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In Situ Hybridization (ISH) is a technique that allows for precise localization of a specific segment of nucleic acid within a histologic section.
In situ hybridization
In situ hybridization
Creative-Bioarray
Chromogenic In Situ Hybridization
Cish
Cish
home education
What is in situ hybridization Radioactive ISH Fluorescent ISH Colorimetric ISH ISH: three variables The sample The probe Optimizing ISH Detection ISH controls Data Analysis
In situ hybridization (ISH) analyses
In situ hybridization (ISH) analyses
abizarl
What is Fluorescent in-situ Hybridization (FISH)?
Fluorescent in-situ Hybridization (FISH)
Fluorescent in-situ Hybridization (FISH)
BioGenex
A brief description about three main types of blotting techniques used in biological research
Blotting techniques
Blotting techniques
Gurvinder Kaur
defination, protocol, and some history
Colony hybridization technique
Colony hybridization technique
ShreyaBhatt23
These slides include all the 3 blotting techniques i,e Southern,northern and western.Their principle,procedure and application.
Blotting techniques
Blotting techniques
Farha Banu
Rflp 2513
Rflp 2513
atikaagarwal
Recomendados
In Situ Hybridization (ISH) is a technique that allows for precise localization of a specific segment of nucleic acid within a histologic section.
In situ hybridization
In situ hybridization
Creative-Bioarray
Chromogenic In Situ Hybridization
Cish
Cish
home education
What is in situ hybridization Radioactive ISH Fluorescent ISH Colorimetric ISH ISH: three variables The sample The probe Optimizing ISH Detection ISH controls Data Analysis
In situ hybridization (ISH) analyses
In situ hybridization (ISH) analyses
abizarl
What is Fluorescent in-situ Hybridization (FISH)?
Fluorescent in-situ Hybridization (FISH)
Fluorescent in-situ Hybridization (FISH)
BioGenex
A brief description about three main types of blotting techniques used in biological research
Blotting techniques
Blotting techniques
Gurvinder Kaur
defination, protocol, and some history
Colony hybridization technique
Colony hybridization technique
ShreyaBhatt23
These slides include all the 3 blotting techniques i,e Southern,northern and western.Their principle,procedure and application.
Blotting techniques
Blotting techniques
Farha Banu
Rflp 2513
Rflp 2513
atikaagarwal
Zoonotic pathogens cause diseases and death both in human & animals which ultimately leads to man power and economic loss of the country. Traditional diagnostic methods identify a pathogen based on its phenotype. The correct assessment of a clinical isolate takes more time. Faster and simpler methods of diagnosis is of great advantage. That is why molecular biology technique is the first and foremost choice .
MOLECULAR BIOLOGY TECHNIQUES USED IN ZOONOTIC DISEASE
MOLECULAR BIOLOGY TECHNIQUES USED IN ZOONOTIC DISEASE
Nataraju S M
BLOTTING TECHNIQUES..........
Blotting techniques (manish)
Blotting techniques (manish)
manish chovatiya
Presentation Monica Stoian
Presentation Monica Stoian
Mihaiela Fazacas
It is most important ppt of northern blotting to educate the basic learner of Biotechnology
Northern blot
Northern blot
Aashish Patel
Fluorescent in situ hybridization (FISH) is a cytogenetic technique that uses fluorescent probes to investigate the presence of small, submicroscopic chromosomal changes that are beyond the resolution of karyotype analysis. This PowerPoint presentation explain the concept,process and application of Fluorescence insitu hybridization.
Fluorescence in situ hybridization
Fluorescence in situ hybridization
TukaramBorkar
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH) by Creative Diagnostics, learn more http://www.creative-diagnostics.com/in-situ-hybridization-and-fluorescence-in-situ-hybridization.htm
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH)
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH)
Creative-Diagnostics
Define karyotype and FISH Describe the procedure of karyotyping and FISH Explain chromosomal abnormalities through karyotyping and FISH Describe the principles of FISH Presented by- Dr. Subarna Das Resident, MS Anatomy Phase-A, Year-1, Block-2 Guided by- Prof. Laila Anjuman Banu Chairman Department of Anatomy, BSMMU
Karyotype and FISH
Karyotype and FISH
Rayhan Shahrear
Blotting
Northern, southern and western blotting
Northern, southern and western blotting
Ravi Kant Agrawal
techniques in molecular biology and cell line culturing with animal handelling
Various techniques in molecular bology
Various techniques in molecular bology
Kartikey Singh
Southern northern and western blotting
Southern northern and western blotting
Sana Javed
Blotting technology by using plant materials.
