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Environmental Microbiology

Biotechnology is the integration of natural sciences and engineering in order to achieve the

application of organism, cells, part thereof and molecular analogues for product and

services (EFB, 1989). Environmental biotechnology is the application of these processes

for the protection and restoration of the quality of our environment.

 Bioremediation is the use of biological systems for the reduction of pollution from air or

from aquatic or terrestrial systems. Microorganism and plants are the biological systems

which are generally used. Biodegradation processes may or may not need air. A complete

biodegradation results in detoxification by mineralizing pollutants to carbon dioxide, water

and harmless inorganic salts. Incomplete biodegradation        will yield breakdown products

which may or may not be less toxic than the original pollutant.

  Bioremediation techniques can be used to reduce or remove hazardous waste which has

already polluted the environment. Some applications of bioremediation are


Waste water and industrial effluents:

 Microorganisms in sewage treatment plants remove the more common pollutants from

waste water before it is discharged into rivers or the sea. Heavy metals sulphur compounds

can be removed from waste by the aid of sulphur metabolizing bacteria.


Drinking and process water

   Not only water need to be recycled in the development of sustainable use of resources,

overall quality must also be improved to satisfy the consumers. In many agricultural

regions of the world , animal wastes and excess fertilizers result in high levels of nitrates in




                                                                                              1
drinking water. Biotechnology has provided successful methods by which these

compounds can be removed from processed water before it is delivered to customers.


Soil and land treatment

Both in situ and ex situ methods are commercially exploited for the cleanup of soil and the

associated groundwater. In situ treatments may include the introduction of micro

organisms, ventilation and/ or adding nutrient solutions. Ex situ treatment involves

removing the soil and groundwater and treating it above ground. Bioremediation

(biorestoration) of land is often cheaper than physical methods and its product are harmless

if complete mineralization takes place.           Bioremediation using plant is called

phytoremediation. This technique is already used to remove metals from contaminated soils

and groundwater and is being further explored for the remediation of other pollutants. The

combined use of Plants and bacteria live closely associated with the roots of plants and

depend on substances excreted by the roots. Such rhizo-bacteria whose numbers are much

higher than those of other soil bacteria, may be genetically modified to break down other

pollutants.


Solid waste

Anaerobic digestion of solid wastes in high rate anaerobic digesters has gained increasing

public acceptance because it permits the recovery of substantial amounts of high value

biogas together with a high quality stable organic residue and this without giving rise to

environmental nuisance.

Anaerobic digestion of mixed solid waste may be an important step in recycling of solid

wastes and constitute an alternative to incarnation.




                                                                                          2
Prevention

More industrial companies are developing processes with reduced environmental impact

responding to the international call for the development of a sustainable society.


Process Improvement

 Many industrial processes have been made more environmentally friendly by the use of

enzymes. Enzymes are biological catalysts that are highly efficient and have numerous

advantages over non biological catalysts. They are non toxic and biodegradable, work best

in moderate temperatures and in mild conditions and have fewer side reactions than

traditional method because they are highly specific. Production methods that employ

enzymes are generally not only cleaner and safer compared with other methods, but mostly

also more economic in energy and resource consumption.


Product innovation

Biotechnology also can help to produce new products which have less impact on the

environment than their predecessors. The use genetically modified plant varieties that are

resistant against insects and/or diseases may considerably minimize the use of pesticides

which not only prevents the use of the mostly non- renewable raw materials, energy and

labor necessary for their production but also will reduce the negative impact of their

residues.




                                                                                        3
Detection and Monitoring

Detection and monitoring of pollutants

Biological methods are already in use to detect pollution and for the continuous monitoring

of pollutants. Established measures include: counting the number of plants, animals and

microbial species. More recently, biological detection methods biosensors and

immunoassays have been developed and are now being commercialized.


Detection and monitoring of microorganisms used for bioremediation

When laboratory grown micro-organisms are inoculated into a bioremediation site

(bioaugmentation) is often becomes necessary to monitor their presence and/or

multiplication to check the progress of the process.


Detection and monitoring of ecological effects

Bioremediation is aimed at improving the quality of the environment by removing

pollutants.


Conclusion

There is a potential for biotechnology to make a further major contribution to protection

and remediation of the environment. Hence biotechnology is well positioned to contribute

to the development of a more sustainable society.




