are not visible to naked eye Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 4. Introduction Recognition and interpretation of visual materials visual literacy and visual search strategies emerge as the vital skills at individual level Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 5. Visual Search  Visual search tasks are commonly used both as a tool and a method to investigate how attention influences visual information processing, namely how the visual system extracts and combines.  Factors that increase or decrease efficiency of visual search could be categorized into image driven factors (i.e. saliency, single features, colour contrast, abrupt onset luminance contrast) and knowledge based factors (i.e. familiarity effect, novelty, experience, automaticity, pre-knowledge) Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 6. Visual Search  In mainstream K-16 education, use of visual materials relies on the successful visual search strategies in the learning process as well as many professions. In radiology, histology, pathology, medicine, science or meteorology; recognition and interpretation of visual information is necessary for diagnostic purposes. In biology education, microscopes are the tools that are frequently used in grades K-16 to provide visual information to students on content and materials that are too small for naked eye such as blood, cheek cells or cells that are going through mitosis. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 7. Visual Search  Students are assumed to have the necessary skills to be able to conduct successful visual search when looking through microscope binoculars and their success in interpretation of an image is rarely questioned or assessed in most cases.  Existing research focuses on visual search with the purpose of identifying specific patterns to support the learning process.  Studies conducted with the individuals from different professions shows differences in visual search performance generally based on individual’s expertise level, prior exposure and visual display design characteristics. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 8. Visual Search  Experts in various fields learn to recognize specific patterns and make connections to the prior knowledge through perception  Understanding students’ interpretation of microscope slides in relation to their level of content knowledge and their expertise level, which is developed through repetition, holds a merit for the future use of such visual materials in education. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 9. Eye Movements in the Visual Search Process  Eyes provide a window into mental life.  Eye movements, as on-line measures of cognitive processes in general, contain amount of information that reaction time lack by continuously track both in space and time the evolution of mental events.  Human visual attention and eye movements are in a close correlation and analysing eye movements would provide objective measures of common attentional processes (Hoffman and Subramaniam 1995; Frey et al. 2011).  Eye movement metrics provide to understand the cognitive processes and to determine individuals’ cognitive profiles as to whether they are poor or good at some cognitive abilites. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 10. Eye Movements in the Visual Search Process  Eye-tracking technology is also suggested as a “method for analysing learners’ interactions with different forms of representations in multimedia learning” (Chuang and Liu 2011, p. 1).  Since eye movement data can provide information on how users receive and process visual information, general trend in literature has been to identify differences among novice and experts’ performance with field related visual materials through eye metrics data.  Eye movement studies investigating performance among various expertise levels indicates that experts perform visual search with fewer saccadic movements with more accuracy in comparison to less trained participants (Krupinski et al. 2006; Leung et al. 2007).  In addition it has been stated that prior knowledge has a significant role on eye metrics such as fixation. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 11. Significance of Study  Understanding students’ interpretation of microscope slides in relation to their level of content knowledge and their expertise level, which is developed through repetition, holds a merit for the future use of such visual materials in education.  Moreover, understanding the effect of prior knowledge and prior exposure with or without a well-designed environment is also important in terms of science education. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 12. Purpose of the Study  Shedding light on how students conduct visual search and interpret microscope slides in relation to their level of pre-existing content knowledge can help in the use and development of visual aids to support learning process in education.  Therefore, the purpose of this study is to target novice individuals who are lack of repetition and expertise in interpreting microscope slides and to investigate their visual search processes. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 13. Research Questions Do the eye movements during the visual search task in different background colour contrast differ in terms of individual’s prior knowledge level? (high / low color contrast)?  Do the eye movements during the visual search task in high colour contrast images differ in terms of individual’s prior knowledge level?  