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ReaPan B27

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ReaPan B27 A Reagent for HLA-B27 Typing September 2008
What is HLA-B27 ? ,[object Object],[object Object],10% Anterior Uveitis 50% Inflammatory Bowel Disease 50-80% Reiter’s Syndrome 80% Rheumatoid Arthritis >90% Ankylosing Spondylitis Relative association with HLA-B27 antigen Condition
HLA-B27 typing ,[object Object],Diagnostic Methods in HLA-B27 typing PCR Platform Flow cytometry Platform ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Limitations of PCR based testing Advantages of PCR based testing Advantages of Flow Cytometry based testing Limitations of Existing Flow Cytometry based testing
Relative reagent performance for HLA-B27 typing ,[object Object],[object Object],Accuracy of Test Ease of Work Flow Assay Time Cost of Assay Parameter Performance Rating PCR Flow Cytometry
HLA-B27 typing in the Indian context Ankylosing Spondylitis (AS) is associated with HLA-B27 expression(>90%) Many cases suspected of AS are not referred for HLA-B27 typing High cost of Diagnosis BUT… WHY? High cost of HLA-B27 typing inhibits widespread prescription of the test
Designing a reagent for B27 typing – an India centric approach ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],ReaMetrix Solution: A Flow-Cytometry based test that offers accuracy closest to that of PCR
Rational design of B27 Reagent – The ReaMetrix approach ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Reagent to meet the desired performance requirements – ReaPan B27
ReaPan B27 assay protocol ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Dried Reagent Tube Add 25µl of Sample Vortex & Incubate for 30mins Add 300µl of ReaLyse Solution & Incubate for 15mins Analyze by Flow Cytometry
 Beads  Beads CD3+   Beads CD3+ HLA-B27+ Acquisition strategy ,[object Object],[object Object],[object Object],[object Object],[object Object]
Design comparison of flow based reagents ReaPan B27 has been designed for higher specificity and sensitivity ,[object Object],[object Object],[object Object],[object Object],[object Object],ReaPan B27 ,[object Object],[object Object],Discrimination of Positive and Negative Populations ,[object Object],[object Object],Selection of Fluorophores ,[object Object],[object Object],Coulter’s HLA-B27 Reagent ,[object Object],[object Object],Selection of Population BD’s HLA-B27 Reagent Parameter
Relative reagent performance for HLA-B27 typing ,[object Object],[object Object],[object Object],[object Object],Accuracy of Test Ease of Work Flow Assay Time Cost of Assay Parameter Performance Rating PCR Flow Cytometry ReaPan B27
Sensitivity of ReaPan B27 Reagent = 96% Specificity of ReaPan B27 Reagent = 95% Accuracy of ReaPan B27 Reagent = 96% Comparison of assay performance with other reagents 71 43 28 TOTAL 42 41 1 Negative 29 2 27 Positive ReaPan B27 Negative Positive TOTAL Bd’s HLA-B27 Reagent Decision Matrix
Reference articles - Ankylosing Spondylitis ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],Reference articles - Ankylosing Spondylitis
[object Object],[object Object],[object Object],[object Object],Reference articles - Ankylosing Spondylitis
Reference articles - Uveitis ,[object Object],[object Object],[object Object],[object Object]
Manufactured by ReaMetrix India Pvt. Ltd. 50-B, II Phase, Peenya Industrial Area Peenya, Bangalore 560058, India Ph: +91-80-28378693/5, Fax: +91-80-41172451 E-mail: info@reametrix.com www.reametrix.com Further details:

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ReaPan B27

  • 1. ReaPan B27 A Reagent for HLA-B27 Typing September 2008
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  • 5. HLA-B27 typing in the Indian context Ankylosing Spondylitis (AS) is associated with HLA-B27 expression(>90%) Many cases suspected of AS are not referred for HLA-B27 typing High cost of Diagnosis BUT… WHY? High cost of HLA-B27 typing inhibits widespread prescription of the test
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  • 12. Sensitivity of ReaPan B27 Reagent = 96% Specificity of ReaPan B27 Reagent = 95% Accuracy of ReaPan B27 Reagent = 96% Comparison of assay performance with other reagents 71 43 28 TOTAL 42 41 1 Negative 29 2 27 Positive ReaPan B27 Negative Positive TOTAL Bd’s HLA-B27 Reagent Decision Matrix
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  • 17. Manufactured by ReaMetrix India Pvt. Ltd. 50-B, II Phase, Peenya Industrial Area Peenya, Bangalore 560058, India Ph: +91-80-28378693/5, Fax: +91-80-41172451 E-mail: info@reametrix.com www.reametrix.com Further details: