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The resolution and performance of an optical microscope can be characterized by a quantity known as the modulation transfer function (MTF), which is a measurement of the microscope's ability to transfer contrast from the specimen to the intermediate image plane at a specific resolution.
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Introduction The applications of microscopy in the forensic sciences are almost limitless. This is due in large measure to the ability of microscopes to detect, resolve and image the smallest items of evidence, often without alteration or destruction. As a result, microscopes have become nearly indispensable in all forensic disciplines involving the natural sciences. Thus, a firearms examiner comparing a bullet, a trace evidence specialist identifying and comparing fibers, hairs, soils or dust, a document examiner studying ink line crossings or paper fibers, and a serologist scrutinizing a bloodstain, all rely on microscopes, in spite of the fact that each may use them in different ways and for different purposes. The principal purpose of any microscope is to form an enlarged image of a small object. As the image is more greatly magnified, the concern then becomes resolution; the ability to see increasingly fine details as the magnification is increased. For most observers, the ability to see fine details of an item of evidence at a convenient magnification, is sufficient. For many items, such as ink lines, bloodstains or bullets, no treatment is required and the evidence may typically be studied directly under the appropriate microscope without any form of sample preparation. For other types of evidence, particularly traces of particulate matter, sample preparation before the microscopical examination begins is often essential. Types of Microscopes Used in the Forensic Sciences A variety of microscopes are used in any modern forensic science laboratory. Most of these are light microscopes which use photons to form images, but electron microscopes, particularly the scanning electron microscope (SEM), are finding applications in larger, full service laboratories because of their wide range of magnification, high resolving power and ability to perform elemental analyses when equipped with an energy or wavelength dispersive X-ray spectrometer.
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Introduction The applications of microscopy in the forensic sciences are almost limitless. This is due in large measure to the ability of microscopes to detect, resolve and image the smallest items of evidence, often without alteration or destruction. As a result, microscopes have become nearly indispensable in all forensic disciplines involving the natural sciences. Thus, a firearms examiner comparing a bullet, a trace evidence specialist identifying and comparing fibers, hairs, soils or dust, a document examiner studying ink line crossings or paper fibers, and a serologist scrutinizing a bloodstain, all rely on microscopes, in spite of the fact that each may use them in different ways and for different purposes. The principal purpose of any microscope is to form an enlarged image of a small object. As the image is more greatly magnified, the concern then becomes resolution; the ability to see increasingly fine details as the magnification is increased. For most observers, the ability to see fine details of an item of evidence at a convenient magnification, is sufficient. For many items, such as ink lines, bloodstains or bullets, no treatment is required and the evidence may typically be studied directly under the appropriate microscope without any form of sample preparation. For other types of evidence, particularly traces of particulate matter, sample preparation before the microscopical examination begins is often essential. Types of Microscopes Used in the Forensic Sciences A variety of microscopes are used in any modern forensic science laboratory. Most of these are light microscopes which use photons to form images, but electron microscopes, particularly the scanning electron microscope (SEM), are finding applications in larger, full service laboratories because of their wide range of magnification, high resolving power and ability to perform elemental analyses when equipped with an energy or wavelength dispersive X-ray spectrometer.
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