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5 seun olowote effect of drying method on caroteniod content of yellow maize

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5 seun olowote effect of drying method on caroteniod content of yellow maize

  1. 1. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org EFFECT OF DRYING METHOD ON CAROTENIOD CONTENT OF YELLOW MAIZE (Zea mays) Olowote Oluwaseunab, Abe Ayodejia, Oyatomi Olaniyib , Abberton Michealb a Department of Agronomy, Faculty of Agriculture, University of Ibadan, Nigeria. b, Genetic Resource Centre, International Institute of Tropical Agriculture (IITA), Ibadan Nigeria. 22nd annual symposium on innovation agricultural research for food security and climate mitigation in sub-sahara Africa. IITA Ibadan, Oyo State. 20th -24th may, 2019.
  2. 2. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org INTRODUCTION • Maize [Zea mays (L.)] is a staple crop in several Sub Sahara Africa • Maize is commonly grown on subsistence farms
  3. 3. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org Constraints to maize production •Environmental factors, such as temperature, light exposure, and humidity reduces carotenoid content
  4. 4. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org JUSTIFICATION • Drying method applied to maize grains promotes significant reductions in the concentrations of total carotenoids, carotenes and xanthophylls in grains of maize (Wilton Soares, et al 2015) • Carotenoid content can only be retained, if it exposed to mild environmental factors (such as oxygen, temperature and light) when drying grains of maize (Moura et al., 2015).
  5. 5. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org OBJECTIVES • To determine the effect of drying methods on carotenoid content of different provitamin-A enriched maize varieties. • To determine the type of drying method that can be used for high carotenoid content retention in maize grains.
  6. 6. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org MATERIALS AND METHODS • Five provitamin-A maize varieties (IFE hybrid 3, IFE hybrid 4, PVA 13, SUWWAN 1) and a commercial check (Oba super II) obtained from maize breeding programme, IITA were used for the study • Experimental site: IITA, Ibadan • Experimental design: Randomized complete block design with three replicates
  7. 7. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org MATERIALS AND METHODS cont’d. • Plant Spacing 0.25 m x 0.75 m • Harvesting take place 55 days after pollination • Drying methods: Sun drying (35ᵒC), Air drying (20ᵒC), oven drying (35ᵒC) and non drying (control) • The 60 samples from 5 yellow maize genotypes were analyzed for their pro-vitamin A content.
  8. 8. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org MATERIALS AND METHODS cont’d. • 100 grains per samples were taken for carotenoid determination and quantification using High Performance Liquid Chromatography (HPLC)
  9. 9. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org Statistical Analysis • Analysis of variance was performed on all data using the general linear model procedure of SAS (version 9.4; SAS Institute, Cary, NC)
  10. 10. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org RESULTS Table 1. Mean squares from the analysis of variance of ten parameters evaluated for 60 samples of carotenoid maize LUT Zeax ɑ-caro 13-cis Trans 9-cis TBC T.Xan PVA (µg/g) (µg/g) (µg/g) (µg/g) (µg/g) (µg/g) (µg/g) (µg/g) (µg/g) (µg/g) Pedigree 4 25.87** 11.88** 25.11** 1.57** 1.62*** 24.18*** 2.14*** 51.43*** 18.69** 98.74*** Dry mth 3 0.87 11.51 14.46 0.03 0.49 2.28 0.99 10.06 51.55 9.65 P x D 12 1.86 5.07 12.92 0.12 0.09 2.35 0.09 3.97 29.17 6.17 LSD 0.96 1.62 3.01 0.29 0.23 1.07 0.21 1.45 3.97 1.89 CV(%) 21.05 21.78 24.53 21.24 18.07 28.04 18.44 23.14 20.20 19.88 Lutein, Zeaxanthin, β- cryptoxanthin, α-carotene, 13-cis, Trans, 9-cis, Total Beta Carotene, Total Xanthophylls, ProVitamin A.
  11. 11. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org RESULTS cont’d. 5 10 15 20 PROVITA(UG/G) Air drying Control Oven drying Sun drying DRYING METHOD Distribution of PROVITA_
  12. 12. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org RESULTS cont’d. 5 10 15 20 PROVITA(UG/G) Ifehybrid3 A irdrying Ifehybrid3 C ontrol Ifehybrid3 O ven drying Ifehybrid3 Sun drying Ifehybrid4 A irdrying Ifehybrid4 C ontrol Ifehybrid4 O ven drying Ifehybrid4 Sun drying O basuper2 A irdrying O basuper2 C ontrol O basuper2 O ven drying O basuper2 Sun drying PV A 13 A irdrying PV A 13 C ontrol PV A 13 O ven drying PV A 13 Sun drying Suw w an1 A irdrying Suw w an1 C ontrol Suw w an1 O ven drying Suw w an1 Sun drying PEDIGREE*DRYINGME Distribution of PROVITA_
  13. 13. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org CONCLUSION • Air drying under shade at 20ᵒC retained higher carotenoids content than other drying method and is recommend for the preservation and retention of high pro- vitamin A content in maize grains
  14. 14. IITA is a member of the CGIAR System Organization. www.iita.org | www.cgiar.org THANK YOU

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