2. Sphingolipid and cholesterol cause
distinct phase to form in pure lipid
mixtures containing phospholipid
with
saturated fatty acid chains
Mix DOPC (unsat’d) or DPPC (sat’d) at different ratios,
with sphingolipid +/- cholesterol, at 23oC or 37oC. Assay
by fluorescence quenching of marker that only sees sol
phase lipid. Phase separation indicated by break in slope
of quenching curves.
See 2nd phase at 23oC with increasing DPPC
.
Unsaturated fatty acids inhibit 2nd phase.
Cholesterol promotes 2nd phase formation
Need cholesterol to obtain 2nd phase at 37oC.
3. ”Nesten hver eneste
PowerPoint
presentasjon stinker
som et råttent egg”
Seth Godin
32. Story Board
Design
Title & Story Storyboard Cluttered
Principle Bad Graph
Intro Frames Images Image
1
Design Design
Good Graph 2 Text Text 3 Text
Design
Text Text
4
43. 1-7-7 regelen –Hva er det?
• Ha bare hovedsakelig én idé pr slide
• Sett inn bare syv linjer tekst max
• Bruk bare syv ord pr linje max
• Spørsmålet er – er dette mest effektivt?
• Er denne metoden virkelig et godt råd?
• Er dette virkelig en passende, effektiv
”visuell”?
• Denne ene sliden har bare syv kulepunkter!
44.
45. COGNITIVE
LOAD
THEORY
(Prof. John Sweller, University of NSW)
46. kognitiv load
= den mengden
arbeid som kreves
for å forstå eller
lære noe