Learning Objectives:
Define Microscopy
Trace the History of the Microscope
Identify and label the parts of microscope
Distinguish the magnifying, illuminating and
mechanical parts of the microscope
State the importance of microscope in our present
situation
MICROSCOPY
the technical field of using microscopes to
view objects and areas of objects that cannot
be seen with the naked eye (objects that are
not within the resolution range of the normal
eye).
Activity # 1: Study the letter-maze below. Look for the six
parts of the microscope hidden on the letter-maze.
What Do You Think?
Can microorganisms be seen with the naked eye?
If not, how can they be seen?
Why is the microscope important to the study of
Biology?
MICROSCOPE
micron – small +
skopein – to look up
optical instrument that
uses lens or a combination
of lenses used for viewing
small objects not visible to
the naked eye.
MAGNIFYING PARTS
Use to see and enlarge specimen
Includes:
Eyepiece/ocular- allows you to
look through and observe the
enlarge image of the object
Objective Lenses - magnify the
image and responsible for quality
of the image
THREE TYPES OF OBJECTIVES
Low-power objective lens - shorter
than the other objectives and
magnifies about 10x. Its function is
to locate and show the outline of
the specimen
High-power objective lens – long
and slender and magnifies about
40x or 60x. It shows the detailed
structure of the specimen
Oil Immersion objective lens – may
be long or short. Usually marked 99x
or 100x. Cedar oil is used on the
outside lens to show the specimen
very well
ILLUMINATING PARTS
Used to supply light to the
slide on the stage
Includes:
Diaphragm - which
controls the passing of light
in the opening of the stage
Mirror or an electric
lamp/light bulb - used to
provide light on the object
and help focuses it.
MECHANICAL PARTS
Used to support and operate the parts
Includes:
course adjustment knob- which is used
to move the tube and the objective
lenses closer or farther away from the
stage to view the image of the object,
use to focus the LPO.
fine adjustment knob- which moves the
stage up and down and brings the
image of the object into fine focus
producing a clearer and more detailed
view of the object.
MECHANICAL PARTS
STAGE – Place where the specimen is
mounted
Stage Clip- hold the slide in place
Arm - the part of
the microscope that connects the
eyepiece tube to the base.
Base – Used to anchor the
microscope firmly on the table
Revolving nosepiece - allows proper
position the objectives lenses.
Body Tube - holds the eyepiece and
the revolving nosepiece in place.
Aside from knowing about the essentials of the
microscope and its functions, it is also important to learn
and practice how to take good care of this instrument. You
should always follow the guidelines on how to maintain the
good working condition of the microscope. If you are given
the opportunity to use it, handle it properly and clean its
parts using the appropriate materials. Return it to its proper
casing and store it in a clean and dry storage cabinet away
from laboratory chemicals.
How to Clean and Keep the Microscope
Gently wipe the lens with lens paper
Replace the cover of the microscope after use
Clean and inspect the microscope before and after use
Always carry the microscope with one hand supporting
the base and the other hand around the arm
Read the following situations. Identify the part and the
function of the microscope mentioned in each situation.
In this time of CoVID19 Pandemic, how
useful is the microscope in detecting the
viruses? What kind of microscope is
being used in studying this kind of virus?
1. It is held together, together with the base, when
carrying the microscope
a. Stage
b. Arm
c. Body tube
d. Objective lens
2. This part is where you place the specimen
a. Stage
b. Eyepiece
c. Objective lens
d. Stage clips
Which of the following would you use to study plant cell
a. Kaleidoscope
b. Microscope
c. Telescope
d. Stethoscope
Which two parts of microscope magnify the image of an
object?
a.Eyepiece and mirror
b.Objective lens and mirror
c. Eyepiece and Objectives
d.Objectives and Diaphragm
Which of these part functions as magnifying part?
a.Base
b.Adjustment knob
c. Mirror
d.Ocular eyepiece
The microscope is an important tool in the study of living things. It contains lens or a combination of lenses to produce highly magnified or enlarged images of small objects or specimens. A light source could be the sunlight through the mirror or from an electric light bulb