seminario biologia molecular, tercer semestre, universidad pontificia bolivariana.

1. Alejandro Cañaveral Taborda
third semester
pontifical bolivarian university

2. INTRODUCTION
Bladder cancer is an alteration that occurs when there is a mutation of the
urothelial cells that line the bladder, it can expand easily

3. are small non-coding RNA molecules that
regulate gene expression at the post-
transcriptional level of bladder cancer
What are microRNA497-5p?

4. We aim to develop a potential
competing endogenous RNA (ceRNA)
network related with CEP55 in BC.
GENERAL OBJECTIVE

5. METHODS
RNA extraction and quantitative
real‑time PCR
Flow cytometry analysis of
apoptosis
RNA was extracted
from patient samples
That rna was passed
to a dna copy by
reverse transcription
Rt pcr was used for
the amplification of
the dna copy
One of the most
popular and
versatile
applications for
studying apoptosis

6. Luciferase reporter assay Western blot
A protein is used
that, when it detects
transcription
activity, will release
the luciferase
enzyme that will
allow it to identify
miRNAs.
Monitor
antibodies that
react with the
protein of interest

7. RESULTS

9. DISCUSSION

10. CONCLUSIONS
Important for its
contribution to the
eradication of
diseases
scientific and
research
advances