Do the eye movements during the visual search task in low colour contrast images differ in terms of individual’s prior knowledge level?  Do the eye movements during the visual search task in different background colour contrast differ in terms of having prior exposure to microscope view? Do the eye movements during the visual search task in high colour contrast images differ in terms of having prior exposure to microscope view? Do the eye movements during the visual search task in low colour contrast not images differ in terms of having prior exposure to microscope view?  Is the interaction effect between prior knowledge and prior exposure significant? Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 15. Study Group  Prior content knowledge test was administered a total of ninety-eight, 3rd and 4th year undergraduate students .  All participants were pre-service elementary science teachers who completed at least two general biology courses and had the basic skills of microscope use.  Participants were grouped based on their prior content knowledge levels on the phases of mitosis as high or low level groups.  40 students were appointed to either high or low level groups, 20 students to each group.  For the purpose of this study, all participants were novices in terms of their experience of using microscopes. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 16. Demographic characteristics Demographic Characteristics F Percentage (%) Female 28 70% Gender Male 12 30% 3 28 70% Grade 4 12 30% Low 20 50% Prior Knowledge High 20 50% Yes 20 50% Prior exposure to microscope view No 20 50% Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 17. Data Collection Tools The prior knowledge Test: This content test consisted of 10 multiple choice questions which was developed by a field expert who is also one of the researchers. The item discrimination indices for the questions ranged between 0.32 and 0.66. KR- 20 reliability coefficient was found to be .717. Each correct answer was scored 1 point and wrong answer was scored 0 point. Visual Search Questions: Seven different microscope images of cells were displayed on a computer for each question. Question images were grouped in two sets; high and low contrast. The first set included four questions which were high contrast images with maroon coloured genetic material on a light blue cell . Second set included three low contrast slides with orange colored genetic material on a yellow cell background. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 18. Experimental Setting  All of the cell images were transformed to an experiment set by Tobii software on eye tracker.  Tobii T120 Eye tracker was used to record eye movement data by two binocular infra-red cameras integrated within the panels of the monitor.  Fixation duration and time to first fixation were obtained from eye tracker for this study.  Undergoing mitosis phases on the cells were drawn as area of interests. Those area of interests were the areas which students required to find and recognize the right mitosis phases.  Participants’ eye metrics data were recorded during the process and eye movement data on those areas were collected to analyse. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 19. Area of interest samples from different background colour contrasts questions  Participants were asked to find the cell that is undergoing through any mitotic phase and identify the cell by clicking on it with the curser and then to select the name of the mitotic phase among multiple choices presented on the right side of the screen.  Each of the questions was displayed 20 seconds on the screen. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 20. Data Analysis  A 2x2 within subject design is used for the purpose of this study.  Participants were grouped by their level of prior content knowledge (high and low) and their statement on either having a prior exposure to slides showing phases of mitotic division with the consideration of priming effect.  Dependent variables: Time to first fixation, fixation duration Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 22. Eye movement statistics across prior knowledge and prior exposure groups Low level prior knowledge High level prior knowledge Prior exposure No Prior exposure Prior exposure No Prior (n=10) (n=10) (n=10) exposure (n=10) M 1.46 1.29 2.79 2.81 Fix.Dur. Sd .806 .764 1.94 Blue 1.27 M 7.04 7.52 4.66 3.98 T_FirstFix. Sd 5.2 5.07 3.61 4.33 M .818 .946 1.28 .889 Fix.Dur. Yellow Sd .728 .813 .852 .697 M 4.93 3.52 3.84 5.18 T_FirstFix Sd 3.24 2.87 2.31 5.22 Descriptive analyses indicate that for high contrast images, students with higher content knowledge (a) fixate on the cells faster in comparison to students with lower content knowledge and (b) have a longer fixation period of time on the images. For further analysis, a two-factor MANOVA was conducted. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 23. MANOVA Results  The main effect of prior knowledge was found significant (Wilk’s Λ = . 677), F(4, 33) = 3.932, p =.010, ηp2 = .323).  Neither the main effect of prior exposure to microscope view (Wilk’s Λ =. 989), F(4, 33) = 0.91, p = .985, ηp2 = .011) nor the interaction effect of prior knowledge and prior exposure to microscope view (Wilk’s Λ = . 880), F(4, 33) = 1.129, p =.369, ηp2 = .120) were found significant.  High level prior knowledge group was fixated on the area of interests (time to first fixation) significantly in a shorter time than low level prior knowledge group in blue background questions (high contrast); F(1, 36)=4.14, p=.049, ηp2 = .103. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 24. MANOVA Results  In the blue background (high contrast) questions, total fixation duration of high level prior knowledge group was found to be higher than their low level counterparts F(1, 36)=12,183, p=.001, ηp2 = .253.  However, in yellow background (low contrast) questions, no statistically significant difference between prior knowledge level groups was found neither in time to first fixation nor in total fixation duration in the area of interests. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 25. DISCUSSION  This study investigated the effect of prior knowledge and prior exposure in different colour contrasted microscope images.  The images used as questions in the study were designed in two different colour contrasts, high contrast; maroon targets on blue background versus low contrast; orange targets on yellow background, to examine whether the colour contrast facilitate the recognition process in visual search tasks.  The findings revealed out that for high colour contrast image group prior knowledge has an effect in visual search tasks both in time to first fixation, which shows recognition, and fixation duration on the related areas. This finding is consistent with individual differences principle of Mayer (2001) in the multimedia design, which states that prior knowledge has a compensating effect on producing own mental models in multimedia learning (Mayer &Moreno 1998). Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 26. DISCUSSION • In high colour contrast questions, high level prior knowledge group recognized area of interest in a shorter time and fixated on correct areas longer than other areas. This finding is also consistent with prior research which reports that combinations of colours that had higher levels of contrast between background and foreground and also higher prior knowledge generally led to better performance than combinations of lower (Ling and Schaik 2002; Theeuwes, Reimann and Mortier 2006; Huang 2008; Zhuang and Papathomas 2011). Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 27. DISCUSSION  On the other hand, visual performance of high level prior knowledge group and low level prior knowledge group was found to be similar on low contrast images.  Nevertheless, based on the findings of this study it is difficult to suggest that prior knowledge effect has disappeared in low contrast background microscope images.  It should be noted that time to first fixation occurs in a shorter period of time, and the difference is greater for low level prior knowledge group with no prior exposure between high and low contrast pictures.  Follow up to explore this difference, data were revisited and the length of first fixation duration metric results were also computed in order to explore whether these first fixations would be taken as an evidence of visual search or a mere scanning process. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 28. DISCUSSION  The length of first fixation durations were shorter in low contrast pictures.  The low contrast pictures had a quicker fixation but less time spent whereas those studying the high contrast pictures had relatively fixated later but stayed longer on the areas of interests.  It can be speculated that their first fixation could just be interpreted as an indication of mere eye scanning on the picture. Since visual search and scanning are different cognitive processes, additional data is required to be able to predict if the prior knowledge affect disappeared or not.  Furthermore, fixation duration is stated to being an indicator of the cognitive complexity (Henderson and Hollingworth 1998). Based on the findings in this study, it can be speculated that low contrast images might increase the cognitive complexity for participants. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 29. CONCLUSION  In conclusion, the role of visual representations cannot be ignored in science education.  This study investigated visual search process by the measurement of eye movements which is an objective measurement of cognitive processes. The results revealed that for novices, who are lack of expertise in interpreting microscope images, such as participants of this study, prior knowledge has an effect when high contrast images were in question.  Findings of this study can be useful for both teachers and instructional designers in science education. Instructional designers should consider design principles together with individual differences when developing visual displays.  Teachers also should consider individuals’ abilities and properties of visual materials when choosing instructional materials. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012
  • 30. CONCLUSION  Individual differences (i.e. visual ability, prior knowledge, visual attention, working memory, visual perception) are important in understanding the visual materials and also in learning from multimedia environments.  However, effect of individual differences could be sensitive to the design of the learning environment.  Although people could benefit from their positive individual characteristics in well-designed learning environments, ever so novel or familiar, increase in the complexity of material would diminish the advantage of previous knowledge.  Reversely, well designed display designs found to be facilitating comprehension of students who has low prior knowledge  Further research could explore the effects of other individual differences such as gender, age, and distinctive socio-cognitive characteristics on visual processes in multimedia environments. Sönmez, D, Altun, A.& Mazman, S.G. Hacettepe University/ TURKIYE CELDA 2012