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The Application and Methods for Peptidomics

  1. 1. | Creative Proteomics Presentation | The Application and Methods for Peptidomics
  2. 2. The peptidomics means the comprehensive visualization and analysis of small polypeptides, thus covering the mass range between proteomics and metabonomics. During the completion of the human genome, a wide variety of additional “omics” technologies arose, such as transcriptomics. There is no clear definition to distinguish between peptides and proteins. The most common description is that peptides range from two amino acids (dipeptides) to small proteins with a molecular mass of 20 kDa. There are large numbers of peptides in human body fluids and have many possible functionalities.
  3. 3. Peptidomics in Biomarkers The data from a biomarker can be applied to diagnostic implications, molecular and cellular events of the pathology, and new therapeutic approaches. C-peptide of proinsulin in diabetes natriuretic peptides in heart diseases amyloid peptides correlated with Alzheimer's • Qualitative analysis: show which peptides are present in a sample • Quantitative analysis: discover the concentration
  4. 4. Peptidomics in Drug Targets Small molecules Antibodies Peptides Proteins • With the developments in several technologies in the areas of peptide synthesis, screening, stability, and modifications, peptides are now recognized as lead molecules for therapeutics. • Features :Important peptide properties are their chemical and biological diversity, high specificity, high affinity and potency, and unique 3D characteristics. They also show no accumulation in organs, usually display a low toxicity at therapeutic doses, and are less immunogenic than antibodies. • Drawbacks. They are low stability in body fluids and the difficulty in transporting them to target organs.
  5. 5. Peptidomics in Drug Targets There are more than 67 peptide drugs are currently on the market. Adrenocorticotropic hormone Amyloid Beta- peptide Calcitonin Desmopressin Incretin mimetics Insulin Leuprolide acetate Oxytocin Pramlintide acetate.
  6. 6. Technologies of Peptidomics Peptides analysis needs preparation and analysis methods that are substantially different from those typically used for proteins. In peptidomics studies, it’s important to control proteolysis, not only to avoid peptide degradation but also mainly to prevent contaminating the peptide sample with proteolytic fragments of larger proteins from the same source.
  7. 7. Technologies of Peptidomics The peptides have some characteristics, including the peptide size and charge state, which complicate the analysis of endogenous peptides. • The endogenous peptides range in size from 2 to over 100 amino acids. • In addition, the charge state of the endogenous peptides is not nearly as uniform as for tryptic peptides. • And because of the blocked N-terminus and the absence of lysine, some endogenous peptides have no positive charge. However, the goal of most peptidomics studies is to identify all of the abundant peptides, including some peptides with positive charges or large sizes. Because peptides vary in multiple aspects, multiple peptide purification methods can be used sequentially on the same sample. But this strategy has some drawbacks, like limiting the reproducibility of the method and increasing sample preparation time.
  8. 8. Technologies of Peptidomics With the development of electrospray ionization (ESI) and matrix-assisted laser desdorption/ionization (MALDI), mass spectrometry has become an ideal tool for peptide analysis. These soft ionization methods can ionize intact peptide molecules and acceleration into a vacuum without substantial fragmentation. ESI MALDI
  9. 9. Technologies of Peptidomics • Various mass analyzers have applied to peptidomics, including orbitraps, quadrupole time-of-flight (Q- TOF), and micro-TOF. In addition, tandem mass spectrometry is required for additional information for sequencing. • There are some commonly used fragmentation techniques for peptidomics, including collision- induced dissociation (CID), electron transfer dissociation (ETD) and electron-capture dissociation (ECD).
  10. 10. Our Services Equipped with high resolution mass spectrometer, the bioanalysts and technicians in Creative Proteomics can offer professional assistance in your peptidomics research, including characterization of peptide biomarkers and comprehensive peptidomics services.
  11. 11. T h a n k s Please contact us for more information Web: Email: www.creative-proteomics.com info@creative-proteomics.com

Notas

  • Hello, welcome to watch Creative Proteomics’ Video. Today, we are going to briefly introduce The application and methods for peptidomics.
  • Peptidomics can be used for biomarker discovery. The data from a biomarker can be applied to diagnostic implications, molecular and cellular events of the pathology, and new therapeutic approaches. Many peptidomics studies are searching for molecular analytes which correlate with disease states, including C-peptide of proinsulin in diabetes, natriuretic peptides in heart diseases or amyloid peptides correlated with Alzheimer's. Peptide biomarkers have to fulfill two tasks, including qualitative analysis and quantitative analysis, which will allow for the selection of those peptides that are valuable for the desired drug development process. Qualitative analysis means to show which peptides are present in a sample while quantitative analysis means to discover the concentration.
  • There are more than 67 peptide drugs are currently on the market. Some examples of the therapeutic potential of endogenous peptides include Adrenocorticotropic hormone (ACTH), b-Amyloid peptides (A β), Calcitonin, Desmopressin, Incretin mimetics, Insulin, Leuprolide acetate, Oxytocin, and Pramlintide acetate.
  • The study of endogenous peptides is often compromised by protein fragments produced post mortem during conventional sample handling. Peptides analysis needs preparation and analysis methods that are substantially different from those typically used for proteins.
    In peptidomics studies, it’s important to control proteolysis, not only to avoid peptide degradation but also mainly to prevent contaminating the peptide sample with proteolytic fragments of larger proteins from the same source.
  • The peptides have some characteristics, including the peptide size and charge state, which complicate the analysis of endogenous peptides. The endogenous peptides range in size from 2 to over 100 amino acids. In addition, the charge state of the endogenous peptides is not nearly as uniform as for tryptic peptides. And because of the blocked N-terminus and the absence of lysine, some endogenous peptides have no positive charge. However, the goal of most peptidomics studies is to identify all of the abundant peptides, including some peptides with positive charges or large sizes. Because peptides vary in multiple aspects, multiple peptide purification methods can be used sequentially on the same sample. But this strategy has some drawbacks, like limiting the reproducibility of the method and increasing sample preparation time.
  • Thanks for watching our video. At creative proteomics, we provide the most reliable peptidomics services. If you have any questions or specific requirements. Please feel free to contact us. We are very glad to cooperate with you.
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