1. Dr.Bernaud Rimland
Authored the Neurobiological
approach.
The First
POSITIVE TURN IN
AUTISM RESEARCH
Four decades of Global Research.
Are we able to deliver peace at home?
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2.  Autism research exhibits analytical
threads, but patterns remain
fragmented lacking a unified concept.
As on date we lack a coherent
view on brain function within the
framework of other organ
systems.
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3. POSITIVE APPROACH of
DOAST
„Instead of studying it through a
symptom by symptom approach,
autistic behavior should be
understood better by studying the
underlying intermediary biological
processes‟
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4. Emerging qualitative changes in
clinical status including loss of
diagnosis suggest that a more
appropriate description for ASD could
be a “chronic dynamic reversible
encephalopathy” rather than “static
encephalopathy” as considered so far.
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5. “Cognitive, emotional and synaptic
functions of the brain basically rely
on the macromolecular computing
system of RNA protein- and protein
to protein interactions which appear
to be the result of fine grained
information, encoded and
accumulated over evolutionary
time”.
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6. Neuro-Endo-Immune-
GI Tract Network,
Autonomic Balance,
&Neurotransmitters.
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7. Genes, CNS-ENDO-IMMUNE-GI
Molecular order,
Bio feed back &
Tract- Network
Protein expression, Autonomic Balance
Homeostasis
Nerurotransmitters
Neuronal connectivity.
Upstream
molecular
unrest
Altered Normal
network Performance Downstream
Homeostasis Social&Cognitive,
Activities
Systemic
Inflammation
Behavior&Cognitive
dysfunction. Susceptible
Genetic
AUTISM landscape
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9. Successful in-vivo technique
in synergy with Global research.
Follow the LEADS and focus on safe
and effective interventions
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11. “Decreased connectivity is at the heart
of autism‟.
Fairly close regions of the brains involved in
motor tasks have difficulty coordinating
activity.
It makes sense that social and communication
skills are greatly impaired, as they involve
more complex synchronous coordination
between more distant areas of the brain.
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12. Consequences of inflammatory factors
Breaking boundaries between the body
and brain,
Destabilization and loss of synaptic
connections.
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13. Our Concept.. Upstream
molecular
unrest
Down stream
inflammation
Dietary or environmental
triggers,
Synaptic
Gut micro biome
systemic inflammatory connectivity
cytokines on a chronic basis affected in in
susceptible
individuals.
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14. Genes, CNS-ENDO-IMMUNE-GI
Molecular order,
Bio feed back &
Tract- Network
Protein expression, Autonomic Balance
Homeostasis
Nerurotransmitters
Neuronal connectivity.
Upstream
molecular
Normal order
Performance
Social&Cognitive,
Restore Activities
Network Downstream
Homeostasis Molecular
order
INTEGRATED
THERAPY
Improved
Behavior& Cellular
Cognition Stressors
Integrated Therapy Hypothesis
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15. Deviate from the „Hard wired‟ concept and „untreatable‟ stigma.
Effectively treating the „downstream
inflammatory pathology‟ could initiate
reestablishment of „upstream molecular
order‟ . Molecular order restored could
activate the dormant neuronal pools,
improve connectivity and restore
functional integration.
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16. Integrated therapy
Pioneering concept of therapeutic integration
or fusion, where scientifically chosen
traditional Indian treatment methods are
applied with modern medical understanding
to treat specific chronic ailments, and autism
in particular.
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17. The Components of Integrated Therapy.
The Science of Yoga, Ayurveda &
Siddha &
Modern medical expertise.
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18. Reducing Cellular stress
Toxin elimination
Rejuvenation and
Regeneration.
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19. Prognostic Evaluation
No reliable bio-marker.
Observed improvements in
behavior and cognition domains are
the only reliable non-invasive
indicators of restoration of
molecular order and integration of
the upstream neuronal pools.
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20. Patient selection.
• DSM-IV guidelines, (CARS) and M-CHAT are
the standard assessment tools used.
• Patient pool represents a spectrum of severity
observed in autistic patients in general.
• Their racial origin is Asian. Age 3- 16 years.
• Cohort of 30 individuals on different
follow up period on this therapy since
2004.
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21. Therapeutic protocol
 The prescribed diet „Sathvik Diet‟
practiced by the science Yoga.
 Herbal medications, certified by the
Ayurvedic [including Siddha] pharmacopia
of India are administered.
 Yoga technique is a combinatorial
approach.
 Oleation therapies with medicated oils.
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22. Ethical & safety guidelines followed
 A well informed consent,
 The therapy techniques and medicines are chosen
by evidence based risk benefit ratio, proven over
centuries in their clinical application,
 One to one & Group basis application along with
the parent,
 Documentation on paper and video,
 Pretreatment EEG and periodic monitoring of
biochemical parameters to assess renal, hepatic
and hematological functions.
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23. Assessment of Clinical benefits
GI Tract, Eye contact,
Play,
Respiratory,

Communication,
Sleep,  Empathy
Morbidity, & Generalization
are the behavior domains
Emotions,
chosen to assess and
Behavior, quantify behavior and
Cognition. cognitive improvement.
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24. Controls
 We follow each patient, from the time of admission and
through their recovery (or lack of it). Thus each participant
in the study will serve as his/her own control.
 Improvement is influenced by the age of induction into
therapy, duration of follow-up and compliance to the details.
Parental compliance to be proportional to the trust on
therapy.
Willful or accidental default in medicine or dietary regime
'influenced by the above listed „variables', resulted in
temporary return of symptoms. Such events helped us
understand the therapeutic role played by components of
therapy in a better way.
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25. VALIDITY
 Consistent replication lends validity,
 Supports the hypothesis that chronic
inflammation could indeed be the common
denominator.
Future research
We contemplate generating carefully
characterized full genome expression profiling
data to validate our biomedical approach and to
study the involvement of the intestinal flora in
the inflammation related etiology of autism.
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26. The core of our study will be a repeated
measures examination of the interplay
between the biology of inflammatory and
oxidative stress markers on the one hand
and processing, connectivity and reactivity
abnormalities on the other which may
conceivably converge on to the autism
profile.
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27. Proposed Study
 Examine the gastrointestinal
metagenomic analysis of intestinal
flora,
Non-coding RNA (ncRNA) expression
profiles on lymphoblasts in a series of
before and after comparison based
molecular studies.
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28. Outcome of the proposal
Generate direct evidence in favor of a
causal relationship between systemic
inflammation and autism,
Generate diagnostic determinants for
definition of this variant of autism,
Define a link between diagnostics and
treatment by this and any future treatment
technique.
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29. In the least invasive manner, using this
DOAST treatment modality as a
physiological probe, we study the linear,
causal relationship between cellular-level
changes and behavioural problems within
the high performance environment of the
human nervous system.
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30. The identification of relationships between
the multiple levels, all of which can be
expected to shift based on both huge
amounts of clinical experience and a
growing wealth of published scientific
data, will be a contribution to identifying
treatment targets that simultaneously
address multiple features of autism at their
root.
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31. While there are risks associated with any dogma
challenging proposal, the most compelling gains
of this project is that success in any of the
multiple facets of this investigation will lead to
important new knowledge about epigenetic and
molecular denominator based integrated
therapeutic approaches for a heterogeneous
neural disorder that has so far not responded
significantly to mono therapy.
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