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Lab 1 – The Scientific Method 
and Termite Trails 
#NSB2014 1
Welcome to NSB 
(Natural Science Biology) 
• Why is Biology important? 
– You encounter living things, and forces that act on living things 
every day 
– Stem cells, cloning, DNA, genetic testing, vaccines, evolution, 
bioethics, animal and plant diversity, mutations, antibiotic 
resistance, viruses, diseases, global warming, energy, fossil 
fuels, genetically engineered foods. 
– You want to be informed, so you can make smart choices for 
yourself, for your family, for your community, and for the world. 
#NSB2014 2
Why do I need to take a lab? 
• We want you to be able to “think like a scientist” 
• Labs provide a hands-on, active approach to 
learning. 
• Labs demonstrate The Scientific Method. 
• Labs confirm that simple research can affect the 
way you approach problems. 
• Lab fulfills the “science course with a lab” 
requirement for general education 
#NSB2014 3
Natural Science Syllabus 
Introduction 
• Grading 
• Quizzes 
• Attendance 
• Participation 
• Paper/Presentation 
• Code of Conduct 
• Electronic Communications 
#NSB2014 4
Lab Manual 
• Must be purchased for lab. 
Available in Bookstore. 
• Needed every week to turn 
in your questions. This is 
how you get your 
participation points. 
• You must have your lab 
manual with you each lab, 
to have instructions for the 
lab. If you do not have your 
own set of instructions, you 
cannot participate in lab. 
#NSB2014 5
In the lab - Lab Equipment 
• Always check to make sure you have all the 
needed equipment before you begin your lab. 
• Lab equipment must always be stored properly 
and cleaned to avoid contamination (Becoming 
nonsterile by contact with any nonsterile 
material). Never put away equipment that is 
dirty. 
#NSB2014 6
In the lab - Safety Procedures 
1. Never conduct any 
laboratory experiments 
until proper instructions 
have been given and a 
instructor is present. 
2. If something goes 
wrong while performing 
a laboratory 
experiment, 
immediately get the 
attention of the 
instructor. 
3. Always read the 
instructions and safety 
precautions before 
beginning an 
experiment. 
#NSB2014 7
In the lab - Designing the 
Experiment 
• Each of the lab has been designed to give you a 
“big picture” view of Biology. 
• Each lab has the same format – a short lecture, 
background information, materials, the 
procedure, collecting data, and questions 
designed to assess your understanding. 
• Each lab has a quiz over vocabulary and concepts 
• Each lab is conducted using “The Scientific 
Method” 
#NSB2014 8
The Scientific Method starts, stops, 
moves forward, moves backward, and 
rarely just follows the circle. 
#NSB2014 9
Much of what we do in science does 
not involve experiments 
• Much of science is making observations and 
descriptions. 
– Different sizes 
– Different colors 
– Different structures 
– Different functions 
– Then, we make predictions about why things 
might be different 
#NSB2014 10
Forming a Hypothesis 
• Forming a hypothesis first involves 
observation. 
• Then, ask a question about something you 
observe. Your hypothesis will be a possible 
answer to that question. 
• Remember a hypothesis does not have to be 
correct; it simply has to be testable. 
• In fact, often hypotheses are wrong. However, 
even a wrong hypothesis is useful because it 
helps you rule out ideas. 
#NSB2014 11
Examples of Hypotheses We Can Test 
• More students get sick during the final week of testing that at 
other times. 
• One solar panel can replace one C battery. 
• Disinfectants that contain bleach will kill 99% of E. coli bacteria 
on hard surfaces. 
• Worker satisfaction increases worker productivity. 
• Amount of sun exposure will increase the growth of a tomato 
plant. 
• Childhood obesity is tied to the amount of sugary drinks 
ingested daily. 
• A dog can be trained to alert a human if the telephone is ringing. 
#NSB2014 12
Testing a Hypothesis 
• You must 
design a 
controlled 
experiment to 
test a 
hypothesis. A 
controlled 
experiment 
tests only one 
factor at a time. 
• What would be 
included in the 
control, besides 
soil? 
