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Enzyme And Metabolism

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ENZYME & METABOLISM
OBJECTIVES: ,[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],ENZYME??
[object Object]
ENZYME AS BIOLOGICAL CATALYSTS: ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
 
MECHANISM ACTION: There are 2 main hypotheses explaining of enzyme action. Lock & Key model Induced - fit model
[object Object],[object Object],[object Object],[object Object],LOCK & KEY MODEL
[object Object],[object Object],[object Object],[object Object]
 
 
 
[object Object],[object Object],[object Object],[object Object],INDUCED-FIT HYPOTHESIS
INDUCED-FIT HYPOTHESIS
[object Object],[object Object],[object Object],INDUCED-FIT HYPOTHESIS
 
[object Object],[object Object],[object Object]
[object Object],[object Object]
 
How do enzymes work? ,[object Object],[object Object],[object Object], 
 
[object Object],[object Object]
 
 
Factors Influencing Enzyme Activity ,[object Object],[object Object]
[object Object],[object Object],[object Object]
 
 
METABOLISM ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],ANABOLISM CATABOLISM
[object Object],[object Object],[object Object],EXERGONIC REACTIONS
[object Object]
 
[object Object],[object Object],ENDERGONIC REACTIONS
 
 
MICHAELIS-MENTEN EQUATION
[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],V o = V max K M
[object Object],[object Object],[object Object],[object Object],[object Object],ENZYME COFACTORS
[object Object],[object Object],[object Object],ENZYME COFACTORS
  Aldehyde group transfer   thiaminpyrophosphate (TPP)   thiamin (B-1)   Methyl group transfer   coenzyme B-12   vitamin B-12   Acetyl group carrier   coenzyme A (CoA)   pantothenic acid   oxidation or hydrogen transfer   flavin adenine dinucleotide (FAD)   riboflavin   oxidation or hydrogen transfer   nicotinamide adenine dinucleotide (NAD + )   niacin   Function   Coenzyme   Vitamin
[object Object],[object Object],[object Object],[object Object],COENZYMES
[object Object],[object Object],[object Object],[object Object]
 
 
[object Object],[object Object],[object Object],ENZYME INHHIBITORS
 
[object Object],[object Object],NON SPECIFIC INHIBITORS
[object Object],[object Object],EXAMPLE:
[object Object],[object Object],[object Object],COMPETITIVE INHIBITORS
[object Object],[object Object],[object Object],COMPETITIVE INHIBITORS
 
 
[object Object],[object Object],[object Object],NON-COMPETITORS INHIBITORS
NON-COMPETITORS INHIBITORS
[object Object],[object Object],[object Object],NON-COMPETITORS INHIBITORS
[object Object],[object Object],[object Object],CLASSIFICATION OF ENZYME
1.Oxidoreductases ,[object Object],[object Object],[object Object]
[object Object],[object Object],oxidases
dehydrogenases ,[object Object],[object Object]
peroxidases ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],oxygenases
2.Transferases ,[object Object],[object Object]
3.Hydrolases ,[object Object],[object Object],[object Object]
4.Lyases ,[object Object]
5.Isomerases ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
6.Ligases ,[object Object],[object Object],[object Object],[object Object]
Formation of bonds with ATP cleavage   Ligases or Synthetases Isomerization reactions   Isomerases Addition to double bonds or its reverse   Lyases Hydrolysis reactions   Hydrolases Transfer of functional groups   Transferases   Oxidation-reduction reactions   Oxidases or Dehydrogenases   Type of Reaction Catalyzed   Group Name IEC Classification of Enzymes
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],PROPERTIES OF ENZYMES
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],PROPERTIES OF ENZYMES
 
[object Object],[object Object],[object Object],[object Object],EXERCISE:

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Enzyme And Metabolism

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  • 8. MECHANISM ACTION: There are 2 main hypotheses explaining of enzyme action. Lock & Key model Induced - fit model
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  • 43.   Aldehyde group transfer   thiaminpyrophosphate (TPP)   thiamin (B-1)   Methyl group transfer   coenzyme B-12   vitamin B-12   Acetyl group carrier   coenzyme A (CoA)   pantothenic acid   oxidation or hydrogen transfer   flavin adenine dinucleotide (FAD)   riboflavin   oxidation or hydrogen transfer   nicotinamide adenine dinucleotide (NAD + )   niacin   Function   Coenzyme   Vitamin
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  • 70. Formation of bonds with ATP cleavage   Ligases or Synthetases Isomerization reactions   Isomerases Addition to double bonds or its reverse   Lyases Hydrolysis reactions   Hydrolases Transfer of functional groups   Transferases   Oxidation-reduction reactions   Oxidases or Dehydrogenases   Type of Reaction Catalyzed   Group Name IEC Classification of Enzymes
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