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FOOD TECHNOLOGY 
Bacteria – Linked with Micro-organisms in Food 
Production
SALMONELLA 
 Is a genus of rod-shaped, Gram negative, spore forming 
bacteria. 
 Incubation period: 12-72 hours 
 Duration f Illness: 4-7 days but can cause more serious illness 
in older adults, infants and persons with chronic diseases. 
 Symptoms: Diarrhoea, fever, abdominal cramps and vomiting 
 Sources: Contaminated eggs, poultry, meat, unpasteurised 
milk and juice, cheese, contaminated raw fruits and 
vegetables. 
To prevent contamination: 
 Cook foods above the ‘danger zone’ (5-65°C) to kill micro-organisms 
 Wash hands thoroughly 
 Keep food properly refrigerated 
 Separate cooked and uncooked food, and use separate 
utensils for each.
LISTERIA MONOCYTOGENES 
 Is a Gram-positive bacterium found in soil and water and some 
animals, including poultry and cattle. 
 Incubation period: 3-70 days 
 Duration to illness: Days to weeks 
 Symptoms: Fever, stiff neck, confusion, vomiting, sometimes 
diarrhoea. 
 Sources: Refrigerated pates or meat spreads, unpasteurised milk 
and day products, raw sprouts, ready-to-eat deli meats and hot 
dogs. 
 Who’s at risk: Older adults, pregnant women, people with certain 
diseases (cancer, liver disease, HIV/AIDS). Due to frequent 
pathogenetically, causing meningitis in newborns, pregnant mothers 
are advised not to eat soft cheeses (brie and feta for example). 
To prevent contamination: 
 Do not drink raw (unpasteurized) milk, and do not eat foods that 
have unpasteurized milk in them. 
 Wash hands, countertops, and utensils after handling and preparing 
uncooked foods. 
 Keep uncooked meats, poultry separate from vegetables, fruits, 
cooked foods, and ready-to-eat foods.
CAMPYLOBACTER 
 Is a genus of bacteria that are Gram-negative, spiral in shape 
and microaerophilic. 
 Sources: Raw and uncooked poultry, unpasteurised milk, 
contaminated water. 
 Symptoms: Diarrhoea, cramps, fever and vomiting, diarrhoea 
may be bloody. 
 Incubation period: 2-5 days 
 Duration of illness: 2-10 days. 
To prevent contamination: 
 Always cook meat, especially poultry, to safe minimum 
temperatures. 
 Keep raw meat separate from other foods. 
 Do not drink unpasteurised milk.
STAPHYLOCOCCUS AUREUS 
 Type of bacteria commonly found on the skin and hair as well 
as in the noses and throats of people and animals. Cause 
food poisoning when a food handler contaminates food and 
then the food is not properly refrigerated. 
 Sources: Salads, such as ham, egg, tuna, chicken, potato, 
and bakery products, such as cream-filled pastries and cream 
pies 
 Incubation period: 1-6 hours 
 Duration of illness: 24-48 hours 
 Symptoms: Nausea, vomiting, diarrhoea, loss of appetite, 
sever abdominal cramps, mild fever 
To prevent contamination: 
 Wash hands and under fingernails vigorously with soap and 
water before handling and preparing food. 
 Keep kitchens and food-serving areas clean and sanitized. 
 Store cooked food in a wide, shallow container and refrigerate 
as soon as possible.
ESCHERICHIA COLI (E COLI) 
 Is a Gram-negative bacillus which can grow both aerobically 
and anaerobically. 
 Sources: Gut of humans and warm-blooded animals, sewage, 
water and raw meat. 
 Symptoms: Abdominal cramps, vomiting, diarrhoea and in 
some people haemorrhagic colitis (bleeding of the bowel). 
Occasionally however, particularly in young children, 
haemolytic uraemic syndrome (HUS) develops. This is a 
condition which leads to kidney failure and death is 5-10% o 
cases. HUS takes 1 – 2 weeks to develop. 
 Incubation period: 1-6 days 
 Duration of Illness: 5-10 days 
To prevent contamination: 
 Hygienic slaughtering practices reduces contamination of 
carcasses by faeces 
 Vulnerable populations (e.g. Children or Elderly) should avoid 
consumption of raw/ undercooked meat or milk/ milk products. 
 Cook all meat to at least 72°C. 
 Wash fruit and vegetables.
