2. Presented By
Md. Rezaur Rahman
REGISTRATION NO.: 1305099
Supervisor
Dr. Md. Faruk Islam
Associate Professor
DEPARTMENT OF MEDICINE, SURGERY AND OBSTETRICS
HAJEE MOHAMMAD DANESH SCIENCE & TECHNOLOGY
UNIVERSITY, DINAJPUR
SEROPREVALANCE AND EFFICACY OF
COMMERCIAL VACCINE AGAINST NEWCASTLE
DISEASE IN LAYER BIRDS
Co Supervisor
Dr. Md. Shajedur
Rahman
Assistant Professor
3. Newcastle Disease is one of the most important
infectious disease of poultry in Bangladesh
It is still considered a major problem, because
of its high morbidity, mortality particularly in
rural areas and reduce productivity and
economic loss in layer farm
Velogenic Strain Cause Mortality up to 90%
and Morbidity near 100%
Prevalence of ND in different areas of
Bangladesh are 50%, 45%, 25% and 32.5%.
BACKGROUND
4. Determination of seroprevalence of Newcastle
Disease (ND) by Hamagglutination Inhibition (HI)
test
Evaluation of immune response of different
commercial vaccines in grower and layer birds.
OBJECTIVES
6. Study area:
The study was carried out at different layer
farms of three upazilla namely- Kaharol,
Bochagonj and Setabgonj in Dinajpur district
Total Number of Sample:
20 blood samples from each farm
Study period:
January-June,, 2015
MATERIALS & METHODS
7. Study farm and population:Study farm and population:
Six (06) farms and total population of birds are
6000
Laboratory examination:
Toufiq Agro Laboratory, RangpurToufiq Agro Laboratory, Rangpur
MATERIALS & METHODS
8. SL
No.
Name of
Vaccine
Strain Company
01 Avinew®
VG/GA Strain Merial
02 Cevac New L®
LaSota strain ACI
03 Newcastle
Disease vaccine®
Mesogenic
strain
Novartis
MATERIALS & METHODS
9. Phosphate Buffer Solution (PBS)
Newcastle Disease Vaccine
Experimental Chicken
Disposable Syringe
Micropipette
96 Well micro titre plate
Glassware and Appliances
Chicken Red Blood Cell (cRBC) Suspension
MATERIALS
MATERIALS & METHODS
12. Select of farmsSelect of farms
Collection of 20 blood sample from each farm
for Haemagglutination inhibition (HI) test to
know Ab titire
Collection of 20 blood sample from each farm
for Haemagglutination inhibition (HI) test to
know Ab titire
HI perform at Toufic agro lab. To determine the
Seroprevalence
HI perform at Toufic agro lab. To determine the
Seroprevalence
Selection of farms for vaccinationSelection of farms for vaccination
EXPERIMENTAL LAYOUT
13. Vaccination:
• Farm A --- New Castle Diseases Vaccine
(Mesogenic Strain)
• Farm C --- Avinew (VG/GA Strain)
• Farm D--- Cevac new-L (Lasota)
Vaccination:
• Farm A --- New Castle Diseases Vaccine
(Mesogenic Strain)
• Farm C --- Avinew (VG/GA Strain)
• Farm D--- Cevac new-L (Lasota)
20 blood serum from each farm for
determination of Ab titre (20 × 3 = 60)
at Day7 and Day14
20 blood serum from each farm for
determination of Ab titre (20 × 3 = 60)
at Day7 and Day14
Evaluation and comparison of vaccine
effectiveness against ND
Evaluation and comparison of vaccine
effectiveness against ND
EXPERIMENTAL LAYOUT
15. Table A. Seroprevalence of Newcastle Disease in grower
farms
Ab = Antibody
Farms
Grower birds
Overall
prevalence
Age
of
birds
(wks)
Total
serum
tested
Mean
Ab titre
level
Positive
case
Prevalence
among
tested
cases
A 16 20 23.6
5 25%
18.33%B 12 20 28.9
0 0%
C 14 20 23.9
6 30%
17. Table C. Ab titre level of 3 farms at Day0 (Day of vaccination)
(HI test at experimental “Day 0” at Geometric mean log2)
(C-Mamun poultry, D- Masud poultry, A- Rakib poultry)
Farms Stage
No. of blood
serum sample
tested
Mean of Titre
(Before
Vaccination)
Farm-C Grower 20 23.9
Farm-A Layer 20 25.6
Farm-D Grower 20 23.6
18. Table D. Evaluation of vaccine efficacy after Day 7 and Day 14 day of
following vaccination by different vaccine in different ages layer farms
(Farm C-Mamun poultry, D- Masud poultry, A- Rakib poultry)
19. Table E. Comparative study of Immune response at
grower farms
NS= Not Significance
Farms
(grower)
Vaccine name
HI (D7
) titre
After Vaccination
HI (D14
) titre
After
Vaccination
A
Avinew (VG/GA
strain)
27.6
28.1
C
Newcastle disease
vaccine
(Mesogenic strain)
27.2
28.0
P Value (t-test) NS NS
20. CONCLUSIONS
Detection of seroprevalance of ND in layer birds may
assist to reduced prevalence of ND in layer farm through
proper vaccination.
The titre level in farm C (Avinew VG/GA strain), A
(Mesogenic strain) and D (Cevacnew-Lasopa) on Day7 and
Day14 was 27.2
,28
; 27.6
, 28.1
and 29.1
, 29.8
, respectively in
three farms
Cevac New L (LaSota strain) may be the choice of vaccine
against ND for layer farms though others may also be
used.
Further study may need to conduct in large scale of birds
using all commercial vaccine for recommendation.