Blotting and its type
Blotting and its type
MonishaB2
A concise not on each of three different blotting technique
Blotting (Southern, Northern and Eastern)
Blotting (Southern, Northern and Eastern)
Aman Ullah
short and sweet description about blotting techniques
Blotting techniques
Blotting techniques
Kashikant Yadav
blotting and their types
Blotting southern,northern, western techniques
Blotting southern,northern, western techniques
veeralxmi
blotting techniques used to identify DNA, RNA and Protein and its Post-modification changes. blotting techniques are of three basis types; southern blotting western blotting eastern blotting
Blotting techniques
Blotting techniques
KinzaHaroon1
Southern Blotting is a technique developed by E.M. SOUTHERN BLOTTING in 1975. It includes Principle, steps and applications of southern blotting.
Southern blotting
Southern blotting
HariniV39
CHROMOSOME PAINTING: A METHOD FOR CELL CHARACTERIZATION
Chromosome painting
Chromosome painting
Prince Allawadhi
Molecular technique presentation by Dev vrat shukla
Fluorescent in situ hybridization technique
Fluorescent in situ hybridization technique
Devvrat Shukla
most effective slide for the medical student ,though you will find most of the things in short time..
Blotting techniques
Blotting techniques
Kashikant Yadav
blotting techniques
Blotting techniques
Blotting techniques
Alex Chris
in suite hybridization (objective, applications, protocol)
Insuite hybridization
Insuite hybridization
Noman Ch
molecular breeding
Fish
Fish
Shreya Lodh
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Zoonotic pathogens cause diseases and death both in human & animals which ultimately leads to man power and economic loss of the country. Traditional diagnostic methods identify a pathogen based on its phenotype. The correct assessment of a clinical isolate takes more time. Faster and simpler methods of diagnosis is of great advantage. That is why molecular biology technique is the first and foremost choice .
MOLECULAR BIOLOGY TECHNIQUES USED IN ZOONOTIC DISEASE
MOLECULAR BIOLOGY TECHNIQUES USED IN ZOONOTIC DISEASE
Nataraju S M
BLOTTING TECHNIQUES..........
Blotting techniques (manish)
Blotting techniques (manish)
manish chovatiya
Presentation Monica Stoian
Presentation Monica Stoian
Mihaiela Fazacas
It is most important ppt of northern blotting to educate the basic learner of Biotechnology
Northern blot
Northern blot
Aashish Patel
Fluorescent in situ hybridization (FISH) is a cytogenetic technique that uses fluorescent probes to investigate the presence of small, submicroscopic chromosomal changes that are beyond the resolution of karyotype analysis. This PowerPoint presentation explain the concept,process and application of Fluorescence insitu hybridization.
Fluorescence in situ hybridization
Fluorescence in situ hybridization
TukaramBorkar
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH) by Creative Diagnostics, learn more http://www.creative-diagnostics.com/in-situ-hybridization-and-fluorescence-in-situ-hybridization.htm
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH)
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH)
Creative-Diagnostics
Define karyotype and FISH Describe the procedure of karyotyping and FISH Explain chromosomal abnormalities through karyotyping and FISH Describe the principles of FISH Presented by- Dr. Subarna Das Resident, MS Anatomy Phase-A, Year-1, Block-2 Guided by- Prof. Laila Anjuman Banu Chairman Department of Anatomy, BSMMU
Karyotype and FISH
Karyotype and FISH
Rayhan Shahrear
Blotting
Northern, southern and western blotting
Northern, southern and western blotting
Ravi Kant Agrawal
techniques in molecular biology and cell line culturing with animal handelling
Various techniques in molecular bology
Various techniques in molecular bology
Kartikey Singh
Southern northern and western blotting
Southern northern and western blotting
Sana Javed
Blotting technology by using plant materials.
