Se ha denunciado esta presentación.
Utilizamos tu perfil de LinkedIn y tus datos de actividad para personalizar los anuncios y mostrarte publicidad más relevante. Puedes cambiar tus preferencias de publicidad en cualquier momento.

0

Compartir

In Vivo Imaging in Alopecia Areata Patients Using Multiphoton Microscopy

Exploring the utility of multiphoton microscopy (MPM) as an adjunct to histological studies and a diagnostic tool for alopecia areata.

Audiolibros relacionados

Gratis con una prueba de 30 días de Scribd

Ver todo
  • Sé el primero en recomendar esto

In Vivo Imaging in Alopecia Areata Patients Using Multiphoton Microscopy

  1. 1. In Vivo Imaging in Alopecia Areata Patients Using Multiphoton Microscopy Jessica Lin,BSc1, Inga Saknite,PhD2, Manuel Valdebran,MD2, Mihaela Balu,PhD2, Griffin Lentsch,MSc2, Joshua N. Williams,BSc2, Karsten Koenig,PhD2, Bruce J. Tromberg,PhD2, and Natasha Atanaskova Mesinkovska,MD, PhD1,2 1Department of Dermatology, University of California, Irvine, California 2Laser Microbeam and Medical Program, Beckman Laser Institute, Irvine, California
  2. 2. Financial Disclosure 2 I have no financial interests to disclose in relation to this presentation
  3. 3. Clinical Diagnosis • Pattern of hair loss • Exclamation point hairs • Dermoscopy – Yellow dots 3 Soutor C, Hordinsky MK. Clinical Dermatology; 2017
  4. 4. Diagnosis of Alopecia Areata by Histology – Gold Standard 4 Normal Acute Chronic Sperling, Leonard C., Shawn E. Cowper, and Eleanor A. Knopp. An atlas of hair pathology with clinical correlations. CRC Press, 2012.
  5. 5. Non-invasive Optical Imaging in Trichology • Ultrasound • Optical Coherence Tomography • Laser Scanning Microscopy 5 MPM 200 μm 1100 μm
  6. 6. Multiphoton Microscopy (MPM) • Laser Scanning Microscopy • Illumination: – Titanium sapphire laser – Spectral wavelength between 710 – 920 nm – Focused femtosecond laser pulses • Endogenous autofluorophores 6
  7. 7. Endogenous Autofluorophores 7Pena A, Strupler M, Boulesteix T, Schanne-Klein M. Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy. Opt Express. 2005 Aug 8;13(16):6268-74.
  8. 8. Contrast Mechanism: intrinsic fluorescence combined with second harmonic generation 8M G Blanchin 2015 IOP Conf. Ser.: Mater. Sci. Eng. 80 012027 2 photon emission (2PE) 2nd harmonic generation (SHG)
  9. 9. Zipfel WR, Williams RM, Christie R, Nikitin AY, Hyman BT, Webb WW. Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7075-80. Epub 2003 May 19. Autofluorophores - 2 photon emission (2PE)
  10. 10. Collagen is a nonlinear medium – 2nd harmonic generation 10
  11. 11. 11 z = 0 μm z = 20 μm z = 40 μm z = 80 μm z = 100 μm z = 140 μm Images from Patient 1 (36 Male Alopecia Universalis) Epidermis Dermal-Epidermal Junction Dermis
  12. 12. Hair Follicle 12 Z=65µm collagen Hair shaft keratinocytes elastin keratinocytes Hair follicle Z=5µm
  13. 13. Cellular Details of Keratinocytes nucleus cytoplasm Z=15µm
  14. 14. Exclamation point hair Image from Patient 1 (36 Male Alopecia Universalis)
  15. 15. Horizontal and vertical sections of same area SC SS SB 30 μm z = 0 μm z = 20 μm z = 40 μm z = 80 μm z = 100 μm z = 140 μm Image from Patient 1 (36 Male Alopecia Universalis)
  16. 16. Normal vs. Alopecia Universalis Miniaturized Hair 16 Z = 65 AUNormal Z = 65
  17. 17. Same Pattern in 3 separate areas 17 Z = 65 Z = 65Z = 65 Images from 41 Female with AU
  18. 18. Collagen patterns in scarring vs. non-scarring alopecia at same depth 18 Z = 75 Z = 75 FFA AU
  19. 19. Perifollicular inflammation 19 Z = 95 Image from Patient 1 (36 Male Alopecia Universalis)
  20. 20. Dilated follicular infundibula filled with orthokeratin 20 Müller CS, El Shabrawi-Caelen L. 'Follicular Swiss cheese' pattern--another histopathologic clue to alopecia areata. J Cutan Pathol. 2011 Feb;38(2):185-9. Khopkar U, Jain N. Chapter 4 – Trichoscopy: The Dermatologist's Third Eye. Imaging in Dermatology, 2016, 29–41. Dermoscopy MPM Histology SC SS SB
  21. 21. Follicular Measurements 21
  22. 22. Follicle and Shaft Sizes 22
  23. 23. Clinical Implications • Bedside Diagnosis • Salvage of Hair Follicles from Biopsy • Monitor Treatment 23
  24. 24. Next Steps • Standardize imaging protocol • Establish diagnostic criteria • Creating nomenclature and vocabulary • Unique and concurrent features • Tuning for other tissue components 24
  25. 25. Conclusion • MPM is useful for research and clinical use – Dermatology  Trichology – Morphological tool – Diagnosis of alopecia – Research
  26. 26. Thank you Jessica Lin Email: linj19@uci.edu

Exploring the utility of multiphoton microscopy (MPM) as an adjunct to histological studies and a diagnostic tool for alopecia areata.

Vistas

Total de vistas

246

En Slideshare

0

De embebidos

0

Número de embebidos

0

Acciones

Descargas

0

Compartidos

0

Comentarios

0

Me gusta

0

×