                                                                                         4

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Environmental Microbiology

  • 1. Environmental Microbiology Biotechnology is the integration of natural sciences and engineering in order to achieve the application of organism, cells, part thereof and molecular analogues for product and services (EFB, 1989). Environmental biotechnology is the application of these processes for the protection and restoration of the quality of our environment. Bioremediation is the use of biological systems for the reduction of pollution from air or from aquatic or terrestrial systems. Microorganism and plants are the biological systems which are generally used. Biodegradation processes may or may not need air. A complete biodegradation results in detoxification by mineralizing pollutants to carbon dioxide, water and harmless inorganic salts. Incomplete biodegradation will yield breakdown products which may or may not be less toxic than the original pollutant. Bioremediation techniques can be used to reduce or remove hazardous waste which has already polluted the environment. Some applications of bioremediation are Waste water and industrial effluents: Microorganisms in sewage treatment plants remove the more common pollutants from waste water before it is discharged into rivers or the sea. Heavy metals sulphur compounds can be removed from waste by the aid of sulphur metabolizing bacteria. Drinking and process water Not only water need to be recycled in the development of sustainable use of resources, overall quality must also be improved to satisfy the consumers. In many agricultural regions of the world , animal wastes and excess fertilizers result in high levels of nitrates in 1
  • 2. drinking water. Biotechnology has provided successful methods by which these compounds can be removed from processed water before it is delivered to customers. Soil and land treatment Both in situ and ex situ methods are commercially exploited for the cleanup of soil and the associated groundwater. In situ treatments may include the introduction of micro organisms, ventilation and/ or adding nutrient solutions. Ex situ treatment involves removing the soil and groundwater and treating it above ground. Bioremediation (biorestoration) of land is often cheaper than physical methods and its product are harmless if complete mineralization takes place. Bioremediation using plant is called phytoremediation. This technique is already used to remove metals from contaminated soils and groundwater and is being further explored for the remediation of other pollutants. The combined use of Plants and bacteria live closely associated with the roots of plants and depend on substances excreted by the roots. Such rhizo-bacteria whose numbers are much higher than those of other soil bacteria, may be genetically modified to break down other pollutants. Solid waste Anaerobic digestion of solid wastes in high rate anaerobic digesters has gained increasing public acceptance because it permits the recovery of substantial amounts of high value biogas together with a high quality stable organic residue and this without giving rise to environmental nuisance. Anaerobic digestion of mixed solid waste may be an important step in recycling of solid wastes and constitute an alternative to incarnation. 2
  • 3. Prevention More industrial companies are developing processes with reduced environmental impact responding to the international call for the development of a sustainable society. Process Improvement Many industrial processes have been made more environmentally friendly by the use of enzymes. Enzymes are biological catalysts that are highly efficient and have numerous advantages over non biological catalysts. They are non toxic and biodegradable, work best in moderate temperatures and in mild conditions and have fewer side reactions than traditional method because they are highly specific. Production methods that employ enzymes are generally not only cleaner and safer compared with other methods, but mostly also more economic in energy and resource consumption. Product innovation Biotechnology also can help to produce new products which have less impact on the environment than their predecessors. The use genetically modified plant varieties that are resistant against insects and/or diseases may considerably minimize the use of pesticides which not only prevents the use of the mostly non- renewable raw materials, energy and labor necessary for their production but also will reduce the negative impact of their residues. 3
  • 4. Detection and Monitoring Detection and monitoring of pollutants Biological methods are already in use to detect pollution and for the continuous monitoring of pollutants. Established measures include: counting the number of plants, animals and microbial species. More recently, biological detection methods biosensors and immunoassays have been developed and are now being commercialized. Detection and monitoring of microorganisms used for bioremediation When laboratory grown micro-organisms are inoculated into a bioremediation site (bioaugmentation) is often becomes necessary to monitor their presence and/or multiplication to check the progress of the process. Detection and monitoring of ecological effects Bioremediation is aimed at improving the quality of the environment by removing pollutants. Conclusion There is a potential for biotechnology to make a further major contribution to protection and remediation of the environment. Hence biotechnology is well positioned to contribute to the development of a more sustainable society. 4