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The Indispensable Forensic Science Tool DNA:
Why is DNA an Indispensable Tool Forensic Science? ,[object Object]
[object Object],[object Object],[object Object]
Its Uses ,[object Object]
The Angel of Death: Josef Menegle ,[object Object],[object Object],[object Object],[object Object]
Paternity Cases ,[object Object],1. 2. 1. 2.
Homicide or Rapes: OJ Simpson
Exoneration ,[object Object],[object Object],[object Object],[object Object]
Exoneration
What is DNA? ,[object Object],[object Object],[object Object]
James Watson & Francis Crick ,[object Object],[object Object],[object Object],[object Object]
What is DNA? ,[object Object],[object Object]
[object Object]
A T A G C C A A C C A A T A T C G G T T G G T T Replication of a single strand of DNA
DNA at Work ,[object Object],[object Object],[object Object]
How can the DNA sequence dictate the sequence for a particular protein? ,[object Object],[object Object],[object Object]
U A U C G G U U G G U U A T A G C C A A C C A A So, a DNA Sequence of:   Would have an mRNA sequence of : Use the following table to determine which of the 20 amino acids would make up this sequence.  Each group of three nucleotide bases, an RNA triplet codon, is read in order to determine which amino acid is placed in the sequence.  The sequence determines the type of protein that is produced. U A U   C G G   U U G  G U U DNA template Strand RNA Strand of Transcribed DNA
U A U C G G U U G G U U
U A U   C G G   U U G  G U U mRNA Sequence: Would result in this protein amino acid sequence Tyrosine Arginine Leucine Valine Tyrosine Arginine Leucine Valine
U A U   C G G   U U  C  G U U a one base change in the mRNA Sequence: Would result in this protein amino acid sequence U A U   C G G   U U G  G U U Tyrosine Arginine Phenylalaine Valine Tyrosine Arginine Phenylalaine Valine
II.  DNA Profiling ,[object Object],[object Object],[object Object],[object Object]
Discovery – Sir Alec Jeffreys ,[object Object],[object Object]
DNA Analysis   A New Technique –- Why Use It? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
How Does It Work? ,[object Object],[object Object],[object Object],[object Object]
Potential Sources of DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The 2 Main Types of DNA Profiling ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
1.  RFLPs ,[object Object],[object Object],[object Object],[object Object]
RFLP: Electrophoresis ,[object Object],[object Object],[object Object]
RFLP: Autoradiograph
[object Object]
How unique are these profiles? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Paternity  ,[object Object],[object Object]
Interpret the results of the following DNA fingerprints ,[object Object]
[object Object]
2.  STRs ,[object Object],[object Object],[object Object]
 
 
 
 
 
How do we get so much variation? Recall inheritance patterns...
How do we get so much variation? Recall inheritance patterns... ,[object Object],[object Object],[object Object],[object Object],[object Object]
Automated STR Test
Crime Scene Samples &  Reference Samples ,[object Object],[object Object]
Extract and Purify DNA ,[object Object]
PCR Amplification ,[object Object],[object Object]
 
Genetic Analyzer: SIZE, COLOR  & AMOUNT
Genetic Analyzer:  Capillary Electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Detector Window

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Forensic Dna Me

  • 1. The Indispensable Forensic Science Tool DNA:
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7. Homicide or Rapes: OJ Simpson
  • 8.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14. A T A G C C A A C C A A T A T C G G T T G G T T Replication of a single strand of DNA
  • 15.
  • 16.
  • 17. U A U C G G U U G G U U A T A G C C A A C C A A So, a DNA Sequence of: Would have an mRNA sequence of : Use the following table to determine which of the 20 amino acids would make up this sequence. Each group of three nucleotide bases, an RNA triplet codon, is read in order to determine which amino acid is placed in the sequence. The sequence determines the type of protein that is produced. U A U C G G U U G G U U DNA template Strand RNA Strand of Transcribed DNA
  • 18. U A U C G G U U G G U U
  • 19. U A U C G G U U G G U U mRNA Sequence: Would result in this protein amino acid sequence Tyrosine Arginine Leucine Valine Tyrosine Arginine Leucine Valine
  • 20. U A U C G G U U C G U U a one base change in the mRNA Sequence: Would result in this protein amino acid sequence U A U C G G U U G G U U Tyrosine Arginine Phenylalaine Valine Tyrosine Arginine Phenylalaine Valine
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 26.
  • 27.  
  • 28.
  • 29.
  • 31.
  • 32.
  • 33.
  • 34.
  • 35.
  • 36.
  • 37.  
  • 38.  
  • 39.  
  • 40.  
  • 41.  
  • 42. How do we get so much variation? Recall inheritance patterns...
  • 43.
  • 45.
  • 46.
  • 47.
  • 48.  
  • 49. Genetic Analyzer: SIZE, COLOR & AMOUNT
  • 50.