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Nmr spectroscopy
1. NMR SPECTROSCOPY
Submitted to -:
Dr.Monika Asthana
Dept.of Biotechnology
school of life science,
DBRAU Agra.
Submitted by-:
Prashant sharma
biotechnologist
2.
3. INTRODUCTION
NMR spectroscopy stands for nuclear magnetic
resonance spectroscopy
NMR spectroscopy is a analytical technique which is
used in characterizing organic molecules by
identifying carbon hydrogen frameworks
This technique utilizes the magnetic properties of
some nmr active substances
Nmr spectroscopy works on the spin of the nucleus as
well as the standard absobtion phenomenon of
spectroscopy
4. HISTORY OF NMR
The Purcell group at Harvard University and the Bloch
group at Stanford University independently developed
NMR spectroscopy in the late 1940s and early 1950s.
Edward Mills Purcell and Felix Bloch shared the
1952 Nobel Prize in Physics for their discoveries
Prof. R.R. Ernst received the Nobel Prize in Chemistry,
in 1991, for the development of the NMR techniques
5. TYPES OF NMR
There are various types of NMR SPECTROSCOPY but
the most common are of two types which are
charachterized on the basis of atoms used
1 H1 NMR -: it is used to study the types and number of
H atoms
2 C13 NMR-: it is used to study the types of different
carbon atoms
6. Source of NMR
The source of nmr is radio waves which have a ong
wavelength having more than 107 nm and thus low
frequency and energy
When these low energy waves interact with a molecule
they can change the nuclear spins of the some
elements including 1H and 13C
7. PRINCIPLES OF NMR
The principle behind NMR comes from
The spin of the nucleus and it generates a magnetic field without an
external applied magnetic field, the nuclear spins are random in
directions
But when an external field is applied the nuclei align themselves in the
direction of the magnetic field or opposite this is called spin flip
8. If an external field is applied , an energy transfer is
possible between ground state to excited state
When the spin returns to the ground state the
absorbed radiofrequency energy is emitted at the same
frequency level
This emitted radio frequency gives the nmr spectrum
of the concerned nucleus
11. INSTRUMENTATION
1.Sample holder 4.sweep generator
2permanent magnet 5.radio frequency trasmitter
3.Magnetic coils 6.radio frequency receiver
7. read out systems
12. .
1.Sample holder :- it is a glass tube used to hold the
sample and the solvent it is 8.5 cm long and 0.5cm in
diameter
2.permanent magnet:- it is used to provide the
magnetic field this magnetic field id homogeneous in
nature and is u around 60-100MHZ
3.Magnetic coils:- these are electromagnets when there
is a flow of current these magnetic coils generate or
induce magnetic field
4. Sweep generator:- this part of the instrument is used
for maintaining a equal amount of magnetic field
passing through the sample
13. 5.Radio frequency transmitter-: it is the radio
transmitter coil that produces a short powerfull pulse
of radio wave
6.Radio frequency receiver-: it is a radio coil that
detects the radio frequency emitted by the nuclei as
they relax to a lower energy state
7.Readout system-:it is the computer that records and
analyses the data and records are then shown in the
form of graph
14. Solvents used in NMR
The following solvents are used in which hydrogen is
replaced by dueterium
CCl4- carbon tetra chloride
CS2 –carbon disulphide
CDCl3- Deutieiochloroform
C6D6-Hexa deutriobenzne
D2O- deutrim oxide
18. Working of NMR
The sample is dissolved in a solvent usually CDCl3and
placed in a magnetic field
A radiofrequency generator then irradiates the sample
with a short pulse of radiation ,causing resonance of
the proton
When the nuclie fall back to their lowest energy state
,the detector mausres the energy released and a
spectrum is recorded in the form of graph