National Agricultural Innovation Project (NAIP), ICAR and the International Food Policy Research Institute (IFPRI) organized a two day workshop on ‘Impact of capacity building programs under NAIP’ on June 6-7, 2014 at AP Shinde Auditorium, NASC Complex, Pusa, New Delhi. The main purpose of the workshop was to present and discuss the findings of the impact evaluation study on capacity building programs under NAIP by IFPRI. The scientists from ICAR and agricultural universities were sent abroad to receive training in specialized research techniques. Post-training, scientists were expected to work on collaborative projects within the ICAR, which would further enrich their knowledge and skills, expand their research network and stimulate them’ to improve their productivity, creativity and quality of their research. The ICAR commissioned with IFPRI (International Food Policy Research Institute) to undertake an evaluation of these capacity building programs under NAIP in July 2012. The workshop shared the findings on the impact of capacity building programs under NAIP and evolve strategies for future capacity building programs

1. K.C. Bansal
National Bureau of Plant Genetic Resources
Pusa Campus, New Delhi – 110 012
www.nbpgr.ernet.in
National Genomic Resources
Repository

2. PGR Utilization and
Preparedness for
future
PGR Collection,
Conservation,
Exchange and
Quarantine
PGR
Informatics &
IP Protection
Genomics and
National
Genomic
Resources
Repository
PGR
Characterization
and Evaluation
(phenotypic and
genotypic)
NBPGR major activities

3. Developmentandconservationof
genomicresources Collection, validation and conservation of genomic
resources
Basic and supportive research in genomics
and bioinformatics
Gene discovery and allele mining for specific traits
(biotic & abiotic stresses, quality) from plant
genetic resources
Development and validation of new molecular marker
systems in priority crops and molecular diagnostics for
transgenes
National Genomic Resources Repository

4. Plant Genetic Resources / FirstView Article pp 1-10
Copyright © NIAB 2014
DOI: Published online: 20 February 2014
Research Article
Next-generation genebanking: plant genetic resources
management and utilization in the sequencing era
Rob van Treurena1 c1 and Theo J. L. van Hintuma1
a1 Centre for Genetic Resources, the Netherlands (CGN), Wageningen University
and Research Centre, PO Box 16, NL-6700 AA Wageningen, The Netherlands
DNA sequencing as an integral component for generation
and conservation of plant genetic resources

5. Why conserve genomic resources?
• Current research generates a lot of genomic resources
▫ Routine cloning experiments
▫ Genome sequencing projects
• The genome resources are indispensable tools for post-
genomic research
▫ Physiological and morphological characterization of a
species, functional analysis of genes, comparative genomics
and plant and animal breeding
• It is necessary to maintain an efficient system for
conservation and management of genomic resources

6. Salient Points
I. Types of genomic resources
II. Modalities of operation
III. Documentation and Database
IV. Infrastructure, space and human resources
V. Storage methodologies
VI. Research component
VII. Policy issues

7. I. Types of genomic resources
• Cloning vectors, expression vectors, binary
vectors, RFLP probes
• Cloned genes, promoters fused to reporter genes
• Sub-genomic, cDNA , EST, repeat enriched libraries
• BAC, YAC, PAC clone set from sequencing projects
• Genomic, mitochondrial or chloroplast DNA
• Cloned DNA from wild and weedy species
produced exclusively for the repository

8. Plant DNA banks world-wide
Genomic DNA ONLY
Plant DNA Bank in Korea (PDBK)
Graduate School of Biotechnology, Korea University, Seoul, Korea.
Royal Botanic Gardens Kew DNA Bank
22,000 samples of plant genomic DNA, all stored at -80°C.
The Australian Plant DNA Bank
Australian native and other important crop plant species; also transgenic organisms developed through research
DNA Banking at the Missouri Botanical Garden
To promote phylogenetic research while easing the demand on herbarium specimens
DNA Bank Brazilian Flora Species
Rio de Janeiro Botanical Garden, Brazil
DNA Bank at Kirstenbosch
The Leslie Hill Molecular Systematics Laboratory at Kirstenbosch, South Africa
Bank at the National Herbarium Nederland
For systematic treatments (Flora) in the National Herbarium of Netherland
cDNA clones, RFLP markers, PAC/BAC clones and YAC filters
NIAS DNA Bank
For maintaining DNA materials and information that has been accumulated as part of the genome projects such as the
Rice Genome Research Program (RGP)

9. Other DNA banks world-wide …1
Riken Bio-resource Center DNA Bank
Isolation, collection, preservation and distribution of cloned DNA and gene libraries (cDNA and genome
libraries) from human and other mammalian cells and from microorganisms (vectors and hosts). The RIKEN
DNA Bank undertakes research to ensure the authenticity of the materials in the collection and to improve and
standardize the methods of characterization, maintenance, preservation and distribution of genetic resources
San Francisco Zoo DNA Bank
The Zoo maintains a bank with hair, feather or other tissue samples
The Frozen Ark
The mission of the Frozen Ark Project is to collect, preserve and store DNA and viable cells from animals in
danger of extinction
The Ambrose Monell Cryo Collection, New York
In the American Museum of Natural History; approximately one million frozen tissue samples representing the
DNA of a wide range of species

10. Other DNA banks world-wide …2
The Animal Gene Storage Resource Centre of Australia
To preserve reproductive cells, (semen, embryos, ovaries et cetera) and genetic material in a frozen state, at -
196°C in liquid nitrogen
Conservation Genome Resource Bank for Korean Wildlife
Tissues, blood, DNA, somatic and germ cells, and semen from mammals, birds, amphibians, and reptiles
including endangered species of Korea
San Diego Zoo
Conservation by DNA Barcoding for identifying species from unrecognizable samples of blood, bone, meat, hair,
feathers, or feces.
Austrian DNA Bank for Farm Animal Genetic Resources
To secure preservation-worthy farm animal races
National Plant, Fungi and Animal DNA Bank, Poland
Initiative of five Polish scientific institutions using DNA barcoding for researching as well as for many practical
purposes

