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PUBLIC DISCLOSURE OF JATROPHA
   BREEDING INFORMATION

             Bionic Palm Ltd., Accra, Ghana, is disclosing the ownership
             of a male-sterile Jatropha plant which is a direct descendant
             from seeds collected from a wild Jatropha curcas L. plant in
             the Southwest of Guatemala
             Responsible for the content: Ulrich B Riemann, MD Bionic Palm Ltd.
             Email: info@bionic-palm.com
             Bionic Palm Limited: bionic-palm.com
             Jatropha Breeder by Bionic Palm: jatropha.bionic-enterprises.com




© COPYRIGHT 2013 BIONIC PALM LIMITED                                              29/01/2013   1
MOTIVATION FOR THIS DISCLOSURE
       Patent application EP2521438 by SG Biofuels attempts to protect a
          generic „female only (FO)“ trait in Jatropha curcas L. (JcL)
       The same patent application is trying to protect an absolutly standard
          general method for hybrid seed production in its use for JcL
       This disclosure proves that male sterility or a so called „FO trait“ occurs
          naturally in JcL, at least in one accession picked from the wild in Guatemala
       Bionic Palm is in possesion of that accession and using it actively in its
          breeding efforts for improved JcL
       Bionic Palm is determined to keep Jatropha development as open as
          possible for all, as the only sound possibility to make significant plant
          enhancement possible in the short term.




© COPYRIGHT 2013 BIONIC PALM LIMITED                                                  29/01/2013   2
FROM SEED COLLECTION TO DISCOVERY
     OF THE MALE-STERILE TRAIT
       Seeds were collected from a single wild plant in Southwestern
          Guatemala (exact coordinates are available to BPL)
       Seedlings were raised in Europe
       A clone (cutting) was transferred to Ghana 11/2011
       First flowering occured in 4/2012
       Second flowering fruits have been harvested
       Third & forth flowering is currently ongoing




© COPYRIGHT 2013 BIONIC PALM LIMITED                                    29/01/2013   3
PICTURE SERIES OF INFLORESCENSE DEVELOPMENT




© COPYRIGHT 2013 BIONIC PALM LIMITED   29/01/2013   4
PROCESS OF MALE FLOWER „CRIPPLING“

     Occured that way without exception
                P1: crippling starts, most male & all female flower buds seem intact


                P2: all male buds clearly crippled, female buds doing fine


                P3: after fuit development started crippled male buds still around


                P4: fruits almost ripe, crippled male buds unchanged




© COPYRIGHT 2013 BIONIC PALM LIMITED                                            29/01/2013   5
P1: MOST BUDS SEEM FINE




© COPYRIGHT 2013 BIONIC PALM LIMITED   29/01/2013   6
P2: ALMOST ALL MALE BUDS ARE DEAD
     (5 DAYS AFTER THE LAST PICTURE, SAME INFLORESCENSE)




© COPYRIGHT 2013 BIONIC PALM LIMITED                       29/01/2013   7
P3: CRIPPLED MALE BUDS STILL AROUND




© COPYRIGHT 2013 BIONIC PALM LIMITED   29/01/2013   8
P4: ...AND THEY ARE STILL VISIBLE IN THE END




© COPYRIGHT 2013 BIONIC PALM LIMITED   29/01/2013   9
ANOTHER MALE STERILE FROM OUR
     PARENTAL ACCESSIONS
                                                  For 2 years and many dozens of
                                                  inflorescenses this and a sister
                                                  plant have consistently not opened
                                                  any male flowers.
                                                  The behavior is however completely
                                                  different: Female flowers open first
                                                  while male buds are still looking good.
                                                  At a later stage (days to weeks after
                                                  pollination of the female flowers)
                                                  the male buds turn yellow and fall
                                                  off completely.


