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Brief Introduction of Fatty Acids

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If you want to know more, please visit: https://www.creative-proteomics.com/ Fatty acids refer to the carboxylic acid with a long aliphatic chain. They can be classified based on different factors, like the length and the number of double bonds. Fatty acids are important components of the human body and they have biological, structure and functional roles.

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Brief Introduction of Fatty Acids

  1. 1. Brief Introduction of Fatty Acids Presented by Creative Proteomics Types, Roles, and Analysis
  2. 2. Fatty acids (FAs) are hydrocarbon chains of varying lengths and degrees of unsaturation (the presence of double bonds), with a carboxyl group at one end and a methyl group at the other. The most abundant fatty acids have even numbers of carbons, with a straight chain, although exceptions do exist. Introduction
  3. 3. Types of Fatty Acides fatty acids with aliphatic tails of 6 to 12 carbons, which can form medium-chain triglycerides (MCT) Very long chain fatty acids (VLCFAs) fatty acids with a chain-length of 22 or more carbons Length Medium-chain fatty acids (MCFA) fatty acids with two to six carbon atoms Long-chain fatty acids (LCFA) fatty acids with aliphatic tails of 13 to 21 carbons Short-chain fatty acids (SCFA)
  4. 4. Types of Fatty Acides 01 02 03 Saturated FAs (SFAs) Monounsaturated FAs (MUFAs) Polyunsaturated FAs (PUFAs) The number of double bonds
  5. 5. Fatty acids are important components of the human body and they have biological, structural and functional roles.
  6. 6. Roles of Fatty Acides The major source of energy • Fatty acids are stored as triacylglycerols (TAGs) in adipose tissue and to some extent, in skeletal and cardiac muscle. • The fatty acids released by TAG hydrolysis are used primarily for the production of energy in the fatty acid β-oxidation pathway in mitochondria and peroxisomes. Rustan A C, Drevon C A. Fatty acids: structures and properties. e LS, 2001
  7. 7. Roles of Fatty Acides Membrane components Fatty acids act as main constituents of cellular membranes. The fatty acid composition of the lipid bilayer influences the fluidity of the membrane, which in turn can modify the activities and interactions between integral proteins. When ingesting very long chain ω-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are highly incorporated into membrane phospholipids, which may change the physical characteristics of the membranes. Rustan A C, Drevon C A. Fatty acids: structures and properties. e LS, 2001
  8. 8. Roles of Fatty Acides Signal Transduction Fatty acids are involved in a wide range of biological signaling pathways. Fatty acids play important roles in the formation of eicosanoids that are a group of signaling molecules involved in the immune response. The main precursor for eicosanoid synthesis is often arachidonic acid. Rustan A C, Drevon C A. Fatty acids: structures and properties. e LS, 2001
  9. 9. Roles of Fatty Acides Acylation of Protein The acylation of proteins plays important roles in anchoring certain proteins in membranes, folding proteins, and the function of multiple proteins. Some proteins are acylated with stearic acids, palmitic acids or myristic acids. Both saturated fatty acids and polyunsaturated fatty acids acylate proteins. Rustan A C, Drevon C A. Fatty acids: structures and properties. e LS, 2001
  10. 10. Roles of Fatty Acides Gene Expression Fatty acids or their derivatives (acyl-CoA or eicosanoids) may alter gene transcription by interacting with nuclear receptor proteins that bind to certain regulatory regions of DNA. The combined fatty acid-receptor complex may function as a transcription factor. Fatty acids can modify transcription directly via the family of transcription factors known as peroxisome proliferator-activated receptors (PPARs). Rustan A C, Drevon C A. Fatty acids: structures and properties. e LS, 2001
  11. 11. Fatty Acids Analysis Some methods have been used for fatty acids analysis, including gas chromatography (GC), high- performance liquid chromatography (HPLC), near-infrared spectroscopy (NIRS), and NMR spectroscopy. Each method has its own advantages and limits. Wu Z, et al. Comparison of critical methods developed for fatty acid analysis: a review. Journal of separation science, 2017, 40(1): 288-298.
  12. 12. Fatty Acids Analysis • GC appears to be the most commonly used method of fatty acids analysis. GC methods with high-quality capillary columns are used for sensitive and reproducible fatty acids analysis. • Fatty acids exhibit low volatility and strong polarity. They easily undergo polymerization, deacidification, cracking, and other adverse reactions at high temperatures. As such, fatty acids are indirectly analyzed by GC. • Before GC analysis, fatty acids should be derived to increase their volatility and decrease their polarity. Converting fatty acids into fatty acid methyl esters (FAMEs) is a classical and commonly used method for deriving fatty acids. • GC coupled with a flame ionization detector (FID) is traditionally used to analyze fatty acids. In addition, GC is frequently combined with mass spectrometry (MS) for highly selective and sensitive quantitative analysis.
  13. 13. Fatty Acids Analysis Services At Creative Proteomics, we have developed a professional platform for the analysis of fatty acids by GC. The fatty acids we can provide include: • Short Chain Fatty Acids Analysis Service • Medium-chain and Long-chain Fatty Acids Analysis Service • Very Long Chain Fatty Acids Analysis Service • Straight Chain Fatty Acids Analysis Service • Branched Chain Fatty Acids Analysis Service • Total Fatty Acids Analysis Service • Free Fatty Acids (FFAs) Analysis Service
  14. 14. Thanks Presented by Creative Proteomics Please contact us for more information Website: www.creative-proteomics.com Email: info@creative-proteomics.com

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  • Hello, welcome to watch the creative proteomics . Today, we are going to talk about Short Chain Fatty Acids Analysis

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