Blotting and its type
Blotting and its type
MonishaB2
A concise not on each of three different blotting technique
Blotting (Southern, Northern and Eastern)
Blotting (Southern, Northern and Eastern)
Aman Ullah
short and sweet description about blotting techniques
Blotting techniques
Blotting techniques
Kashikant Yadav
blotting and their types
Blotting southern,northern, western techniques
Blotting southern,northern, western techniques
veeralxmi
blotting techniques used to identify DNA, RNA and Protein and its Post-modification changes. blotting techniques are of three basis types; southern blotting western blotting eastern blotting
Blotting techniques
Blotting techniques
KinzaHaroon1
Southern Blotting is a technique developed by E.M. SOUTHERN BLOTTING in 1975. It includes Principle, steps and applications of southern blotting.
Southern blotting
Southern blotting
HariniV39
CHROMOSOME PAINTING: A METHOD FOR CELL CHARACTERIZATION
Chromosome painting
Chromosome painting
Prince Allawadhi
Molecular technique presentation by Dev vrat shukla
Fluorescent in situ hybridization technique
Fluorescent in situ hybridization technique
Devvrat Shukla
most effective slide for the medical student ,though you will find most of the things in short time..
Blotting techniques
Blotting techniques
Kashikant Yadav
blotting techniques
Blotting techniques
Blotting techniques
Alex Chris
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MOLECULAR BIOLOGY TECHNIQUES USED IN ZOONOTIC DISEASE
MOLECULAR BIOLOGY TECHNIQUES USED IN ZOONOTIC DISEASE
Blotting techniques (manish)
Blotting techniques (manish)
Presentation Monica Stoian
Presentation Monica Stoian
Northern blot
Northern blot
Fluorescence in situ hybridization
Fluorescence in situ hybridization
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH)
In situ Hybridization (ISH) and Fluorescence in Situ Hybridization (FISH)
Karyotype and FISH
Karyotype and FISH
Northern, southern and western blotting
Northern, southern and western blotting
Various techniques in molecular bology
Various techniques in molecular bology
Southern northern and western blotting
Southern northern and western blotting
Blotting and its type
Blotting and its type
Blotting (Southern, Northern and Eastern)
Blotting (Southern, Northern and Eastern)
Blotting techniques
Blotting techniques
Blotting southern,northern, western techniques
Blotting southern,northern, western techniques
Blotting techniques
Blotting techniques
Southern blotting
Southern blotting
Chromosome painting
Chromosome painting
Fluorescent in situ hybridization technique
Fluorescent in situ hybridization technique
Blotting techniques
Blotting techniques
Blotting techniques
Blotting techniques
Destacado
in suite hybridization (objective, applications, protocol)
Insuite hybridization
Insuite hybridization
Noman Ch
molecular breeding
Fish
Fish
Shreya Lodh
Methods and techniques for chromosomal in situ hybridization and molecular cytogenetics. Fixations, chromosomes preparation, mostly using plant chromosomes, hybridiziation mixtures, stringency calculations and fluorescent microscopy.Trude Schwarzacher and Pat Heslop-Harrison
In situ hybridization methods and techniques course slides Pat Heslop-Harrison
In situ hybridization methods and techniques course slides Pat Heslop-Harrison
Pat (JS) Heslop-Harrison
about the FISH technique and application http://biotechliveinfo.blogspot.com/2017/02/chromosome-analysis-fluorescent-in-situ.html
Fish(flourescent in-situ hybridization)
Fish(flourescent in-situ hybridization)
naren
Update on HER 2 testing in Breast Cancer
Update on HER2 testing
Update on HER2 testing
Dr. Pathmanathan
Microbiology
Microbiology
Zahoor Ahmed
Presentation Shahbaz
Presentation Shahbaz
Zahoor Ahmed
Bloood Bank
Bloood Bank
Zahoor Ahmed
Malaria Parasite
Malaria Parasite
Zahoor Ahmed
A Cytogenetic and molecular bio technique.
