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Stian Soiland-Reyes
myGrid, School of Computer Science
University of Manchester, UK
UKOLN DevSci: Workflow Tools
Bath, 2010-11-30Gridmy
http://taverna.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
What is myGrid?
 An e-Science Collaboration Since 2001
 Not a grid!
 Numerous partners involved:
 University of Manchester
 University of Southampton
 University of Oxford
 EMBL-EBI
 Provides sustainable and production quality software
 Supported by OMII-UK, EPSRC and BBSRC
 Mixture of developers, bioinformaticians and
researchers
Software | Services | Content | Skills | Community
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Motivation
 Challenge:
Bioinformatics
 Large amounts of data
 Many open questions
 Numerous freely
available public
datasets and analysis
tools
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Huge amounts of data
100+ Genes
QTL regions
Microarray
1000+ Genes
Next Gen
Sequencing
10,000+
Genes
How do I look
at all the genes
systematically?
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Manual approach
 Search using public web sites and databases
 Pubmed
 Uniprot
 EBI BioMart
 Copy and paste to web tools for analysis
 NCBI Blast
 EBI InterPro
 Further processing locally
 R
 Perl
 Python
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Manual: disadvantages
• Scale of analysis task overwhelms researchers
– lots of data
• User bias and premature filtering of datasets –
cherry picking
• Hypothesis-Driven approach to data analysis
• Constant changes in data - problems with re-
analysis of data
• Implicit methodologies (hyper-linking through
web pages)
• Error proliferation from any of the listed issues
– notably human error
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Web services and workflows
 Web services
 Technology and standards for exposing code and
data resources that can be programmatically
consumed by a remote third party
 Description on how to interact with the service,
parameters, documentation
 Workflows
 General technique for describing and executing a
process
 Describe what you want to do running which
services
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Taverna workflows
 A set of (local and remote)
services to analyze or manage
data
 Nested workflows are also
services
 Data-links connects services
 i.e. output from service A is input to
service B and C
 Describes the desired dataflow
instead of process coordination
 Automatic iterations
 Can customize list handling and
control links
Get_pathways
Workflow Inputs
Workflow Outputs
Workflow Inputs
Workflow Outputs
remove_uniprot_duplicates
merge_uniprot_ids
species
getcurrentdatabase
kegg_pathway_release
binfo
regex_2
split_for_duplicates
split_for_duplicate_pathways
remove_duplicate_kegg_genes
merge_genes_and_pathways_3
flatten_pathway_files
merged_pathways
merge_genes_and_pathways
merge_genes_and_pathways_2
merge_kegg_references
kegg_external_gene_reference
remove_pathway_duplicates
merge_pathway_desc
merge_pathway_list_1
merge_pathway_list_2
remove_duplicate_ids
merge_patwhay_ids
pathway_descriptions
merge_reports
report
merge_gene_desc
remove_nulls_3
gene_descriptions
gene_ids
REMOVE_NULLS_2
remove_entrez_duplicates
merge_entrez_genes
remove_pathway_nulls
remove_Nulls
concat_kegg_genes
split_gene_ids
remove_pathway_nulls_2
add_uniprot_to_string
gene_descriptions pathway_descriptions
add_ncbi_to_string
Kegg_gene_ids_2
pathway_ids
Kegg_gene_ids
genes_in_qtl
mmusculus_gene_ensembl
create_report
ensembl_database_releasegenes_pathways kegg_pathway_release
Merge_pathways
concat_ids
pathway_ids
regex
split_by_regex
lister
Merge_gene_pathways
pathway_genes
concat_gene_pathway_ids
get_pathways_by_genes1
chromosome_namestart_position end_position
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
What types of services?
 Public/private/secured WSDL/SOAP web services
 RESTful web services
 Spreadsheet import
 Command line tools (local/ssh)
 Inline scripts (Beanshell, R)
 Java APIs
 Customizations:
 BioMart, BioMoby / SADI
 Soaplab
 Grid services (Globus, EGEE gLite, caGrid)
 … your tool (Plugin tutorial on wiki)
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Which services?
 Taverna is general, can connect to standard
web services for any domain
 Bioinformatics:
 From professional third-party organisations
providing robust & open data/analysis services
 ..to under-the-desk web services for one particular
purpose, ran by PhD students
  http://biocatalogue.org/ - 1730 services from 130
providers – crowd sourced and quality monitored
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Taverna
workbench
 Graphical desktop tool
 No server installation
required
 Drag-and-drop services
into diagram
 Connect services, run,
reconnect, rerun
 Integrates diverse set
of tools
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Sharing workflows
 myExperiment.org allows users to share,
find, download and rate workflows
 “Facebook for the scientist”
 3000 members, 1100 workflows
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Extensible UI and engine
 Plugins can provide new “perspectives”
 i.e.: BioCatalogue, myExperiment
 Provide service-specific customization
 BioMart interface replicates web site
 Adding new functionality
 Looping, branching, dynamic service resolution
 New service types
 Design helpers, “Find matching service”
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Taverna 3 “Next-gen”
 Under development for 2011
 Interactive, component-centric and data-centric
workflow design
 Pre-packaged workflow components
 Searching for workflow components from
BioCatalogue and myExperiment
 New myGrid workflow components library
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Taverna command line
 Executes from a
Windows/Linux/OSX
shells
 Takes a predefined
workflow with files as
inputs and outputs
 Quick way to
“productionize” a
workflow
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Taverna Server
 REST/SOAP interface to
execute workflows
 Client libraries for Ruby and Java
 Two demonstration web interfaces
 Ruby
 Java Portlets
 Future
 Detailed execution support and control
 Security delegation
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Taverna portlet
 Example portlet
implementation
 Executes workflows
using Taverna Server
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Ruby web interface
 Example customized
web interface
 Uses Ruby gem
t2-server
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Taverna on the cloud
 Use-case:
 SNP analysis and annotation of
genome sequenced from
breeds of cows in Africa – why are
some of them resistent to X?
 Amazon EC2 with Taverna Server and local
services
 Custom (built-in-a-week) Ruby on Rails web
interface
 Runs through 31 chromosomes in 6.5 hours
using 10 instances - $26
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Open source, open development
 Taverna suite of tools are all open source
and free to use
 Large user community, active mailing lists
 Lead developers: myGrid in Manchester
 Contributors from across the world
 PAL programme
 myGrid provides training, tutorials and
documentation
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Acknowledgements
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
Gridmy
http://taverna.org.uk/http://mygrid.org.uk/
More information
 http://www.mygrid.org.uk/
 http://www.taverna.org.uk/
 http://www.myexperiment.org/
 http://www.biocatalogue.org/

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