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Wang_David_Reseach_Paper_Abstract

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Wang_David_Reseach_Paper_Abstract

  1. 1. Wang Distinguishing ​C. elegans​ chromosomes with amplicon size polymorphisms 1 Distinguishing ​C. elegans​ chromosomes with amplicon size polymorphisms David Wang​1​ , Hannah Lyman​2​ , Elizabeth Vargas​2​ , Frank McNally​2​ , Ian Korf​1,2 1​ UC Davis Genome Center, University of California, Davis, California 95616, USA; ​2​ Department of Molecular and Cellular Biology, University of California, Davis, California 95616, USA Abstract Researchers investigating chromosomal loss in aneuploid ​C. elegans​ currently have no cost-effective method of distinguishing homologous chromosomes from different strains. Even though genome sequencing and fluorescence in situ hybridization (FISH) are possible solutions in distinguishing homologous chromosomes, they are both costly and inefficient. In this paper, we propose a viable, cost-effective method involving indel polymorphisms on primer-isolated sequences of different strains. To distinguish homologous chromosomes by strain, size differences of these sequences will be amplified through PCR and the resulting amplicons will be run through gel electrophoresis. A dataset of mutations referenced with the N2 strain was processed to identify strain pairs with significant relative size polymorphisms. We were able to find six strain pairs with at least two regions of these polymorphisms on each chromosome. In addition, laboratory work validated our method on one region in one strain pair. We currently await further results. Nevertheless, our current results indicate that amplicon size polymorphisms are a viable, cost-effective method of distinguishing homologous chromosomes in ​C. elegans and possibly other organisms​.

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