16. MG-RAST v3 workflow (simplified) SFF, fastq and fasta data find emPCR and BridgePCR artifacts find coding regions/peptides using FragGeneScan (Ye, NAR 2010) Many databases integrated GSC’s M5nr Upload QC / normalization Similarities (Parallel Blat) Metabolic reconstruction Community reconstruction Metadata Feature prediction (FGS) Abundance profiles Metabolic model
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20. Expand metadata support (2): Capture metadata early Imagine adding metadata to the plot below: Very hard after the fact! capture metadata early Aanensen et al, Plos ONE, 2009