11. Source of genomic resources
A. Mega-projects
i. Genome sequencing
ii. Allele mining and bio-prospecting
B. On-going projects in NARS on gene isolation/cloning/
expression, etc.
C. On-going projects in CSIR/DBT/University/others
D. Material originating outside India

12. II. Modalities of operation
i. Submission form and MTA
ii. Request form and MTA
iii. Research output form
iv. Quality and quantity of the material
v. Minimum data accompanying the material

13. i. Submission form and MTA
II. Modalities

14. ii. Request form and MTA
II. Modalities

15. iii. Research output form
II. Modalities

16. iv. Quality and quantity of the material
• Genomic DNA
▫ A260/A280=1.7-2.0; A260/A230>1.5
▫ Agarose gel electrophoresis photo with 
▫ >50ng/, > 10, 10 replications
• Library
▫ <5% empty vectors
▫ Free from all sorts of contaminations
▫ 384 well plate (BACs), 96 well plate (cDNA, shotgun, EST)
▫ 1 library  192 plates or  one filter (36,884 spots)
▫ Amplified library  1010 pfu/ml; at least 106 pfu/ml
II. Modalities

17. v. Accompanying data
II. Modalities
Genomic DNA
Name of the plant
Genus
species
common name
Family
TaxID
Genebank Accn. No.
Extraction procedure
DNA dissolved in
Concentration (ng per microL)
Importance of the plant
Reasons for the choice of the
variety/landrace/accession
Source of the biological material
Clear from MTA for plant material?
Any ITK
Any publications? (pl. attach a copy)
Cloned DNA
Name of the plant
common name
Family
TaxID
Genebank Accn. No.
Vector, antibiotic marker
Cloning sites, fragment size (range)
Bacterial strain, transformation method
Culture conditions, Storage conditions
Importance of the plant
Reasons for the choice of the variety/landrace/accession
Source of the biological material
Clear from MTA for plant material?
Any ITK
Any publications? (pl. attach a copy)

18. RT
4°C
-20°C
-80°C
-196°C
Research output form
MTA
Request form
Information
Accession No.
Submission form
Agreement, benefit sharing
MTA
Reposition (quality,
quantity, purity, data)
Distribution
Aliquot
Replicate
Recipient
Donor

19. Intellectual property and legal issues
• Material transfer agreement (MTA) by the beneficiary prior to
the shipment
• The MTAs regulate the intellectual property rights (IPR) of the
requested material and related information, the conditions of
its use and distribution to third parties, as well as benefit
sharing
• Existing MTAs can be specifically designed for the exchange of
genomic resources, if desired
• DNA exchange through MTA: CATIE, Costa Rica; NIAS, Japan,
the Missouri Botanical Garden, USA; and the Royal Botanic
Gardens, Kew

20. Genomic resources bank is a reality
and a need of the hour
• The accelerated use of genomic technology in
biological research has fueled the need to
establish genomic resources bank
• Short-term collections, where newly obtained
material is characterized and distributed based
on current goals
• Long-term collections, where samples are placed
in safe-keeping

21. Facilities for research and generation
of genomic resources at NBPGR

22. Status of Genomic Resources holdings
at NBPGR
S.
No.
Category 2010
-11
2011
-12
2012
-13
2013
-14
31.5. 2014
1 DNA Fingerprinting 0 20 20 66 2281
2 Genomic Resources
A Markers 68 0 4247 40 4355
B Genomic DNA
Varieties
0 55 90 375 3692
C BAC Clones 0 0 0 0 386000
D Trait/gene specific
clones
0 0 0 0 48
3 Utilization of Genomic
resources- markers
0 1256 40 13 1269

23. Genomics Platform: Objectives
 Generate genomic resources for every
prioritised crop
 Develop computational biology tools
 Advance plant systems biology
 Translate basic discovery to the field
 Capacity building, HRD and trainings
 Develop plant genome database
 Strengthen Repository of Genomic Resources

24. PGR
conservation
at NBPGR
NABG
Genomics
Platform
Genomics
Resources
Repository
Phenomics facility Allele mining
Other genomics
projects
Marker assisted
genetic
improvement

25. Repository and
Utilization platform
High throughput
Genomics platform
High throughput
Transcriptomics
platform
Bioinformatics
platform
National Genomic
Resources Repository
Generation of genomic resources by de
novo sequencing, targeted re-sequencing
of indigenous genetic resources
Gene discovery, allele mining and bio-
prospecting in indigenous genetic
resources
Single window system for
assemblage, conservation, exchange
and IPR issues
Computational systems, tools and
expertise for assembly, analysis,
mining, comparative studies

26. Utilisation of Genomic Resources
CROP IMPROVEMENT
 Markers: Molecular Breeding
 Genes: Transgenic development
 Allele mining and mutant generation
 Assessing plant diversity
 Comparative genomics
 Understanding epigenomes
 Genotyping

27. ICAR Genomics
Platform
Fish GR Insect GR Plant GR Animal GR Microbe GR
National Genomic Resources Repository
De novo sequencing, Re-sequencing, High throughput genotyping and
Development of QTLs and markers for priority traits
(Genes, ESTs, cDNAs, Markers, Gene constructs)
Value added genomic resources
for molecular breeding
New genes and constructs for
genetic engineering

28. Thank you
Genomic resources bank is a reality and need of the hour