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Advancements in Rodent Surgical Monitoring

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Slides from Indus Instruments's webinar (http://goo.gl/vxypa8) on the critical need for vital signs monitoring during rodent surgery, and how the MouseMonitor™ S helps improve surgical consistency, documentation, and data recording. Including presentations from doctors Corey Reynolds from Baylor College of Medicine and Merry Lindsey from the University of Mississippi Medical Center.

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Advancements in Rodent Surgical Monitoring

  1. 1. InsideScientific is an online educational environment designed for life science researchers. Our goal is to aid in the sharing and distribution of scientific information regarding innovative technologies, protocols, research tools and laboratory services.
  2. 2. Merry Lindsey, PhD Graham Sattler Rodent surgical monitoring advocate, applications specialist, and Product Manager for Indus biomedical solutions Director of the Jackson Center for Heart Research at The University of Mississippi, celebrated professor, and expert in murine models of myocardial infarction Corey Reynolds PhD Director of the Mouse Phenotyping Core Lab at Baylor College of Medicine, advanced murine model surgeon, IACUC and animal care expert
  3. 3. How do you currently document your surgery for method consistency? Lab Notebook Recording on Data Acquisition System Photos of Surgical Setup Strip Recording 0% 10% 20% 30% 40% 50% 60% 70% 80%
  4. 4. Surgical Monitoring Overview What can Surgical Monitoring do for Me? MouseMonitor S Capabilities Critical Surgical Monitoring Signals Integrated Surgical Monitoring Doesn’t Have to Be Hard
  5. 5. What Can Surgical Monitoring Do For Me? Increased Survival Rate Consistent Procedures Better Surgical Training Consistent Outcomes
  6. 6. Surgical Monitoring Essentials… Non-Invasive 6-Lead ECG Blood Oxygenation Monitoring Respiration Monitoring Responsive Heating Core Temperature Measurement
  7. 7. Critical Surgical Monitoring Signals Multiple physiologic signals must be monitored to effectively assess subject health Monitor… Heart Rate Core Temperature Blood Oxygenation Maintain… Safe Core Temperature
  8. 8. Signal Integration… …it doesn’t have to be hard ECG, Heart Rate & Respiration Rate from a single set of electrodes Perform Surgeries directly on Stainless Steel top Intuitive Touch Screen Controls Factory Presets Enable Out of Box Operation All Signals Captured Non-Invasively
  9. 9. Rodent Surgical Monitoring In Review Comprehensive monitoring offers greater consistency and confidence Consistent Interventions + Higher Survival Rate = Better Outcomes The MouseMonitor S is the only preclinical research device that measures every key vital sign in one device Click Here For More Information
  10. 10. How important do you view rodent surgical monitoring? Very Important Important Somewhat Important 0% 10% 20% 30% 40% 50% 60%
  11. 11. What does the MPC Offer? The Mouse Phenotyping Core is a comprehensive facility offering expertise in the following areas: Imaging Metabolic Assays Cardiovasuclar Assays Respiratory Assays Neuromuscular Assessment Surgical services
  12. 12. What does the MPC Offer? MPC Cont. Fee-for-service Core located at BCM Can accept animals (rats/mice) from outside facilities post veterinary approval Can run long term studies and provide data analysis Contact Information: Corey L. Reynolds 713-798-5040--------Clreynol@bcm.edu
  13. 13. Challenges in a Core Lab… Species Clients/PIs Many Different Procedures Protocols
  14. 14. Recommendations By The NRC Guide for the Care and Use of Laboratory Animals , 8th ed. Providing Artificial Heating (p119) Monitoring baseline vital signs, including: Heart Rate (p119) Core Temperature (p119) Blood Oxygenation (p122)
  15. 15. What a Core Lab Needs… Core temperature monitoring & adjustable heating. ECG/Heart Rate electrode flexibility for different-sized species. Blood Oxygenation (SpO2) monitoring. Compact Comprehensive Surgical monitoring.
  16. 16. Advantages of Integrated Monitoring & Recording Easy Screenshots Easy Recordings
  17. 17. Advantages of Integrated Monitoring & Recording Constant Monitoring of Animal Vitals During Surgery Documentation of Monitoring Very Liked by IACUC committee.
  18. 18. Applications In The Mouse Phenotyping Core Multiple Hi-Resolution ECG leads enable easier Morphology Recognition & Arrhythmia Detection Sinus Rhythm with Bigeminal PVCs
  19. 19. Applications In The Mouse Phenotyping Core Serial Ultrasound (Vevo) B-Mode and ECG capture for analysis ECG data integrates effectively with other equipment as well
  20. 20. What factors influence how frequently you utilize surgical monitoring? My current setup takes too much time to use I am working on setting up my lab I don’t currently have suitable monitoring equipment My current setup doesn’t work properly 0% 5% 10% 15% 20% 25% 30% 35%
  21. 21. Myocardial Infarction (by Left Coronary Artery Decending (LAD) Ligation)C Universal definition of myocardial infarction. Kristian Thygesen, Joseph S. Alpert and Harvey D. White on behalf of the Joint ESC/ACCF/AHA/WHF Task Force for the Redefinition of Myocardial Infarction. Eur Heart J (2007) 28 (20): 2525-2538.
  22. 22. Post-MI Cardiac Remodeling Yabluchanskiy et al. Congest Heart Fail. 2013
  23. 23. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling
  24. 24. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling
  25. 25. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling
  26. 26. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling
  27. 27. Lindsey Lab – Current Research Areas Effects of MMP-9 deletion in aging mice post-MI Effects of MMP-9 deletion post-MI in mice with P. gingivalis infection Effects of MMPs derived peptides in vitro and in vivo Effects of selective MMP inhibitors post-MI
  28. 28. ECG Before LAD Ligation
  29. 29. ECG 5 Min After LAD Ligation
  30. 30. Critical Needs in our Lab For In Vivo Models Reliable & accurate ECG recording Comfortable size of the pad Multiple leads choice External temperature probe External and internal ECG electrodes Portable tablet system Controlled heated pad Data management/export
  31. 31. Thank you for taking part in this event. We encourage all attendees to register at www.insidescientific.com for notifications about future webinars. WEBSERIES COMING SOON… PV Loops to Measure Cardiac Function Implantable Biotelemetry Non-Invasive Blood Pressure Monitoring

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