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1. ISOLATING BACTERIA FROM TROPICAL
CONTAMINATED SOILS IN PUERTO RICO
Sara Diaz
Patricia Rodriguez
Camille Ruiz
Justin Cotto

2. SAMPLING LOCATIONS
Sample Coordinates Environmental description Location Date/Time
Ruiz C
- #1
18°6’55.1”N;
66°9’36.2”W
Moist (underneath grass), 5 cm;
72°F
Cayey, PR January/28/15
7:30 p.m.
Cotto J
- #2
18.15º N
66.18º W
Moist environment (above
grass), 76ºF
Cidra, PR January/29/15
8:00 a.m.

3. SAMPLING LOCATIONS
Sample Coordinates Environmental description Location Date/Time
Diaz S
-#1
18°15'15.7"N
66°06'06.9"W
Moist environment
80°F
Aguas
Buenas, P.R.
4:26 p.m.
Rodríguez P
-#2
18°15'15.7"N
66°06'06.9"W
Moist environment
80°F
Aguas
Buenas, P.R.
4:26 p.m.

4. Process CLRV30TSA01A CLRV30M01B JCR30TSA01A
Grand Stain Negative Positive Positive
Bacterial Morphology Coccus Bacillus Bacillus
PCR/Electrophoresis #1 Negative Negative Negative
PCR/Electrophoresis #2 Positive Negative -
Antibiotic Production Negative Negative Negative

5. Process S15UPRC-RISESCDM30T01B S15UPRC-RISEPR30M01
Gram Stain Positive Positive
Bacterial Morphology Bacillus Bacillus
PCR/Electrophoresis #1 Negative Negative
PCR/Electrophoresis #2 Negative Negative
Antibiotic Production Negative Negative

6. ANTIBIOTIC RESISTANCE TEST
RESULTS
Antibiotic CLRV30TSA01A CLRV30M01B JCR30TSA01A
Kanamycin 2.0 cm 1.0 cm 2.0 cm
Penicillin 0.0 cm 0.0 cm -
Tetracycline 1.6 cm 2.4 cm 2.2 cm
Gentamicin 1.9 cm 0.9 cm -
Chloramphenicol 0.0 cm 0.0 cm 2.5 cm
Bacitracin 0.0 cm 0.0 cm 1.5 cm
Vancomycin 0.0 cm 0.0 cm -
Streptomycin 1.6 cm 1.0 cm 1.9 cm

7. ANTIBIOTIC RESISTANCE TEST
RESULTS
Antibiotic S15UPRC-RISESCDM30T01B
Streptomycin 2.2 cm
Chloramphenicol 2.3 cm
Erythromycin 1.0 cm
Kanamycin 1.6 cm
Gentamicin 0.6 cm
Antibiotic S15UPRC-RISEPR30M01
Rifampin 0.13 cm
Kanamycin 0.16 cm
Vancomycin 0.17cm
Streptomycin 0.30 cm
Tetracyclin 0.14 cm