Genomic in situ Hybridization
Genomic in situ Hybridization
Kuldeep Sharma
FISH - a process which vividly paints chromosomes or portions of chromosomes with fluorescent molecules By : glok Productions
Fluorescence in situ Hybridization FISH #glok92
Fluorescence in situ Hybridization FISH #glok92
glok Productions
Poland presentaion 2
Poland presentaion 2
Sulushash
What is it? The INFORM HER2 Dual ISH DNA Probe Cocktail is a laboratory test that uses DNA probes, with color-forming (chromogenic) dyes attached, to count how many copies of the HER2 gene, located on chromosome 17, are present in a tissue sample. This test, which uses a variation of fluorescence in situ hybridization (ISH), is based on color detection, rather than fluorescence, and is used with samples of tumor tissue removed from breast cancer patients. This test is used by a doctor (pathologist) trained to identify diseases by studying slices of cells and tissues placed on microscope slides. If the test result indicates that too many copies of the HER2 gene (gene amplification) are present in the cancer cell, then a breast cancer patient may be eligible for treatment with the anti-cancer drug Herceptin® (Trastuzumab).
Inform Her2 DUAL ISH
Inform Her2 DUAL ISH
Brownb14
Advanced Staining End user meeting in Helsingør
Copenhagen michael gandy
Copenhagen michael gandy
Leica-Microsystems
credit seminar,cvas bikaner
Marker assisted selection
Marker assisted selection
DrSunil Bhakar
Karyotypes - Various
Karyotypes - Various
meducationdotnet
Esta proteína tem um papel importante no crescimento e desenvolvimento de uma vasta categoria de células, designadas por células epiteliais.
HIPEREXPRESSÃO DE RECEPTORES HER2/neu E SUAS ESTRATÉGIAS TERAPÊUTICAS AO CÂNC...
HIPEREXPRESSÃO DE RECEPTORES HER2/neu E SUAS ESTRATÉGIAS TERAPÊUTICAS AO CÂNC...
jose vanderlei oliveira dos reis
Karyotype
Karyotype
SBG404
In Situ Polymerase Chain Reaction (In situ PCR) is a powerful method that detects minute quantities of rare or single-copy number nucleic acid sequences in frozen or paraffin-embedded cells or tissue sections for the localization of those sequences within the cells. The principle of this method involves tissue fixing (to preserve the cell morphology) and subsequent treatment with proteolytic digestion (to provide access for the PCR reagents to the target DNA). The target sequences are amplified by those reagents and then detected by standard immunocytochemical protocols. In situ PCR combines the sensitivity of PCR or RT-PCR amplification along with the ability to perform morphological analysis on the same sample, and thus it is an attractive tool in diagnostic applications. One of the most prominent applications is the detection of infectious disease agents including HIV-1, HBV, HPV, HHV-6, CMV, and EBV.
Introduction to In situ pcr
Introduction to In situ pcr
BioGenex
By: Alejandro Hernández Martínez Third Semester Student of Medicine
Karyotype: The Molecules that define You
Karyotype: The Molecules that define You
Alejandro Hernández
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Insuite hybridization
Insuite hybridization
Fish
Fish
In situ hybridization methods and techniques course slides Pat Heslop-Harrison
In situ hybridization methods and techniques course slides Pat Heslop-Harrison
Fish(flourescent in-situ hybridization)
Fish(flourescent in-situ hybridization)
Update on HER2 testing
Update on HER2 testing
Microbiology
Microbiology
Presentation Shahbaz
Presentation Shahbaz
Bloood Bank
Bloood Bank
Malaria Parasite
Malaria Parasite
Genomic in situ Hybridization
Genomic in situ Hybridization
Fluorescence in situ Hybridization FISH #glok92
Fluorescence in situ Hybridization FISH #glok92
Poland presentaion 2
Poland presentaion 2
Inform Her2 DUAL ISH
Inform Her2 DUAL ISH
Copenhagen michael gandy
Copenhagen michael gandy
Marker assisted selection
Marker assisted selection
Karyotypes - Various
Karyotypes - Various
HIPEREXPRESSÃO DE RECEPTORES HER2/neu E SUAS ESTRATÉGIAS TERAPÊUTICAS AO CÂNC...
