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HPTLC-MS
PRESENTED BY
PROF.(DR.) DINESH KR. MEHTA
MM COLLEGE OF PHARMACY,
MM(DU), MULLANA

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HPTLC-MS
General Introduction
The direct coupling of TLC/HPTLC with mass spectrometry (MS) is of particular
interest because of the later’s high sensitivity, rapid analysis, and ability to aid
structural characterization.
TLC-MS is a versatile technique for separation as well as identification of
Pharmaceuticals and Phytopharmaceuticals. Traditionally the separation was carried
out by TLC/HPTLC then the separated materials was removed and then identified by
Mass spectrometry.
This technique provides efficient, quick and simple method for identification and
separation of Narcotic drugs and psychotropic substances.

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Key features
HPTLC–MS is cost-effective because the chromatographic run is decoupled
with the detection step.
Rapid and contamination-free elution of selected zones.
Online transfer into the mass spectrometer.
Advantages of HPTLC include that the technique is simple to learn, operate,
several analysis works could be done on same time, is a fast and economic
technique.
Thin layer chromatography/high-performance thin-layer chromatography can
be used interchangeably for methods developed in the twenty-first century.

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HPTLC-MS Principle
The versatile instrument is used to isolate unknown compounds from a
HPTLC/TLC plate and transfer them into a mass spectrometer for identification
or structure elucidation.
TLC/MS Interface can be bring together to any brand of LC-coupled mass
spectrometer.
Plug and play installation by two HPLC fittings at a given HPLC-MS system.
Semi-automatic instrument involving automatic piston movement for pressure
seal the HPTLC/TLC zone on both glass plates and aluminum foils take out
directly from the plate using a suitable solvent delivered by the HPLC/HPTLC
pump Online transfer into the mass spectrometer.
Automatic cleaning of the piston between the extractions.

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INSTRUMENTATION
A typical automated LC-MS system consists of :
Double three-way diverter in-line with an autosampler
LC system
mass spectrometer.
The diverter generally operates as an automatic switching valve to divert
undesired portions of the eluate from the LC system to waste before the sample
enters the MS.

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Fig: LC-MS (electrospray ionization interface) system
Application:
It can be used about the fragmentation patterns of degraded products

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References
Kanak Lata Verma, et al. HPTLC-MS as a Neoteric Hyphenated Technique for Separation and Forensic Identification
of Drugs, Journal of Analytical Sciences, Methods and Instrumentation, 2018, 8, 1-15
S. Nagajyothi, et al. Hyphenated Techniques- A Comprehensive Review, International Journal of Advance Research and
Development,2017.
Ashwini B. Sambherao, et al.TLC- MS HYPHENATED TECHNIQUE IN ANALYTICAL CHEMISTRY, World
Journal of Pharmacy and Pharmaceutical Sciences, Vol 6, Issue 10, 2017
HYPHENATED TECHNIQUE- A BOON TO ANALYTICAL WORLD | INTERNATIONAL JOURNAL OF
PHARMACEUTICAL SCIENCES AND RESEARCH (ijpsr.com)
Hyphenated Technique - an overview | ScienceDirect Topics
(PDF) Introduction to hyphenated techniques and their applications in pharmacy (researchgate.net)
GC × GC-MS HYPHENATED TECHNIQUES FOR THE ANALYSIS OF VOLATILE ORGANIC COMPOUNDS IN AIR: Journal of
Liquid Chromatography & Related Technologies: Vol 34, No 13 (tandfonline.com)

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Thank You