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Crispr cas9 technology
Crispr cas9 technology
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Crispr

  1. 1. PRESENTED BY: AHMED MADNI REGISTRATION: SP14-BTY-011
  2. 2.  CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)  Essential in adaptive immunity in selected bacteria and archaea, enabling the organisms to respond to and eliminate invading genetic material  These repeats were initially discovered in the 1980s in E. coli 7 December 2015CRISPR/CAS9 2
  3. 3.  This technology is used to edit any genome sequence  CRISPR has molecular scissor that can selectable disable or change Gene sequence  Used in Gene therapy treatment to cancer and Inherited disorder 7 December 2015CRISPR/CAS9 3
  4. 4. 7 December 2015CRISPR/CAS9 4
  5. 5. 7 December 2015CRISPR/CAS9 5  Guide RNA  Cas9 Endonuclease
  6. 6. 7 December 2015CRISPR/CAS9 6
  7. 7. Non-homologous end joining  Ligation method Random mutation Insertion/deletion Gene knockout Homologous direct repair  Repair Templete  Highly accurate method introduce specific nucleotide Gene knockout Gene tagging Specific mutation Gene knockin Promotor study 7 December 2015CRISPR/CAS9 7
  8. 8. 7 December 2015CRISPR/CAS9 8
  9. 9.  Creating a Double Strand Break, while minimizing off- target effect 7 December 2015CRISPR/CAS9 9
  10. 10. • Transcriptional activator • Transcriptional repressor • DNA labeling 7 December 2015CRISPR/CAS9 10
  11. 11. 7 December 2015CRISPR/CAS9 11
  12. 12.  Simple  Effective  Use Genetic therapeutics Medicine Cell and molecular research 7 December 2015CRISPR/CAS9 12
  13. 13. 7 December 2015CRISPR/CAS9 13

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