     This plant is a direct descendant from seeds collected from a wild plant
     in Mexico. Only some of the seeds taken from this very plant produced a male
     sterile line, while the others are completely fertile which makes the family
     extremely interesting for breeding tests. Even more interesting, the male steriles
     are toxic (a trait to be reconfirmed) while the fertiles are non-toxic on PE.
© COPYRIGHT 2013 BIONIC PALM LIMITED                                            29/01/2013   10
PUBLIC DISCLOSURE OF JATROPHA
   BREEDING INFORMATION BY BIONIC
   PALM LTD., ACCRA, GHANA

             Please address questions you might have regarding
             this disclosure via email to the below address:


             Email: info@bionic-palm.com

             More about our work can be found on our websites:
             Bionic Palm Limited: bionic-palm.com
             Jatropha Breeder by Bionic Palm: jatropha.bionic-enterprises.com




© COPYRIGHT 2013 BIONIC PALM LIMITED                                            29/01/2013   11

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Public disclosure of a male-sterile Jatropha curcas trait found in wild accessions

  • 1. PUBLIC DISCLOSURE OF JATROPHA BREEDING INFORMATION Bionic Palm Ltd., Accra, Ghana, is disclosing the ownership of a male-sterile Jatropha plant which is a direct descendant from seeds collected from a wild Jatropha curcas L. plant in the Southwest of Guatemala Responsible for the content: Ulrich B Riemann, MD Bionic Palm Ltd. Email: info@bionic-palm.com Bionic Palm Limited: bionic-palm.com Jatropha Breeder by Bionic Palm: jatropha.bionic-enterprises.com © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 1
  • 2. MOTIVATION FOR THIS DISCLOSURE  Patent application EP2521438 by SG Biofuels attempts to protect a generic „female only (FO)“ trait in Jatropha curcas L. (JcL)  The same patent application is trying to protect an absolutly standard general method for hybrid seed production in its use for JcL  This disclosure proves that male sterility or a so called „FO trait“ occurs naturally in JcL, at least in one accession picked from the wild in Guatemala  Bionic Palm is in possesion of that accession and using it actively in its breeding efforts for improved JcL  Bionic Palm is determined to keep Jatropha development as open as possible for all, as the only sound possibility to make significant plant enhancement possible in the short term. © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 2
  • 3. FROM SEED COLLECTION TO DISCOVERY OF THE MALE-STERILE TRAIT  Seeds were collected from a single wild plant in Southwestern Guatemala (exact coordinates are available to BPL)  Seedlings were raised in Europe  A clone (cutting) was transferred to Ghana 11/2011  First flowering occured in 4/2012  Second flowering fruits have been harvested  Third & forth flowering is currently ongoing © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 3
  • 4. PICTURE SERIES OF INFLORESCENSE DEVELOPMENT © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 4
  • 5. PROCESS OF MALE FLOWER „CRIPPLING“ Occured that way without exception P1: crippling starts, most male & all female flower buds seem intact P2: all male buds clearly crippled, female buds doing fine P3: after fuit development started crippled male buds still around P4: fruits almost ripe, crippled male buds unchanged © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 5
  • 6. P1: MOST BUDS SEEM FINE © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 6
  • 7. P2: ALMOST ALL MALE BUDS ARE DEAD (5 DAYS AFTER THE LAST PICTURE, SAME INFLORESCENSE) © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 7
  • 8. P3: CRIPPLED MALE BUDS STILL AROUND © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 8
  • 9. P4: ...AND THEY ARE STILL VISIBLE IN THE END © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 9
  • 10. ANOTHER MALE STERILE FROM OUR PARENTAL ACCESSIONS For 2 years and many dozens of inflorescenses this and a sister plant have consistently not opened any male flowers. The behavior is however completely different: Female flowers open first while male buds are still looking good. At a later stage (days to weeks after pollination of the female flowers) the male buds turn yellow and fall off completely. This plant is a direct descendant from seeds collected from a wild plant in Mexico. Only some of the seeds taken from this very plant produced a male sterile line, while the others are completely fertile which makes the family extremely interesting for breeding tests. Even more interesting, the male steriles are toxic (a trait to be reconfirmed) while the fertiles are non-toxic on PE. © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 10
  • 11. PUBLIC DISCLOSURE OF JATROPHA BREEDING INFORMATION BY BIONIC PALM LTD., ACCRA, GHANA Please address questions you might have regarding this disclosure via email to the below address: Email: info@bionic-palm.com More about our work can be found on our websites: Bionic Palm Limited: bionic-palm.com Jatropha Breeder by Bionic Palm: jatropha.bionic-enterprises.com © COPYRIGHT 2013 BIONIC PALM LIMITED 29/01/2013 11