CLOSTRIDIUM PERFRINGENS 
 It is an anaerobic, spore forming bacillus. Food poisoning caused by 
this bacterium is due to eating a food containing large numbers of 
living bacteria which subsequently release a toxin in the intestine. 
 Sources: Raw meat and poultry – bacteria from raw meat may 
spread to cooked food through cross contamination. Food handlers, 
insects and reheated food can contaminate. 
 Symptoms: Nausea, Abdominal pains, Diarrhoea and Vomiting 
(although this is rare) 
 Incubation period: 8-22 days 
 Duration of Illness: 12-48 hours 
To prevent contamination: 
 Since this bacteria form heat-resistant spores it cannot be assumed 
that the bacteria are killed during cooking. In order to lower the risk 
of clostridium perfringers, the following precautions should be taken: 
 Joints of meat (+3Kg) should be cut into smaller pieces before 
cooking. 
 Raw and cooked meats stored separately. 
 Reheating of meat should be avoided if possible. If necessary the 
meat should be heated and served quickly.
CLOSTRIDIUM BOTULINUM 
 A Gram-positive, anaerobic, spore-forming bacillus. 
 Produces an exotoxin whilst growing in food and is one of the 
most lethal poisons known. 
 Illness caused by the organism Botulism. 
 Sources: Soil, some fish and vegetables and some canned, 
bottled and vacuum packed foods. 
 Symptoms: Toxin affects central nervous system. Early 
symptoms include double vision, difficulty with speech, 
headache and dizziness. 
 Incubation period: 18-36 hours 
 Duration of Illness: 1-8 days 
To prevent contamination: 
 Botulinum toxin is destroyed by heat. Heating to 80°C for 15 
minutes is sufficient to destroy the toxin.
BACILLUS CEREUS 
 A Gram-positive, spore-forming, aerobic bacillus. 
 It produces a heat-resistant exotoxin which is released into 
food. The toxin can survive for 1.5 hours at 121°C. 
 Sources: Soil, dust and water. Frequently present in cereal 
foods – particularly rice and cornflour. Some rice dishes 
(boiled and fried) and milk puddings. 
 Symptoms: Vomiting, abdominal pains and some diarrhoea. 
 Incubation period: 1-6 hours 
 Duration of Illness: 6-24 hours 
To prevent contamination: 
 Rice dishes, milk puddings and cornflour sauces should be 
cooled quickly (within 90 minutes). 
 Re-heating food should be heated quickly and thoroughly and 
served as soon as possible.

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Food Technology Bacteria

  • 1. FOOD TECHNOLOGY Bacteria – Linked with Micro-organisms in Food Production
  • 2. SALMONELLA  Is a genus of rod-shaped, Gram negative, spore forming bacteria.  Incubation period: 12-72 hours  Duration f Illness: 4-7 days but can cause more serious illness in older adults, infants and persons with chronic diseases.  Symptoms: Diarrhoea, fever, abdominal cramps and vomiting  Sources: Contaminated eggs, poultry, meat, unpasteurised milk and juice, cheese, contaminated raw fruits and vegetables. To prevent contamination:  Cook foods above the ‘danger zone’ (5-65°C) to kill micro-organisms  Wash hands thoroughly  Keep food properly refrigerated  Separate cooked and uncooked food, and use separate utensils for each.
  • 3. LISTERIA MONOCYTOGENES  Is a Gram-positive bacterium found in soil and water and some animals, including poultry and cattle.  Incubation period: 3-70 days  Duration to illness: Days to weeks  Symptoms: Fever, stiff neck, confusion, vomiting, sometimes diarrhoea.  Sources: Refrigerated pates or meat spreads, unpasteurised milk and day products, raw sprouts, ready-to-eat deli meats and hot dogs.  Who’s at risk: Older adults, pregnant women, people with certain diseases (cancer, liver disease, HIV/AIDS). Due to frequent pathogenetically, causing meningitis in newborns, pregnant mothers are advised not to eat soft cheeses (brie and feta for example). To prevent contamination:  Do not drink raw (unpasteurized) milk, and do not eat foods that have unpasteurized milk in them.  Wash hands, countertops, and utensils after handling and preparing uncooked foods.  Keep uncooked meats, poultry separate from vegetables, fruits, cooked foods, and ready-to-eat foods.
