This document summarizes several Gram positive and Gram negative bacilli of medical importance, including Corynebacterium diphtheriae, Bacillus anthracis, Salmonella, Shigella, E. coli, Klebsiella, Proteus, and Yersinia pestis. It describes their morphology, pathogenicity, diseases caused, specimen collection and testing methods, and appearance on common culture media such as blood agar, MacConkey agar, and Hektoen agar. Key identification characteristics include diphtheria's "Chinese letters" morphology, anthrax's spore formation, Salmonella's black-centered colonies on IS media, and Proteus's swarming growth pattern.
3. Genus Corynebacterium
Species Corynebacterium diphtheriae
- high pathogenicity
- Clinical significance: diphtheria = disease produced by
the diphtheric toxine
- → symptoms at the entry gate: sore throat, adherent
membrane (pseudomembrane) on tonsils, pharynx,
nasal cavity
- → general toxic symptoms: fever (hematogenic difusion
of diphtheric toxin)
5. Species Corynebacterium diphtheriae
Laboratory diagnosis
• Colection of specimens: throat / nasal / wound swab
• Microscopy:
– Gram positive bacilli, aspect of ”Chinese letters” / capital letters
– low value (C.diphtheriae – similar to other comensal
corynebacteria in the throat)
• Cultivation:
– Blood agar
– Selective media with tellurite (Tinsdale, Gundel-Tietz)
– Highly selective Loffler medium
10. Genus Bacillus
Species Bacillus anthracis
• Large, Gram positive rods (10
μM),
• straight cut ends,
• aerobic,
• disposed in chains
• Spore forming
11. Survival outside host
(continued)
Spores: reproductive structures adapted
for longtime survival in unfavourable
conditions
(etymology: ancient Greek spora = seed)
Bacterial spores - outer layer of keratin resistant to chemicals, staining and
heat → bacterium able to stay dormant for years, protected from
temperature differences, absence of air, water and nutrients
Spore forming bacteria:
• Clostridium spp (e.g. Clostridium difficile, Clostridium tetani);
• Bacillus spp (B. anthracis).
12. Bacillus anthracis (continued)
• High pathogenicity
• Disease = zoonosis (infection of animals AND humans)
• Clinical forms:
– Cutaneous anthrax – spores enter the body via skin lesions
– Pulmonary anthrax – inhalatory infection
– Digestive anthrax – ingestion of infected undercooked meat
– + biological weapon (inhalatory infection) – agent of bioterrorism
13. Left: anthrax skin lesion
Right: ”anthrax attack” letter (1 week after 9/11)
15. Family Enterobacteriaceae
• Gram negative rods, non-spore forming, non-fastidious
• Wide spread in the environment (plants, soil, water,
human and animal intestines, on mucous membranes,
etc)
• Clinical significance:
– intestinal and extraintestinal infections
• Collection of specimens in intestinal infections:
– stool (faeces)
• Collection of specimens in extraintestinal infections:
– Urine, ear secretion, wound exudate, CSF, etc
16. Collection of stool (faeces)
• Disposable stool collection containers (simple / with
transportation medium Carry Blair: non-nutritive medium
which prevents overgrowth of Enterobacteriaceae but
preserves viable enteric pathogens (Salmonella,
Shigella, Vibrio, etc)
17. Collection of urine
When?:
- in the morning (first miction)
How?:
- clean uro-genital area
- eliminate first flow
- collect middle flow in
sterile container
Send to lab immediately or store
at 2-8°C
18. Collection of cerebrospinal fluid (CSF)
Lumbar punction (spinal tap)
• patient lies on the side, knees pulled up toward
chest, chin tucked downward
• back cleaned and disinfected (iodine) + health
care provider injects local anesthetic into lower
spine
• spinal needle inserted into lower back area
• needle properly positioned, CSF pressure
measured and sample collected in sterile tube
• needle removed, area cleaned, bandage placed
over puncture site
19. Genus Yersinia
Species:
• Yersinia enterocolitica:
– colonizes the intestines; only some strains are pathogenic – may
cause diarrhoea + apendicitis-like symptoms
• Yersinia pestis (causative agent of plague)
– reservoir of germs: rodents (rats)
– interpersonal transmission (human to human)
– Routes of infection:
• Vectors: Flea bites →skin lesions (inflammation, necrosis, purulent
secretion) + swollen lymph nodes (buboes) = bubonic plague
→sepsis
• Airborne: Inhalation →pneumonia = pulmonary plague
34. Genus Proteus
• Facultatively pathogenic
• Colonizes the intestines
• May cause infections in case of immunesuppression e.g.
urinary infections, synusitis, otitis, meningitis + hospital
acquired infections
• Cultivation: characteristic ”invasion” of the culture
medium – the swarming phenomenon – favours biofilm
formation → catheter infections