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Unilag workshop complex genome analysis
1.
COMPLEX GENOME ANALYSIS
Ikhide Imumorin, PhD Assistant Professor of Animal Molecular & Quantitative Genetics Cornell University Ithaca, NY 14853 USA
2.
GENOMICS ANALYSIS
3.
The genomes of
living organisms vary enormously in size.
4.
5.
6.
Fig. 10.2
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
Physical mapping by
analysis of STSs Bottom-up approach Each STS represents a unique segment of the genome amplified by PCR. Fig. 10.5
18.
19.
Chromosome 7 at
three levels of resolution Fig. 10. 6 c
20.
FISH protocol for
top-down approach Fig. 10.8
21.
22.
23.
24.
25.
26.
27.
28.
29.
Conserved segments of
syntenic blocks in human and mouse genomes Fig. 10.12
30.
31.
32.
Protein coding genes
generate the proteome How transcription factor protein domains have expanded in specific lineages Fig. 10.11
33.
Examples of domain
accretions in chromatin proteins Fig. 10.13
34.
Number of distinct
domain architectures in four eukaryotic genomes Fig. 10.14
35.
36.
Repeat sequences constitute
more than 50% of the genome. Fig. 10.15
37.
38.
Genome has a
distinct organization. Gene family – olfactory receptor gene family
39.
Class II region
of human major histocompatibility complex contains 60 genes in 700 kb Fig. 10.17
40.
41.
42.
43.
44.
45.
46.
47.
High-throughput instruments DNA
sequencer Fig. 10.20
48.
High-throughput instruments e.g,
microarrays Fig. 10.21
49.
Two color -
DNA microarray Fig. 10.22
50.
51.
Lynx therapeutics sequencing
strategy of MPSS Fig. 10.24
52.
53.
54.
Notas del editor
Figure 10.2
Figure 10.3
Figure 10.4
Figure 10.5
Figure 10.5 a, b
Figure 10.5 c
Figure 10.8
Figure 10.9
Figure 10.10
Figure 10.12
Figure 10.13
Figure 10.16
Figure 10.14
Fig. 10.15
Figure 10.20
Figure 10.18 (top) / Figure 10.19a (bottom)
Figure 10.20
Figure 10.21
Figure 10.22
Figure 10.24
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