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Gram-Negative Bacilli Infections: Vibrios, Pseudomonas, Campylobacter, Helicobacter, Haemophilus, Bordetella

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MD Specialclass
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[object Object],Other gram-negative bacilli
VIBRIOS ,[object Object],[object Object],[object Object],[object Object],[object Object]
Vibrio cholerae ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Vibrio cholerae ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Vibrio cholerae
Vibrio cholerae Cholera Toxin B subunit A subunit Binds GM1 ganglioside (surface of epithelial cells) Carried to ER (retrograde transport) Endocytosis Reduced by protein disulfide isomerase in ER Cytosol Unfolding Refolding Interact with cytosolic ADP ribosylation factors Activate G protein Stimulate adenylate cyclase Inc. cAMP Open CFTR Cl released in lumen; secretion of HCO 3 , Na + & water
Vibrio cholerae ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Vibrio cholerae ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Growth on TCBS agar plate
Vibrio parahaemolyticus ,[object Object],[object Object],[object Object],[object Object]
 
Vibrio vulnificus ,[object Object],[object Object],[object Object],[object Object]
 
Pseudomonas aeruginosa ,[object Object],[object Object],[object Object],[object Object]
Pseudomonas aeruginosa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Pseudomonas aeruginosa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Pseudomonas aeruginosa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
Ecthyma gangrenosum
 
Osteochondritis of foot
 
Pseudomonas aeruginosa ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Production of pyocyanin, water-soluble green pigment of Pseudomonas aeruginosa. (left tube)
Campylobacter jejuni ,[object Object],[object Object],[object Object],[object Object],[object Object]
 
Campylobacter jejuni ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Campylobacter jejuni ,[object Object],[object Object],[object Object],[object Object]
Helicobacter pylori ,[object Object],[object Object],[object Object]
 
 
 
 
Helicobacter pylori ,[object Object],[object Object],[object Object],[object Object]
Helicobacter pylori ,[object Object],[object Object],[object Object]
 
 
Helicobacter pylori ,[object Object],[object Object],[object Object],[object Object]
HAEMOPHILUS ,[object Object],[object Object],[object Object],[object Object],[object Object]
Haemophilus influenzae (Pfeiffer’s Bacilli) ,[object Object],[object Object]
Haemophilus influenzae ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Haemophilus influenzae: Culture ,[object Object],[object Object]
Haemophilus influenzae Growth Characteristics: ,[object Object],[object Object]
Haemophilus influenzae Characteristics & Growth Requirements: Species X Factor V Factor Hemolysis H. influenzae H. parainfluenzae H. ducreyi H. haemolyticus H. parahaemolyticus H. aprophilus + - + + - - + + - + + - - - - + + -
Haemophilus influenzae: Virulence Factors ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],Haemophilus influenzae: Virulence Factors
Haemophilus influenzae: Clinical Features ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Haemophilus influenzae: Clinical Features
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Haemophilus influenzae Clinical Features: Sepsis with gangrene
Haemophilus influenzae: Prevention ,[object Object],[object Object],[object Object],[object Object]
Haemophilus aegypticus ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Haemophilus ducreyi ,[object Object],[object Object],[object Object],[object Object],[object Object]
Haemophilus ducreyi
Bordetella pertussis ,[object Object],[object Object],[object Object],[object Object]
Bordetella pertussis ,[object Object],[object Object],[object Object],[object Object]
Bordetella pertussis Gram stain Culture on chocolate agar
Bordetella pertussis: Virulence Factors ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bordetella pertussis: Virulence Factors
[object Object],[object Object],Bordetella pertussis: Pathogenesis
[object Object],[object Object],[object Object],[object Object],Bordetella pertussis: Pathogenesis
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],Bordetella pertussis: Diagnosis
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
BRUCELLA ,[object Object],[object Object],[object Object],[object Object]
BRUCELLA ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]
Species Animal Pathology B. melitensis Goats Acute and severe infection B. suis Swine Chronic with suppurative lesions; caseating granulomas B. abortus Cattle Mild disease without suppuration; Non-caseating granulomas of the RES (LN, liver, spleen, BM) B. canis Dogs Mild disease
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object]

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Gram-Negative Bacilli Infections: Vibrios, Pseudomonas, Campylobacter, Helicobacter, Haemophilus, Bordetella

  • 1.
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  • 6. Vibrio cholerae Cholera Toxin B subunit A subunit Binds GM1 ganglioside (surface of epithelial cells) Carried to ER (retrograde transport) Endocytosis Reduced by protein disulfide isomerase in ER Cytosol Unfolding Refolding Interact with cytosolic ADP ribosylation factors Activate G protein Stimulate adenylate cyclase Inc. cAMP Open CFTR Cl released in lumen; secretion of HCO 3 , Na + & water
  • 7.
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  • 9. Growth on TCBS agar plate
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  • 24. Production of pyocyanin, water-soluble green pigment of Pseudomonas aeruginosa. (left tube)
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  • 44. Haemophilus influenzae Characteristics & Growth Requirements: Species X Factor V Factor Hemolysis H. influenzae H. parainfluenzae H. ducreyi H. haemolyticus H. parahaemolyticus H. aprophilus + - + + - - + + - + + - - - - + + -
  • 45.
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  • 52. Haemophilus influenzae Clinical Features: Sepsis with gangrene
  • 53.
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  • 59. Bordetella pertussis Gram stain Culture on chocolate agar
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  • 71. Species Animal Pathology B. melitensis Goats Acute and severe infection B. suis Swine Chronic with suppurative lesions; caseating granulomas B. abortus Cattle Mild disease without suppuration; Non-caseating granulomas of the RES (LN, liver, spleen, BM) B. canis Dogs Mild disease
  • 72.
  • 73.