Seminario Biología Molecular, Tercer semestre de Medicina. Universidad Pontificia Bolivariana

1. THESIS
DEFENSE
Presented By : Adeline Palmerston
Katherine Gomez Serna
UPB| III semestre

2. ENDORREPELIN
2
-Fragment of perlecan, heparan
sulfate proteoglycan
-Anti-angiogenesis properties
Angiostasis
Inhibiting blood
vessel formation

3. ANGIOGENESIS
3
Process that forms new
blood vessels, crucial in
biological functions
Consists of the migration,
growth and differentiation
of endothelial cells

4. Evaluate how endorepellin
downregulation promotes
angiogenesis after experimental
traumatic brain injury
Objective
4

5. Used as primary antibodies: CD31
and Ki-67. Helped to quantify
these markers of growth to
identify angiogenesis
Revealed increased expression of
VEGF and angiopoietin-1 in mice
treated with AAV-Ep-green
fluorescent protein
5
METHODS
Inmunostaining Western blotting

6. Endorepellin downregulation
promoted angiogenesis and
increased cortical
neovascularization
To identify the gen GAPDH
expression which plays a key role in
cellular energy production,
regulating endothelial cell
migration and tube formation
6
METHODS
Synchrotron radiation
angiography
Real-time PCR

7. RESULTS
7

8. RESULTS
7

9. DISCUSSION
8
Author
What did the author
said?
Agree or disagree
Abrahamson EE, Ikonomovic MD
Brain injury-induced dysfunction of the
blood
brain barrier as a risk for dementia.
Agree
Goyal A, Pal N, Concannon M, Paul M,
Doran M, Poluzzi C, Sekiguchi K,
Whitelock JM,
Neill T, Iozzo RV
Endorepellin, the angiostatic module of
perlecan, interacts with both the
alpha2beta1 integrin and vascular
endothelial growth factor receptor 2
(VEGFR2): a dual receptor antagonism.
Agree
Kapoor A, Chen CG, Iozzo RV
Endorepellin evokes an angiostatic
stress signaling cascade in endothelial
cells.
Agree

10. CONCLUSIONS
First
Understanding the effects of
molecular biology within its
methods show us that it can
have an important influence
in a lot of aspects in
medicine, not only in
neurological disorders also
in cancer and multiple
affections that are
presented in our society
9
The study highlights the
potential of AAV-mediated
gene therapy targeting
Endorepellin to modulate
angiogenesis in traumatic
brain injury, showcasing the
importance of molecular
biology in developing novel
therapeutic strategies for
neurological disorders
Second

11. Larana University | 2024
10

12. THANK YOU
Presented by: Katherine Gomez