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Or the algae in the sea ?
Or the
cacti in the
desserts ?
How does trees on the fertile plain obtain their nutrients ?
Review QuestionReview Question
• Which mode of nutrition do the green
plants carry out?
A. Autotrophic nutrition
B. Heterotrophic nutrition
Sorry! You’re wrong!Sorry! You’re wrong!
• Heterotrophic nutrition is the mode of
nutrition in which organisms have to depend
on other organisms or dead organic matters
as their food sources. Green plants, however,
can make organic food by themselves using
simple inorganic substances.
Very Good!Very Good!
• Autotrophic nutrition is the mode of
nutrition in which organisms can make
organic food by themselves using simple
inorganic substances.
• The process by which the green plants
obtain nutrients is called :
Photosynthesis
Nature of photosynthesisNature of photosynthesis
• Takes place in Chloroplast
• Necessary factors :
Carbon dioxide
Water
Sunlight
Chlorophyll
Route of Carbon DioxideRoute of Carbon Dioxide
for Photosynthesisfor Photosynthesis
CO2
Stoma
Air Space
Spongy Mesophyll
Cell
Chloroplast
CO2
Chloroplast
Palisade Mesophyll
Cell
Stoma
Air Space
 Light energy is trapped by chlorophyll in
chloroplast
 Light energy absorbed by chlorophyll splits
water molecules into hydrogen and oxygen
 Oxygen is released as a gas through stoma
to outside
 Hydrogen is fed into dark reaction
Light reactionLight reaction
blue red
%oflightabsorbe
chlorophyll
green
66
Oxygen is produce as the by-product of photosynthesis.
Dark reactionDark reaction
 No light is required; can take place either
in light or darkness
 Hydrogen produced in light reaction
combines with CO2 to form carbohydrates
 Water is formed as a by-product
Dark Reaction
Water
Oxygen Glucose
(C6H12O6)
Light Reaction
H
Summary of PhotosynthesisSummary of Photosynthesis
6 CO2 + 6 H20 C6H12O6 + 6 O2
Light Carbon Dioxide
Fate of carbohydrateFate of carbohydrate
products in the plantproducts in the plant
carbohydrates (e.g. glucose)
carbon dioxide and water
photosynthesis
release energy by respiration
convert into starch for storage because
glucose is not a very good storage molecule
carbohydrates (e.g. glucose)
carbon dioxide and water
photosynthesis
change into sucrose and is transported
to other parts through phloem
carbohydrates (e.g. glucose)
carbon dioxide and water
photosynthesis
carbon dioxide and water
photosynthesis
carbohydrates (e.g. glucose)
fatty acids glycerol
combine to form fats and oils to form cell
membranes and as a food store
carbon dioxide and water
photosynthesis
carbohydrates (e.g. glucose)
mineral salts from soil
(e.g. NO3
-
, SO4
2-
)
amino acids
join together to become
protein molecules
Experiments onExperiments on
photosynthesisphotosynthesis
DestarchingDestarching
• Reason:
– To avoid any existing starch in the leaves
interfering with the result, and to show that
any starch found after the experiment is
produced during the period of
investigation
• Method:
– By placing the plant in dark for at least 48
hours
To test a leaf for the presence
of Starch
Why is the leaf first boiled in water ?
Ans: Because the process can soften the leaf, break
down the cuticle and kill the leaf.
boiling
water
turn off
Bunsen
burner
boiling
alcohol hot water
iodine
white tile
turn off
Bunsen
burner
Why is the leaf then boiled in alcohol ?
Ans: To decolourize the leaf (to remove chlorophyll).
boiling
water
turn off
Bunsen
burner
boiling
alcohol hot water
iodine
white tile
turn off
Bunsen
burner
Why is it important to turn off the Bunsen burner when you
are heating the alcohol ?
Ans: Because alcohol catches fire easily.
boiling
water
turn off
Bunsen
burner
boiling
alcohol hot water
iodine
white tile
turn off
Bunsen
burner
Why is the leaf put in hot water after being boiled in
alcohol ?
Ans: Since the leaf becomes brittle after boiling in
alcohol, so dipping it into hot water enables it to be
softened.
boiling
water
turn off
Bunsen
burner
boiling
alcohol hot water
iodine
white tile
turn off
Bunsen
burner
What colour change can be observed if starch is present ?
Ans: The leaf becomes blue-black when iodine solution
is added to it.
boiling
water
turn off
Bunsen
burner
boiling
alcohol hot water
iodine
white tile
turn off
Bunsen
burner
To show that Chlorophyll is
necessary for Photosynthesis
Which part of the leaf turns blue-black when treated with
iodine solution ?
Ans: Only the green part.
green part
purple part
Is chlorophyll necessary for photosynthesis ?
Ans: Yes.
green part
purple part
What is the control in this experiment ?
Ans: The green part of the leaf acts as the control.
green part
purple part
To show that Carbon
Dioxide is necessary for
Photosynthesis
Why is potassium hydroxide solution put in one of the
flasks ?
Ans: To absorb all the carbon dioxide in flask B.
sunlight
B
A
water
potassium
hydroxide solution
What happens to each leaf after testing the presence of
starch at the end of the experiment ?
Ans: The leaf in flask A changes to blue-black while the
leaf in flask B stains brown.
sunlight
B
A
water
potassium
hydroxide solution
What do your results suggest about the relationship
between carbon dioxide and photosynthesis ?
Ans: Carbon dioxide is necessary for photosynthesis.
sunlight
B
A
water
potassium
hydroxide solution
Factors affectingFactors affecting
the rate ofthe rate of
photosynthesisphotosynthesis
 Light intensity
 Rate of Photosynthesis
 Carbon dioxide
concentration
 Rate of Photosynthesis
 Temperature
 Rate of Photosynthesis
Temperature > 40℃
Rate  & Stop soon
If no water, stomata close
no gaseous exchange
photosynthesis ceases
Why is PhotosynthesisWhy is Photosynthesis
important ?important ?
Green plants: the only organisms
capable of trapping light
to manufacture food from
simple inorganic
substances
Green plants(producers) starts the
food chain & provide food for other
organisms
Why is PhotosynthesisWhy is Photosynthesis
important ?important ?
Atmospheric carbon dioxide is absorbed
& oxygen is released into the atmosphere
which maintains the composition of the
atmosphere constant
Why is PhotosynthesisWhy is Photosynthesis
important ?important ?
Parts of plant whereParts of plant where
photosynthesis takes placephotosynthesis takes place
Mainly in the leaf because
–it contains a lot of chloroplasts
–it is well adapted for performing
photosynthesis
InternalInternal
Structure of LeafStructure of Leaf
cuticle
upper epidermis
palisade mesophyll
spongy mesophyll
intercellular space
(air space)
guard cell
lower epidermis
cuticle
stoma
chloroplasts
Cross-sectionCross-section
of a leaf ofof a leaf of
dicotyledondicotyledon
upper
epidermis
protect internal
tissues from
mechanical
damage and
bacterial &
fungal invasion
Cuticle
• a waxy layer
• prevent
water loss
from the leaf
surface
• protect the
leaf
mesophyll
palisade
mesophyll
(layer)
columnar cells
closely packed
together
∴absorb light
more efficiently
contains many
chloroplasts
spongy
mesophyll
(layer)
irregular cells
loosely packed
together to leave
numerous large air
spaces
→allow rapid
diffusion of gases
throughout the leaf
some (fewer
than palisade
mesophyll)
chloroplasts for
photosynthesis
lower
epidermis
same as
upper
epidermis
except the
cuticle is
thinner
stomastoma
(stomata)(stomata)
opening which
allows gases
to pass
through it to go
into or out of
the leaf
guard cellsguard cells
control the
size of stoma
StomaStoma
stoma
Guard cells
thinner outer wall
thicker inner wall
vascular
bundle
(vein)
Internal Structure of LeafInternal Structure of Leaf
xylem
phloem
xylemxylem
• to transport water
and mineral salts
towards the leaf
• for mechanical
support
phloemphloem
• to transport
organic
substances
(food/sugar/
glucose/
sucrose) away
from the leaf
Adaptation of leafAdaptation of leaf
to photosynthesisto photosynthesis
The leaf is thin
decrease diffusion
distance for gases &
light
The leaf is broad &
flattened
increase surface area
to absorb more
sunlight
Cuticle exists in
upper epidermis and
is transparent
Allows most light to
pass into
photosynthetic
mesophyll tissues
Palisade mesophyll
cells are closely
packed and contain
many chloroplasts
To carry out
photosynthesis more
efficiently
Spongy mesophyll
cells are loosely
packed with numerous
large air spaces
To allow rapid diffusion
of gases throughout
the leaf
Numerous stomata
on lower epidermis
To allow rapid
gaseous exchange
with the
atmosphere
Extensive vein system
• Allow sufficient
water to reach the
cells in the leaf
• To carry food away to
other parts of the
plant
Mineral requirementsMineral requirements
in plantsin plants
• In order to synthesize amino acids, nitrate ions
which must be taken into the plant from the soil
through the root
• Other minerals are also necessary to maintain
the life of the plant (N, Mg, P, K, S, etc)
The importance ofThe importance of nitrogennitrogen
• For synthesis of proteins, chlorophyll, etc
• Taken in form of nitrate ions
• Deficiency symptoms:
– Little growth ( - no protein made)
– Yellowing of leaves ( - no chlorophyll
made)
The importance ofThe importance of magnesiummagnesium
• Essential component of chlorophyll
• Deficiency symptoms:
– Yellowing of leaves (no chlorophyll made)
– Poor growth (no food manufactured
because of lack of chlorophyll)
Use of fertilizers inUse of fertilizers in
agricultureagriculture
• Continuous harvesting crops removes
valuable mineral salts from soil
∴Fertilizers are added to replace such loss
• Two kinds of fertilizers:
– Natural fertilizers
– Chemical fertilizers
Natural fertilizersNatural fertilizers
• From manure
• Organic compounds in it are
decomposed by the bacteria in
soil to form mineral salts
Chemical fertilizersChemical fertilizers
• Mainly nitrogenous and phosphorous
compounds manufactured artificially
Comparison between naturalComparison between natural
and chemical fertilizersand chemical fertilizers
Natural fertilizers Chemical fertilizers
Contain humus which can
improve soil texture
No humus so cannot improve
soil texture
Less soluble in water so less
likely to be washed away
Very soluble in water so more
likely to be washed away
Comparison between naturalComparison between natural
and chemical fertilizersand chemical fertilizers
Natural fertilizers Chemical fertilizers
Less soluble in water so more
difficult to be absorbed
Very soluble in water so
easier to be absorbed
More readily to be used by
the plants
Time is needed for the
decomposition to complete
before nutrients are available
to plants
Very expensiveMuch cheaper

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