  • 4. CAMPYLOBACTER  Is a genus of bacteria that are Gram-negative, spiral in shape and microaerophilic.  Sources: Raw and uncooked poultry, unpasteurised milk, contaminated water.  Symptoms: Diarrhoea, cramps, fever and vomiting, diarrhoea may be bloody.  Incubation period: 2-5 days  Duration of illness: 2-10 days. To prevent contamination:  Always cook meat, especially poultry, to safe minimum temperatures.  Keep raw meat separate from other foods.  Do not drink unpasteurised milk.
  • 5. STAPHYLOCOCCUS AUREUS  Type of bacteria commonly found on the skin and hair as well as in the noses and throats of people and animals. Cause food poisoning when a food handler contaminates food and then the food is not properly refrigerated.  Sources: Salads, such as ham, egg, tuna, chicken, potato, and bakery products, such as cream-filled pastries and cream pies  Incubation period: 1-6 hours  Duration of illness: 24-48 hours  Symptoms: Nausea, vomiting, diarrhoea, loss of appetite, sever abdominal cramps, mild fever To prevent contamination:  Wash hands and under fingernails vigorously with soap and water before handling and preparing food.  Keep kitchens and food-serving areas clean and sanitized.  Store cooked food in a wide, shallow container and refrigerate as soon as possible.
  • 6. ESCHERICHIA COLI (E COLI)  Is a Gram-negative bacillus which can grow both aerobically and anaerobically.  Sources: Gut of humans and warm-blooded animals, sewage, water and raw meat.  Symptoms: Abdominal cramps, vomiting, diarrhoea and in some people haemorrhagic colitis (bleeding of the bowel). Occasionally however, particularly in young children, haemolytic uraemic syndrome (HUS) develops. This is a condition which leads to kidney failure and death is 5-10% o cases. HUS takes 1 – 2 weeks to develop.  Incubation period: 1-6 days  Duration of Illness: 5-10 days To prevent contamination:  Hygienic slaughtering practices reduces contamination of carcasses by faeces  Vulnerable populations (e.g. Children or Elderly) should avoid consumption of raw/ undercooked meat or milk/ milk products.  Cook all meat to at least 72°C.  Wash fruit and vegetables.
  • 7. CLOSTRIDIUM PERFRINGENS  It is an anaerobic, spore forming bacillus. Food poisoning caused by this bacterium is due to eating a food containing large numbers of living bacteria which subsequently release a toxin in the intestine.  Sources: Raw meat and poultry – bacteria from raw meat may spread to cooked food through cross contamination. Food handlers, insects and reheated food can contaminate.  Symptoms: Nausea, Abdominal pains, Diarrhoea and Vomiting (although this is rare)  Incubation period: 8-22 days  Duration of Illness: 12-48 hours To prevent contamination:  Since this bacteria form heat-resistant spores it cannot be assumed that the bacteria are killed during cooking. In order to lower the risk of clostridium perfringers, the following precautions should be taken:  Joints of meat (+3Kg) should be cut into smaller pieces before cooking.  Raw and cooked meats stored separately.  Reheating of meat should be avoided if possible. If necessary the meat should be heated and served quickly.
  • 8. CLOSTRIDIUM BOTULINUM  A Gram-positive, anaerobic, spore-forming bacillus.  Produces an exotoxin whilst growing in food and is one of the most lethal poisons known.  Illness caused by the organism Botulism.  Sources: Soil, some fish and vegetables and some canned, bottled and vacuum packed foods.  Symptoms: Toxin affects central nervous system. Early symptoms include double vision, difficulty with speech, headache and dizziness.  Incubation period: 18-36 hours  Duration of Illness: 1-8 days To prevent contamination:  Botulinum toxin is destroyed by heat. Heating to 80°C for 15 minutes is sufficient to destroy the toxin.
  • 9. BACILLUS CEREUS  A Gram-positive, spore-forming, aerobic bacillus.  It produces a heat-resistant exotoxin which is released into food. The toxin can survive for 1.5 hours at 121°C.  Sources: Soil, dust and water. Frequently present in cereal foods – particularly rice and cornflour. Some rice dishes (boiled and fried) and milk puddings.  Symptoms: Vomiting, abdominal pains and some diarrhoea.  Incubation period: 1-6 hours  Duration of Illness: 6-24 hours To prevent contamination:  Rice dishes, milk puddings and cornflour sauces should be cooled quickly (within 90 minutes).  Re-heating food should be heated quickly and thoroughly and served as soon as possible.