HIPEREXPRESSÃO DE RECEPTORES HER2/neu E SUAS ESTRATÉGIAS TERAPÊUTICAS AO CÂNC...
Karyotype
Karyotype
Introduction to In situ pcr
Introduction to In situ pcr
Karyotype: The Molecules that define You
Karyotype: The Molecules that define You
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BESIP Poster Panos FINAL
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Joseph Panos
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EVALUATE EXPRESSION OF EGFR PROTEIN BY IMMUNOHISTOCHEMISTRY STAINING TECHNIQUE AND MUTATION CHECK BY PCR METHOD
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Modern Cell Biology Techniques for Medical Research- Poster summary of 2010 summer internship at KU Cancer Center
10102010 Modern Cell Biology Techniques Fv[1]
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vponsamuel
There are so many techniques used in cytogenetics.
Different techniques used in cytogenetics
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Amit Jana
Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. Mammalian cell culture technology has become a major field in modern biotechnology; mammalian cell culture refers to the cells of a mammalian, isolated from specific tissues (i.e. skin, liver, glands, etc.) and further cultivated and reproduced in an artificial medium. Cell culture technology is currently playing a major role in toxicity testing, cancer research, virology, genetic engineering, and gene therapy. OBJECTIVE: To observe the transfection of CHO and HEK cells with GFP To observe the recombinant GFP using Western Blotting To purify the transfected HEK and CHO cells using AKTA Pure Purification
Mammalian project poster
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Nala Isaac (Nahla Ishaque)
Dr Dinah Parums. The Role of The Pathologist in Target Identification and Validation in Targeted Therapy and Personalized Medicine.
Dr Dinah Parums. The Role of The Pathologist in Target Identification and Val...
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Histotechniques aptitude questions
Histotechniques aptitude questions.pdf
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Vamsi kumar
131116 paper study 준섭
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virus isolation at by molicular technique
Virus Isolation II.pdf
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nezifzenu2023
Cell thawing,plating, trypsinizing, quantitation and cryofreezing
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Making Chromosome Slides
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marongen
131116 paper study 준섭
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The Survivin protein also known as BIRC5 (baculoviral inhibitor of apoptosis repeat-containing 5) is a member of the inhibitor of apoptosis family. It is encoded by the BIRC5 gene in humans. It is a multitasking protein through both promoting cell proliferation and preventing cell death. It is thought to regulate apoptosis through inhibiting caspase activation. The Survivin monoclonal antibody detects endogenous Survivin protein.
Antibody Customer Review for Anti-Survivin Monoclonal Antibody (STJ97436)
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St John's Laboratory Ltd
study of EGFR protein expression and mutation
study of EGFR protein expression and mutation
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2014 Scope poster
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CRISPR-Cas9 gene editing of human hematopoietic stem and progenitor cells (HSPCs) holds great promise for disease treatment by cellular therapy and other methodologies. However, the variables that can affect treatment of relevant diseases in the real world are still being elucidated. A critical part of this involves implementing an optimized gene editing and cell culture methodology. In this webinar, Dr. Marta Walasek and Ms. Danielle Nguyen Truong describe tips and tricks to lock down effective genome editing conditions for CD34+ cells, methods to evaluate genome editing efficiency, as well as optimal pre- and post-editing culture conditions to maintain HSPC function and long-term editing effects. The speakers outline the differences between primary CD34+ cell sources and the advantages of using StemSpan™ serum-free cell culture media for the maintenance of CD34 expression and expansion of primitive HSPC subsets during genome editing. Key considerations for designing CRISPR-Cas9 gene editing protocols are also reviewed by presenting a case study that demonstrates efficient genetic knockout of CD45 and β2 Microglobulin (B2M) in CD34+ HSPCs using the ArciTect™ CRISPR-Cas9 system. Key Topics Include: - Explore differences between sources of primary CD34+ cells - Identify optimized electroporation conditions for CD34+ cells from human cord blood - Define culture conditions for increased transfection efficiency of CD34+ cells - Assess cell viability and function post-transfection using flow cytometry and the CFU assay
Optimizing CD34+ Cell Genome Editing for Efficiency and HSPC Maintenance
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InsideScientific
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Platelet Transfusion Afshan
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A S S I N G M E N T
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