CRISPR TECHNOLOGY.. Its mechanism and Benefits..

1. PRESENTED BY: AHMED MADNI
REGISTRATION: SP14-BTY-011

2.  CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)
 Essential in adaptive immunity in selected bacteria and archaea,
enabling the organisms to respond to and eliminate invading genetic
material
 These repeats were initially discovered in the 1980s in E. coli
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3.  This technology is used to edit any genome sequence
 CRISPR has molecular scissor that can selectable disable or
change Gene sequence
 Used in Gene therapy treatment to cancer and Inherited disorder
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 Guide RNA
 Cas9 Endonuclease

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7. Non-homologous end joining
 Ligation method
Random mutation
Insertion/deletion
Gene knockout
Homologous direct repair
 Repair Templete
 Highly accurate method introduce
specific nucleotide
Gene knockout
Gene tagging
Specific mutation
Gene knockin
Promotor study
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9.  Creating a
Double Strand
Break, while
minimizing off-
target effect
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10. • Transcriptional activator
• Transcriptional
repressor
• DNA labeling
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12.  Simple
 Effective
 Use
Genetic therapeutics
Medicine
Cell and molecular research
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