#NSB2014 13
Dependent v. Independent Variable 
• Independent Variable – Changed 
– The one factor that you alter between experimental 
groups 
• Dependent Variable– Measured 
– Your result (data), (depends on what is changed) 
#NSB2014 14
Data Collection Techniques 
• All of the information collected during a scientific 
investigation is known as data. Data can be collected 
through the following techniques: 
• Quantitative measurement – numerical (such 
as weight, volume, or how many legs) 
• Qualitative observation – descriptive (such as 
colors, size, shape, feeling) 
#NSB2014 15
Analyzing Data 
• Data can be analyzed by making inferences 
from and recognizing patterns in the data. You 
might use the following methods to analyze 
your data: 
– statistical analysis 
– creating graphs 
– organizing data into tables and charts 
#NSB2014 16
Data Collection and Sample Size 
• The larger the 
amount of data 
collected, 
• The larger the 
sample size, 
• The more times the 
experiment is 
repeated, 
• The more valid the 
results. 
#NSB2014 17
Collect and Interpret Data 
• When you collect data you need to be 
concerned about precision and accuracy. 
• Precision in Data Collection 
– Precision refers to how consistent and 
reproducible a measurement is. A good rule of 
thumb is to take measurements two or three 
times (called replicates) in order to ensure 
precision 
• Accuracy in Data Collection 
– Accuracy refers to the correctness of a 
measurement. Data will be useless unless it is 
collected accurately. 
#NSB2014 18
Precision vs. Accuracy 
#NSB2014 19 
All the measurements are 
close together 
All the measurements are 
close to center
• Which piece of 
equipment would be 
best for accurately 
measuring 76 ml? 
#NSB2014 20
Do I have a theory, or do I have a 
hypothesis? 
• Scientists use the word “theory” differently than 
the general public. A theory in science is not a 
guess. 
• A scientific theory is a set of principles that 
explain and predict phenomena, after repeated 
observations and experiments. 
• Once a hypothesis is verified, it becomes a 
theory. 
• Examples of scientific theories – cell theory, 
evolutionary theory, big bang theory, theory of 
relativity 
#NSB2014 21
What’s YOUR hypothesis? 
• The termites used in this experiment are a 
group of social insects usually classified at the 
taxonomic rank of order Isoptera. As truly 
social animals, they are termed eusocial . 
• Eusocial animals display several characteristics 
– they have a queen, workers or laborers, 
defenders, colonies, castes – but why? 
#NSB2014 22
How to “do” the lab 
• Read the background information 
• Look at your materials and procedure – what 
are you going to do, and why? 
• Follow directions, and fill in your data sheet 
for Lab 1. 
• Make sure your full, real name, section, and 
TA are on the handout, and turn it in. 
• You aren’t finished until your lab station is 
clean and ready for the next class. 
#NSB2014 23

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Lab 1 – the scientific method and termite trails fall 2014

  • 1. Lab 1 – The Scientific Method and Termite Trails #NSB2014 1
  • 2. Welcome to NSB (Natural Science Biology) • Why is Biology important? – You encounter living things, and forces that act on living things every day – Stem cells, cloning, DNA, genetic testing, vaccines, evolution, bioethics, animal and plant diversity, mutations, antibiotic resistance, viruses, diseases, global warming, energy, fossil fuels, genetically engineered foods. – You want to be informed, so you can make smart choices for yourself, for your family, for your community, and for the world. #NSB2014 2
  • 3. Why do I need to take a lab? • We want you to be able to “think like a scientist” • Labs provide a hands-on, active approach to learning. • Labs demonstrate The Scientific Method. • Labs confirm that simple research can affect the way you approach problems. • Lab fulfills the “science course with a lab” requirement for general education #NSB2014 3
  • 4. Natural Science Syllabus Introduction • Grading • Quizzes • Attendance • Participation • Paper/Presentation • Code of Conduct • Electronic Communications #NSB2014 4
  • 5. Lab Manual • Must be purchased for lab. Available in Bookstore. • Needed every week to turn in your questions. This is how you get your participation points. • You must have your lab manual with you each lab, to have instructions for the lab. If you do not have your own set of instructions, you cannot participate in lab. #NSB2014 5
  • 6. In the lab - Lab Equipment • Always check to make sure you have all the needed equipment before you begin your lab. • Lab equipment must always be stored properly and cleaned to avoid contamination (Becoming nonsterile by contact with any nonsterile material). Never put away equipment that is dirty. #NSB2014 6
  • 7. In the lab - Safety Procedures 1. Never conduct any laboratory experiments until proper instructions have been given and a instructor is present. 2. If something goes wrong while performing a laboratory experiment, immediately get the attention of the instructor. 3. Always read the instructions and safety precautions before beginning an experiment. #NSB2014 7
