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Folker Meyer Argonne National Laboratory and  University of Chicago June 14 th , 1 st  EMP meeting Shenzhen, China Metagenome Annotation
Metagenomics needs the magic wand.. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],data Who are they? What are they doing?
Portals help with computational analysis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
2010 state of metagenomics ,[object Object],[object Object],[object Object],[object Object]
2011: many small scale projects V3 03/2011  ,[object Object],[object Object],[object Object]
Even data upload is hard!    Jumploader Thanks to  Rob Knight ’s  team to pointing us there  
Part of an emerging digital biology ,[object Object],Source: MG-RAST, 800+ shared metagenomes
Computing cost dominate ,[object Object],Source: Rob Knight, UColorado From: Wilkening et al., IEEE Cluster09, 2009 computing sequencing
Challenges during shotgun metagenome analysis ,[object Object],[object Object],[object Object],[object Object]
Quality control  for de-novo sequencing ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Tell me if my data set is of type A or B ,[object Object],[object Object],Real data sets from MG-RAST ,[object Object],[object Object],K. Keegan, in preparation % duplicates varies also
Finding features ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Performance Analysis on simulated data sets w/ errors W. Trimble, in preparation
Characterizing features ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Presentation layer
MG-RAST v3 workflow (simplified) SFF, fastq and fasta data find emPCR and BridgePCR artifacts find coding regions/peptides using  FragGeneScan (Ye, NAR 2010) Many databases integrated GSC’s M5nr Upload QC / normalization Similarities (Parallel Blat) Metabolic reconstruction Community reconstruction Metadata Feature prediction (FGS) Abundance profiles Metabolic model
The future ,[object Object],[object Object],[object Object],Source: Rob Knight, UColorado Driving force 600 GBp / run 60 GBp / run
Future 1: World is not clonal, study strain/species variation  ,[object Object],new strain? new strain?
Future 2: Expand metadata (1): MIMS/MIMARKS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Capture metadata Extensive metadata questionnaire supporting offline editors // input
Expand metadata support (2): Capture metadata early Imagine adding metadata to the plot below: Very hard after the fact! capture metadata early Aanensen et al, Plos ONE, 2009
Many  current challenges and pitfalls ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Thank you for your attention

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Folker Meyer: Metagenomic Data Annotation

  • 1. Folker Meyer Argonne National Laboratory and University of Chicago June 14 th , 1 st EMP meeting Shenzhen, China Metagenome Annotation
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  • 6. Even data upload is hard!  Jumploader Thanks to Rob Knight ’s team to pointing us there 
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  • 13. Performance Analysis on simulated data sets w/ errors W. Trimble, in preparation
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  • 16. MG-RAST v3 workflow (simplified) SFF, fastq and fasta data find emPCR and BridgePCR artifacts find coding regions/peptides using FragGeneScan (Ye, NAR 2010) Many databases integrated GSC’s M5nr Upload QC / normalization Similarities (Parallel Blat) Metabolic reconstruction Community reconstruction Metadata Feature prediction (FGS) Abundance profiles Metabolic model
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  • 20. Expand metadata support (2): Capture metadata early Imagine adding metadata to the plot below: Very hard after the fact! capture metadata early Aanensen et al, Plos ONE, 2009
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  • 24. Thank you for your attention