1. Non-food applications of
Jatropha proteins
Dianika Lestari a), Wim J. Mulder b), Enny Ratnaningsih c),
Johan P. M. Sanders a)
a) Department Valorisation of Plant Production Chains, Wageningen UR, The Netherlands
b) Food & Biobased Research, Wageningen UR, The Netherlands
c) Chemistry Department, Institut Teknologi Bandung, Indonesia

2. Jatropha is an oil source for biodiesel
production
 Jatropha seed contains about 30-40% oil
 Contains toxic compounds—not suitable for food
 Can be grown in a very dry soil—not suitable for
most food plantations
 Increase productivity of the damage land
 Improve rural area development

3. Jatropha plant parts

4. Biodiesel production from Jatropha seeds
Oil Biodiesel
Seeds
Biogas
Press cake or
Fertilizer
Low value

5. Press cake contains valuable components
Carbohydrate If we can…
separate
Press cake components
Minerals
to make other
products
Fiber
We can…
Improve the
value of
Proteins press cake

6. Estimation seed added value
Value (EUR/ kg seed)
Components % Partial Total
Fractionation Fractionation
Oil 35 0.21 0.21
Crude Protein 17 0.17
Fiber/ Lignin 38 0.06
0.02
Carbohydrate 5 0.02
Ash 5 0.01
Total added value 0.23 0.48
Improve the value of seed
~2 times higher!

7. Jatropha biorefinery
Jatropha Primary Secondary Processing/ End
curcas Fractionation Fractionation Modification Product
Biodiesel
Protein
Animal
feed
Oil Technical
application
Lignin/Fiber
Binder
Pyrolysis
Seed Carbohydrate
Press cake Bioethanol

8. Project on Jatropha biorefinery
 Funded by:
Scientific Programme Indonesia – Netherlands
Royal Netherlands Academy of Arts and Sciences
(SPIN-KNAW)
 International consortium with 4 partners:
 Indonesia : BPPT, ITB
 Netherlands: RUG, Wageningen UR

9. PhD students
PPO extraction and Pharmaceutical
purification process products
E. Subroto/ Groningen University M. Insanu/ Groningen University
Oil viscosity reduction Microbial modification
press cake
L.Junistia/ Groningen University A. Marasabessy/ Wageningen UR
Novel biobased products Non-food valorization
from epoxidised oil of fibers
L. Daniel/ Groningen University H. Hidayat/ Groningen University
Somatic embryogenesis Non-food applications of
of Jatropha curcas Jatropha proteins
G. Herianto/ Groningen University D. Lestari/ Wageningen UR

10. Non-food applications of Jatropha proteins
Jatropha Jatropha
press cake proteins
Extraction
--Chapter 3--
Non-food
applications
Animal feed Technical applications
--Chapter 2-- --Chapter 5--

11. Protein
Mainly serves as
nutritional component for
foods
Also used for its
functional properties

12. Protein functional properties
Functional Food
properties applications
Emulsifying Emulsifier
properties Mayonnaise
Egg protein
Foaming Foam
properties Meringue cake
Film-forming Glaze
properties Pastry / donut
Adhesive Sugar glue
properties Cake decoration

13. In this thesis…
Functional Technical
properties applications
Emulsifying Emulsifier
properties Paint formulation
Jatropha
proteins
Foaming Foaming agent
properties Fire distinguisher
Film-forming Bioplastic
properties Packaging
Adhesive Adhesive
properties Paper glue

14. The results
Jatropha protein
Non-food / technical
Need a lot of
Coating/ bioplastic improvement
Limited
Foaming agent condition
Emulsifier
Most
promising
Paper adhesives applications

15. Conclusion and future outlooks
 Paper adhesives and industrial emulsifiers
 the two most promising applications of Jatropha proteins
 The uses of Jatropha proteins as non-food applications will
improve the market value of Jatropha seed
 lead to a more sustainable Jatropha biorefinery
 A sustainable Jatropha biorefinery will give tremendous
benefit to the society
 production of material and energy, job creation

16. Acknowledgements
Johan Sanders
Promotor
Enny Ratnaningsih
Co-promotor
Wim Mulder Department
Valorisations of Plant
Supervisor Production Chains
(VPP-WU)

17. Promovenda
Paranymph Paranymph
Dianika Lestari
Yessie Widya Sari Ischa Lamot
Opponents
Dr. Ir. M.A. Slingerland
Chair
Prof. Dr. A. Niehof
Prof. Dr. Ir. H.J. Heeres
Prof. Dr. H.P.S. Makkar Thesis Supervisor
Prof. Dr. J.P.M. Sanders
Prof. Dr. Ir. H. Gruppen

18. Thank you
For more information, contact:
•Dianika Lestari (d.lestari001@gmail.com)
•Wim J. Mulder (wim.mulder@wur.nl)
•Prof. Dr. Johan P.M. Sanders (johan.sanders@wur.nl)
© Wageningen UR www.vpp.wur.nl