  • 8. In the lab - Designing the Experiment • Each of the lab has been designed to give you a “big picture” view of Biology. • Each lab has the same format – a short lecture, background information, materials, the procedure, collecting data, and questions designed to assess your understanding. • Each lab has a quiz over vocabulary and concepts • Each lab is conducted using “The Scientific Method” #NSB2014 8
  • 9. The Scientific Method starts, stops, moves forward, moves backward, and rarely just follows the circle. #NSB2014 9
  • 10. Much of what we do in science does not involve experiments • Much of science is making observations and descriptions. – Different sizes – Different colors – Different structures – Different functions – Then, we make predictions about why things might be different #NSB2014 10
  • 11. Forming a Hypothesis • Forming a hypothesis first involves observation. • Then, ask a question about something you observe. Your hypothesis will be a possible answer to that question. • Remember a hypothesis does not have to be correct; it simply has to be testable. • In fact, often hypotheses are wrong. However, even a wrong hypothesis is useful because it helps you rule out ideas. #NSB2014 11
  • 12. Examples of Hypotheses We Can Test • More students get sick during the final week of testing that at other times. • One solar panel can replace one C battery. • Disinfectants that contain bleach will kill 99% of E. coli bacteria on hard surfaces. • Worker satisfaction increases worker productivity. • Amount of sun exposure will increase the growth of a tomato plant. • Childhood obesity is tied to the amount of sugary drinks ingested daily. • A dog can be trained to alert a human if the telephone is ringing. #NSB2014 12
  • 13. Testing a Hypothesis • You must design a controlled experiment to test a hypothesis. A controlled experiment tests only one factor at a time. • What would be included in the control, besides soil? #NSB2014 13
  • 14. Dependent v. Independent Variable • Independent Variable – Changed – The one factor that you alter between experimental groups • Dependent Variable– Measured – Your result (data), (depends on what is changed) #NSB2014 14
  • 15. Data Collection Techniques • All of the information collected during a scientific investigation is known as data. Data can be collected through the following techniques: • Quantitative measurement – numerical (such as weight, volume, or how many legs) • Qualitative observation – descriptive (such as colors, size, shape, feeling) #NSB2014 15
  • 16. Analyzing Data • Data can be analyzed by making inferences from and recognizing patterns in the data. You might use the following methods to analyze your data: – statistical analysis – creating graphs – organizing data into tables and charts #NSB2014 16
  • 17. Data Collection and Sample Size • The larger the amount of data collected, • The larger the sample size, • The more times the experiment is repeated, • The more valid the results. #NSB2014 17
  • 18. Collect and Interpret Data • When you collect data you need to be concerned about precision and accuracy. • Precision in Data Collection – Precision refers to how consistent and reproducible a measurement is. A good rule of thumb is to take measurements two or three times (called replicates) in order to ensure precision • Accuracy in Data Collection – Accuracy refers to the correctness of a measurement. Data will be useless unless it is collected accurately. #NSB2014 18
  • 19. Precision vs. Accuracy #NSB2014 19 All the measurements are close together All the measurements are close to center
  • 20. • Which piece of equipment would be best for accurately measuring 76 ml? #NSB2014 20
  • 21. Do I have a theory, or do I have a hypothesis? • Scientists use the word “theory” differently than the general public. A theory in science is not a guess. • A scientific theory is a set of principles that explain and predict phenomena, after repeated observations and experiments. • Once a hypothesis is verified, it becomes a theory. • Examples of scientific theories – cell theory, evolutionary theory, big bang theory, theory of relativity #NSB2014 21
  • 22. What’s YOUR hypothesis? • The termites used in this experiment are a group of social insects usually classified at the taxonomic rank of order Isoptera. As truly social animals, they are termed eusocial . • Eusocial animals display several characteristics – they have a queen, workers or laborers, defenders, colonies, castes – but why? #NSB2014 22
  • 23. How to “do” the lab • Read the background information • Look at your materials and procedure – what are you going to do, and why? • Follow directions, and fill in your data sheet for Lab 1. • Make sure your full, real name, section, and TA are on the handout, and turn it in. • You aren’t finished until your lab station is clean and ready for the next class. #NSB2014 23